Biological data of mussel larvae treated with fluorescent dyes and grown in two pH treatments.

dc.contributor.author Kapsenberg, Lydia
dc.coverage.spatial NW Mediterranean Sea
dc.date.accessioned 2021-01-22T19:50:09Z
dc.date.available 2021-01-22T19:50:09Z
dc.date.created 2018-12-20
dc.date.issued 2021-01-22
dc.description Dataset: larval responses to pH variability - staining
dc.description.abstract Mussel larvae of Mytilus galloprovincialis were grown in two pH treatments (pH 8.1 and 7.4). Larvae were collected for biological measurements of shell field development and calcification at 35 hours post-fertilization (hpf, trochophore stage). Calcein dye was added to the cultures prior to the start of calcification. Calcofluor is live dye and so was added to sampled larvae at 35 hpf for immediate imaging. Confocal microscopy was used for 3D imaging of larvae. Images were processed in ImageJ. Shell field area was determined as the area stained by calcofluor, on one valve. Calcification area was determined as the area stained by calcein, on one valve. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/751258
dc.description.sponsorship NSF Division of Ocean Sciences (NSF OCE) OCE-1521597
dc.identifier.citation Kapsenberg, L. (2021). Biological data of mussel larvae treated with fluorescent dyes and grown in two pH treatments. (Version 1) [Data set]. Biological and Chemical Oceanography Data Management Office (BCO-DMO). https://doi.org/10.26008/1912/BCO-DMO.751258.1
dc.identifier.doi 10.26008/1912/bco-dmo.751258.1
dc.identifier.uri https://hdl.handle.net/1912/26610
dc.language.iso en_US
dc.publisher Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
dc.relation.uri http://lod.bco-dmo.org/id/dataset/751258
dc.relation.uri https://doi.org/10.26008/1912/bco-dmo.751258.1
dc.rights Creative Commons Attribution 4.0
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Mytilus galloprovincialis
dc.subject Mussels
dc.subject PH
dc.subject Larvae
dc.title Biological data of mussel larvae treated with fluorescent dyes and grown in two pH treatments.
dc.type Dataset
dspace.entity.type Publication
relation.isAuthorOfPublication c11e2276-abe9-4ccf-88b1-6cacb0a346d2
relation.isAuthorOfPublication.latestForDiscovery c11e2276-abe9-4ccf-88b1-6cacb0a346d2
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