Differential responses of ammonia-oxidizing archaea and bacteria to long-term fertilization in a New England salt marsh

dc.contributor.author Peng, Xuefeng
dc.contributor.author Yando, Erik
dc.contributor.author Hildebrand, Erica
dc.contributor.author Dwyer, Courtney
dc.contributor.author Kearney, Anne
dc.contributor.author Waciega, Alex
dc.contributor.author Valiela, Ivan
dc.contributor.author Bernhard, Anne E.
dc.date.accessioned 2013-02-20T20:06:21Z
dc.date.available 2013-02-20T20:06:21Z
dc.date.issued 2013-01-22
dc.description © The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Aquatic Microbiology 3 (2013): 445, doi:10.3389/fmicb.2012.00445. en_US
dc.description.abstract Since the discovery of ammonia-oxidizing archaea (AOA), new questions have arisen about population and community dynamics and potential interactions between AOA and ammonia-oxidizing bacteria (AOB). We investigated the effects of long-term fertilization on AOA and AOB in the Great Sippewissett Marsh, Falmouth, MA, USA to address some of these questions. Sediment samples were collected from low and high marsh habitats in July 2009 from replicate plots that received low (LF), high (HF), and extra high (XF) levels of a mixed NPK fertilizer biweekly during the growing season since 1974. Additional untreated plots were included as controls (C). Terminal restriction fragment length polymorphism analysis of the amoA genes revealed distinct shifts in AOB communities related to fertilization treatment, but the response patterns of AOA were less consistent. Four AOB operational taxonomic units (OTUs) predictably and significantly responded to fertilization, but only one AOA OTU showed a significant pattern. Betaproteobacterial amoA gene sequences within the Nitrosospira-like cluster dominated at C and LF sites, while sequences related to Nitrosomonas spp. dominated at HF and XF sites. We identified some clusters of AOA sequences recovered primarily from high fertilization regimes, but other clusters consisted of sequences recovered from all fertilization treatments, suggesting greater physiological diversity. Surprisingly, fertilization appeared to have little impact on abundance of AOA or AOB. In summary, our data reveal striking patterns for AOA and AOB in response to long-term fertilization, and also suggest a missing link between community composition and abundance and nitrogen processing in the marsh. en_US
dc.description.sponsorship This work was supported in part by the National Science Foundation award DEB-0814586 (to Anne E. Bernhard). Additional support was provided by the George and Carol Milne Endowment at Connecticut College. en_US
dc.format.mimetype application/pdf
dc.identifier.citation Frontiers in Aquatic Microbiology 3 (2013): 445 en_US
dc.identifier.doi 10.3389/fmicb.2012.00445
dc.identifier.uri https://hdl.handle.net/1912/5781
dc.language.iso en_US en_US
dc.publisher Frontiers Media en_US
dc.relation.uri https://doi.org/10.3389/fmicb.2012.00445
dc.rights Attribution 3.0 Unported *
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject AmoA en_US
dc.subject TRFLP en_US
dc.subject Great Sippewissett Marsh en_US
dc.subject Fertilization en_US
dc.subject Salt marsh en_US
dc.title Differential responses of ammonia-oxidizing archaea and bacteria to long-term fertilization in a New England salt marsh en_US
dc.type Article en_US
dspace.entity.type Publication
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