The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei Burbach, J. Peter H. Hellemons, Anita J. C. G. M. Grant, Philip Pant, Harish C. 2015-08-26T15:57:50Z 2015-08-26T15:57:50Z 2015-06-26
dc.description © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Biology Open 4 (2015): 954-960, doi: 10.1242/​bio.012476. en_US
dc.description.abstract Homeodomain transcription factors regulate development of embryos and cellular physiology in adult systems. Paired-type homeodomain genes constitute a subclass that has been particularly implicated in establishment of neuronal identity in the mammalian nervous system. We isolated fragments of eight homeodomain genes of this subclass expressed in the stellate ganglion of the North Atlantic long finned squid Loligo pealei (lp) [Note: Loligo pealei has been officially renamed Doryteuthis pealei. For reasons of uniformity and clarity Loligo pealei (lp) is used here]. Of the most abundant ones, we cloned a full length cDNA which encoded the squid ortholog of the paired-type homeodomain proteins Phox2a/b. The homology of lpPhox2 to invertebrate and mammalian Phox2 was limited to the homeodomain. In contrast to mouse Phox2b, lpPhox2 was unable to transactivate the dopamine beta-hydroxylase (DBH) promoter in a heterologous mammalian transfection system. In vivo, lpPhox2 was expressed in the developing stellate ganglion of stage 27 squid embryos and continued to be expressed in the adult stellate neurons where expression was confined to the giant fiber lobe containing the neurons that form the giant axons. The expression of lpPhox was similarly timed and distributed as the Fmrf gene. Furthermore, the Fmrf upstream region contained putative Phox2a/b binding sites. These results suggest a role of lpPhox2 in the developmental specification of neuronal identity and regulation of neurons of the squid giant axon. en_US
dc.description.sponsorship This research was supported by the David de Wied Foundation and by two Marine Biological Laboratory (MBL) Fellowships (J.P.H.B.) sponsored by the Baxter Postdoctoral Fellowship Fund, MBL Associates Fund, James A. and Faith Miller Memorial Fund, the H.B. Steinbach Fund, the Hersenstichting Nederland (J.P.H.B.) and by the intramural research program of the U.S. National Institutes of Health (NIH), NINDS. USA. en_US
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dc.identifier.citation Biology Open 4 (2015): 954-960 en_US
dc.identifier.doi 10.1242/bio.012476
dc.language.iso en_US en_US
dc.publisher The Company of Biologists en_US
dc.rights Attribution 3.0 Unported
dc.subject Homeodomain transcription factors en_US
dc.subject Squid en_US
dc.subject Phox2 en_US
dc.subject FMRFamide en_US
dc.subject Stellate ganglion en_US
dc.subject Squid giant axon en_US
dc.subject Brain development en_US
dc.title The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei en_US
dc.type Article en_US
dspace.entity.type Publication
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