Dynamic organization of chromatin domains revealed by super-resolution live-dell imaging

dc.contributor.author Nozaki, Tadasu
dc.contributor.author Imai, Ryosuke
dc.contributor.author Tanbo, Mai
dc.contributor.author Nagashima, Ryosuke
dc.contributor.author Tamura, Sachiko
dc.contributor.author Tani, Tomomi
dc.contributor.author Joti, Yasumasa
dc.contributor.author Tomita, Masaru
dc.contributor.author Hibino, Kayo
dc.contributor.author Kanemaki, Masato T.
dc.contributor.author Wendt, Kerstin S.
dc.date.accessioned 2017-07-28T18:38:33Z
dc.date.available 2018-07-13T09:35:14Z
dc.date.issued 2017-06
dc.description Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here by permission of Cell Press for personal use, not for redistribution. The definitive version was published in Molecular Cell 67 (2017): 282-293, doi:10.1016/j.molcel.2017.06.018. en_US
dc.description.abstract The eukaryotic genome is organized within cells as chromatin. For proper information output, higher-order chromatin structures can be regulated dynamically. How such structures form and behave in various cellular processes remains unclear. Here, by combining super-resolution imaging (photoactivated localization microscopy, PALM) and single nucleosome tracking, we developed a nuclear imaging system to visualize the higher-order structures along with their dynamics in live mammalian cells. We demonstrated that nucleosomes form compact domains with a peak diameter of ~160 nm and move coherently in live cells. The heterochromatin-rich regions showed more domains and less movement. With cell differentiation, the domains became more apparent, with reduced dynamics. Furthermore, various perturbation experiments indicated that they are organized by a combination of factors, including cohesin and nucleosome–nucleosome interactions. Notably, we observed the domains during mitosis, suggesting that they act as building blocks of chromosomes and may serve as information units throughout the cell cycle. en_US
dc.description.embargo 2018-07-13 en_US
dc.description.sponsorship This work was supported by MEXT and JSPS grants (23115005 and 16H04746, respectively) and a JST CREST grant (JPMJCR15G2). en_US
dc.identifier.uri https://hdl.handle.net/1912/9135
dc.language.iso en en_US
dc.relation.uri https://doi.org/10.1016/j.molcel.2017.06.018
dc.subject Chromatin en_US
dc.subject Chromosome en_US
dc.subject Chromatin domain en_US
dc.subject PALM en_US
dc.subject Chromatin dynamics en_US
dc.subject Single nucleosome tracking en_US
dc.title Dynamic organization of chromatin domains revealed by super-resolution live-dell imaging en_US
dc.type Preprint en_US
dspace.entity.type Publication
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