Microbial diversity of hydrothermal sediments in the Guaymas Basin : evidence for anaerobic methanotrophic communities

dc.contributor.author Teske, Andreas
dc.contributor.author Hinrichs, Kai-Uwe
dc.contributor.author Edgcomb, Virginia P.
dc.contributor.author de Vera Gomez, Alvin
dc.contributor.author Kysela, David
dc.contributor.author Sylva, Sean P.
dc.contributor.author Sogin, Mitchell L.
dc.contributor.author Jannasch, Holger W.
dc.date.accessioned 2005-11-23T18:30:34Z
dc.date.available 2005-11-23T18:30:34Z
dc.date.issued 2002-04
dc.description Author Posting. © American Society for Microbiology, 2002. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 68 (2002): 1994-2007, doi:10.1128/AEM.68.4.1994-2007.2002.
dc.description.abstract Microbial communities in hydrothermally active sediments of the Guaymas Basin (Gulf of California, Mexico) were studied by using 16S rRNA sequencing and carbon isotopic analysis of archaeal and bacterial lipids. The Guaymas sediments harbored uncultured euryarchaeota of two distinct phylogenetic lineages within the anaerobic methane oxidation 1 (ANME-1) group, ANME-1a and ANME-1b, and of the ANME-2c lineage within the Methanosarcinales, both previously assigned to the methanotrophic archaea. The archaeal lipids in the Guaymas Basin sediments included archaeol, diagnostic for nonthermophilic euryarchaeota, and sn-2-hydroxyarchaeol, with the latter compound being particularly abundant in cultured members of the Methanosarcinales. The concentrations of these compounds were among the highest observed so far in studies of methane seep environments. The δ-13C values of these lipids (δ-13C = -89 to -58%) indicate an origin from anaerobic methanotrophic archaea. This molecular-isotopic signature was found not only in samples that yielded predominantly ANME-2 clones but also in samples that yielded exclusively ANME-1 clones. ANME-1 archaea therefore remain strong candidates for mediation of the anaerobic oxidation of methane. Based on 16S rRNA data, the Guaymas sediments harbor phylogenetically diverse bacterial populations, which show considerable overlap with bacterial populations of geothermal habitats and natural or anthropogenic hydrocarbon-rich sites. Consistent with earlier observations, our combined evidence from bacterial phylogeny and molecular-isotopic data indicates an important role of some novel deeply branching bacteria in anaerobic methanotrophy. Anaerobic methane oxidation likely represents a significant and widely occurring process in the trophic ecology of methane-rich hydrothermal vents. This study stresses a high diversity among communities capable of anaerobic oxidation of methane. en
dc.description.sponsorship Andreas Teske was supported by DFG postdoctoral fellowship 262-1/1 and a subsequent WHOI postdoctoral fellowship. en
dc.format.extent 2494648 bytes
dc.format.mimetype application/pdf
dc.identifier.citation Applied and Environmental Microbiology 68 (2002): 1994-2007 en
dc.identifier.doi 10.1128/AEM.68.4.1994-2007.2002
dc.identifier.uri https://hdl.handle.net/1912/186
dc.language.iso en_US en
dc.publisher American Society for Microbiology en
dc.relation.uri https://doi.org/10.1128/AEM.68.4.1994-2007.2002
dc.subject Anaerobic methanotrophy en
dc.subject Anaerobic methane oxidation en
dc.title Microbial diversity of hydrothermal sediments in the Guaymas Basin : evidence for anaerobic methanotrophic communities en
dc.type Article en
dspace.entity.type Publication
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