Mitochondrial respiration and Ca2+ waves are linked during fertilization and meiosis completion Dumollard, Remi Hammar, Katherine M. Porterfield, D. Marshall Smith, Peter J. S. Cibert, Christian Rouviere, Christian Sardet, Christian 2005-11-25T20:37:17Z 2005-11-25T20:37:17Z 2003
dc.description Author Posting. © Company of Biologists Limited, 2003. This article is posted here by permission of Company of Biologists Limited for personal use, not for redistribution. The definitive version was published in Development 130 (2003): 683-692, doi:10.1242/10.1242/dev.00296.
dc.description.abstract Fertilization increases both cytosolic Ca2+ concentration and oxygen consumption in the egg but the relationship between these two phenomena remains largely obscure. We have measured mitochondrial oxygen consumption and the mitochondrial NADH concentration on single ascidian eggs and found that they increase in phase with each series of meiotic Ca2+ waves emitted by two pacemakers (PM1 and PM2). Oxygen consumption also increases in response to Ins(1,4,5)P3-induced Ca2+ transients. Using mitochondrial inhibitors we show that active mitochondria sequester cytosolic Ca2+ during sperm-triggered Ca2+ waves and that they are strictly necessary for triggering and sustaining the activity of the meiotic Ca2+ wave pacemaker PM2. Strikingly, the activity of the Ca2+ wave pacemaker PM2 can be restored or stimulated by flash photolysis of caged ATP. Taken together our observations provide the first evidence that, in addition to buffering cytosolic Ca2+, the egg's mitochondria are stimulated by Ins(1,4,5)P3-mediated Ca2+ signals. In turn, mitochondrial ATP production is required to sustain the activity of the meiotic Ca2+ wave pacemaker PM2. en
dc.description.sponsorship This work was supported by AFM and ARC grants to C. S. en
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dc.identifier.citation Development 130 (2003): 683-692 en
dc.identifier.doi 10.1242/dev.00296
dc.language.iso en en
dc.publisher Company of Biologists Limited en
dc.subject Fertilization en
dc.subject Respiration en
dc.subject Ca2+ waves en
dc.subject Mitochondria en
dc.subject Endoplasmic reticulum en
dc.subject ATP en
dc.subject Ca2+ wave pacemakers en
dc.subject Ascidian en
dc.title Mitochondrial respiration and Ca2+ waves are linked during fertilization and meiosis completion en
dc.type Article en
dspace.entity.type Publication
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