Expanding dispersal studies at hydrothermal vents through species identification of cryptic larval forms

dc.contributor.author Adams, Diane K.
dc.contributor.author Mills, Susan W.
dc.contributor.author Shank, Timothy M.
dc.contributor.author Mullineaux, Lauren S.
dc.date.accessioned 2010-10-07T15:52:41Z
dc.date.available 2011-01-18T09:22:55Z
dc.date.issued 2010-01
dc.description Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Marine Biology 157 (2010): 1049-1062, doi:10.1007/s00227-009-1386-8. en_US
dc.description.abstract The rapid identification of hydrothermal vent-endemic larvae to the species level is a key limitation to understanding the dynamic processes that control the abundance and distribution of fauna in such a patchy and ephemeral environment. Many larval forms collected near vents, even those in groups such as gastropods that often form a morphologically distinct larval shell, have not been identified to species. We present a staged approach that combines morphological and molecular identification to optimize the capability, efficiency, and economy of identifying vent gastropod larvae from the northern East Pacific Rise (NEPR). With this approach, 15 new larval forms can be identified to species. A total of 33 of the 41 gastropod species inhabiting the NEPR, and 26 of the 27 gastropod species known to occur specifically in the 9° 50’ N region, can be identified to species. Morphological identification efforts are improved by new protoconch descriptions for Gorgoleptis spiralis, Lepetodrilus pustulosus, Nodopelta subnoda, and Echinopelta fistulosa. Even with these new morphological descriptions, the majority of lepetodrilids and peltospirids require molecular identification. Restriction fragment length polymorphism digests are presented as an economical method for identification of five species of Lepetodrilus and six species of peltospirids. The remaining unidentifiable specimens can be assigned to species by comparison to an expanded database of 18S ribosomal DNA. The broad utility of the staged approach was exemplified by the revelation of species-level variation in daily planktonic samples and the identification and characterization of egg capsules belonging to a conid gastropod Gymnobela sp. A. The improved molecular and morphological capabilities nearly double the number of species amenable to field studies of dispersal and population connectivity. en_US
dc.description.sponsorship Funding was provided by as Woods Hole Oceanographic Institution Deep Ocean Exploration Institute grant to L.M and S. Beaulieu, National Science Foundation grants OCE-0424953, OCE-9712233, and OCE-9619605 to L.M, OCE-0327261 to T.S., and OCE-0002458 to K. Von Damm, and a National Defense Science and Engineering Graduate fellowship to D.A. en_US
dc.format.mimetype application/pdf
dc.identifier.uri https://hdl.handle.net/1912/3935
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1007/s00227-009-1386-8
dc.subject Hydrothermal vent en_US
dc.subject Larvae en_US
dc.subject Protoconch en_US
dc.subject Gastropod en_US
dc.subject Lepetodrilus en_US
dc.subject Peltospira en_US
dc.subject RFLP en_US
dc.subject Barcode en_US
dc.subject Egg capsules en_US
dc.title Expanding dispersal studies at hydrothermal vents through species identification of cryptic larval forms en_US
dc.type Preprint en_US
dspace.entity.type Publication
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