Quantitative analysis and modeling of katanin function in flagellar length control

dc.contributor.author Kannegaard, Elisa
dc.contributor.author Rego, E. Hesper
dc.contributor.author Schuck, Sebastian
dc.contributor.author Feldman, Jessica L.
dc.contributor.author Marshall, Wallace F.
dc.date.accessioned 2015-01-08T17:23:30Z
dc.date.available 2015-01-08T17:23:30Z
dc.date.issued 2014-08-20
dc.description © The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Biology of the Cell 25 (2014): 3686-3698, doi:10.1091/mbc.E14-06-1116. en_US
dc.description.abstract Flagellar length control in Chlamydomonas reinhardtii provides a simple model system in which to investigate the general question of how cells regulate organelle size. Previous work demonstrated that Chlamydomonas cytoplasm contains a pool of flagellar precursor proteins sufficient to assemble a half-length flagellum and that assembly of full-length flagella requires synthesis of additional precursors to augment the preexisting pool. The regulatory systems that control the synthesis and regeneration of this pool are not known, although transcriptional regulation clearly plays a role. We used quantitative analysis of length distributions to identify candidate genes controlling pool regeneration and found that a mutation in the p80 regulatory subunit of katanin, encoded by the PF15 gene in Chlamydomonas, alters flagellar length by changing the kinetics of precursor pool utilization. This finding suggests a model in which flagella compete with cytoplasmic microtubules for a fixed pool of tubulin, with katanin-mediated severing allowing easier access to this pool during flagellar assembly. We tested this model using a stochastic simulation that confirms that cytoplasmic microtubules can compete with flagella for a limited tubulin pool, showing that alteration of cytoplasmic microtubule severing could be sufficient to explain the effect of the pf15 mutations on flagellar length. en_US
dc.description.sponsorship This work was funded by National Institutes of Health Grant R01 GM097017. en_US
dc.format.mimetype application/pdf
dc.identifier.citation Molecular Biology of the Cell 25 (2014): 3686-3698 en_US
dc.identifier.doi 10.1091/mbc.E14-06-1116
dc.identifier.uri https://hdl.handle.net/1912/7034
dc.language.iso en_US en_US
dc.publisher American Society for Cell Biology en_US
dc.relation.uri https://doi.org/10.1091/mbc.E14-06-1116
dc.rights Attribution-NonCommercial-ShareAlike 3.0 Unported *
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0
dc.title Quantitative analysis and modeling of katanin function in flagellar length control en_US
dc.type Article en_US
dspace.entity.type Publication
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relation.isAuthorOfPublication.latestForDiscovery 5bd94804-6f32-4509-9606-e8fd8b29b889
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