Expanding the genetic toolkit in Xenopus : approaches and opportunities for human disease modeling

dc.contributor.author Tandon, Panna
dc.contributor.author Conlon, Frank
dc.contributor.author Furlow, J. David
dc.contributor.author Horb, Marko E.
dc.date.accessioned 2017-07-17T15:04:41Z
dc.date.available 2017-07-17T15:04:41Z
dc.date.issued 2016-04-22
dc.description © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 325-335, doi:10.1016/j.ydbio.2016.04.009. en_US
dc.description.abstract The amphibian model Xenopus, has been used extensively over the past century to study multiple aspects of cell and developmental biology. Xenopus offers advantages of a non-mammalian system, including high fecundity, external development, and simple housing requirements, with additional advantages of large embryos, highly conserved developmental processes, and close evolutionary relationship to higher vertebrates. There are two main species of Xenopus used in biomedical research, Xenopus laevis and Xenopus tropicalis; the common perception is that both species are excellent models for embryological and cell biological studies, but only Xenopus tropicalis is useful as a genetic model. The recent completion of the Xenopus laevis genome sequence combined with implementation of genome editing tools, such as TALENs (transcription activator-like effector nucleases) and CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated nucleases), greatly facilitates the use of both Xenopus laevis and Xenopus tropicalis for understanding gene function in development and disease. In this paper, we review recent advances made in Xenopus laevis and Xenopus tropicalis with TALENs and CRISPR-Cas and discuss the various approaches that have been used to generate knockout and knock-in animals in both species. These advances show that both Xenopus species are useful for genetic approaches and in particular counters the notion that Xenopus laevis is not amenable to genetic manipulations. en_US
dc.description.sponsorship This work was supported by the National Institutes of Health (P40 OD010997 to M.E.H., R01 HD084409 to M.E.H., R01 HL112618 to P.T. and F.C., and R01 HL127640 to P.T. and F.C.; and the U.S. Environmental Protection Agency (G11E10367 to D.F.). en_US
dc.identifier.citation Developmental Biology 426 (2017): 325-335 en_US
dc.identifier.doi 10.1016/j.ydbio.2016.04.009
dc.identifier.uri https://hdl.handle.net/1912/9107
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.relation.uri https://doi.org/10.1016/j.ydbio.2016.04.009
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject CRISPR-Cas en_US
dc.subject TALENs en_US
dc.subject J strain en_US
dc.subject Xenopus laevis en_US
dc.subject Xenopus tropicalis en_US
dc.subject Knock-in en_US
dc.subject Human disease model en_US
dc.title Expanding the genetic toolkit in Xenopus : approaches and opportunities for human disease modeling en_US
dc.type Article en_US
dspace.entity.type Publication
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