Proteins of the glycine decarboxylase complex in the Hydrogenosome of Trichomonas vaginalis Mukherjee, Mandira Brown, Mark T. McArthur, Andrew G. Johnson, Patricia J. 2007-02-01T15:14:30Z 2007-02-01T15:14:30Z 2006-12
dc.description Author Posting. © American Society for Microbiology, 2006. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Eukaryotic Cell 5 (2006): 2062-2071, doi:10.1128/EC.00205-06. en
dc.description.abstract Trichomonas vaginalis is a unicellular eukaryote that lacks mitochondria and contains a specialized organelle, the hydrogenosome, involved in carbohydrate metabolism and iron-sulfur cluster assembly. We report the identification of two glycine cleavage H proteins and a dihydrolipoamide dehydrogenase (L protein) of the glycine decarboxylase complex in T. vaginalis with predicted N-terminal hydrogenosomal presequences. Immunofluorescence analyses reveal that both H and L proteins are localized in hydrogenosomes, providing the first evidence for amino acid metabolism in this organelle. All three proteins were expressed in Escherichia coli and purified to homogeneity. The experimental Km of L protein for the two H proteins were 2.6 µM and 3.7 µM, consistent with both H proteins serving as substrates of L protein. Analyses using purified hydrogenosomes showed that endogenous H proteins exist as monomers and endogenous L protein as a homodimer in their native states. Phylogenetic analyses of L proteins revealed that the T. vaginalis homologue shares a common ancestry with dihydrolipoamide dehydrogenases from the firmicute bacteria, indicating its acquisition via a horizontal gene transfer event independent of the origins of mitochondria and hydrogenosomes. en
dc.description.sponsorship This work was supported by National Institutes of Health (NIH) grants to P.J.J., a Burroughs-Wellcome Molecular Parasitology Award to P.J.J., and an NIH Microbial Pathogenesis Training Grant (2-T32-AI-07323) to M.T.B. A.G.M. was supported by the Marine Biological Laboratory's Program in Global Infectious Disease, funded by the Ellison Medical Foundation. en
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dc.identifier.citation Eukaryotic Cell 5 (2006): 2062-2071 en
dc.identifier.doi 10.1128/EC.00205-06
dc.language.iso en_US en
dc.publisher American Society for Microbiology en
dc.title Proteins of the glycine decarboxylase complex in the Hydrogenosome of Trichomonas vaginalis en
dc.type Article en
dspace.entity.type Publication
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