Multiplexed Microfluidic Screening of Bacterial Chemotaxis Modeling Results from 2019-2022 (VIC project)

dc.contributor.author Henshaw, Richard J.
dc.coverage.spatial Culture-based work
dc.coverage.temporal 20190901 - 20220715 (UTC)
dc.date.accessioned 2023-10-04T19:35:19Z
dc.date.available 2023-10-04T19:35:19Z
dc.date.created 2022-12-21
dc.date.issued 2023-10-03
dc.description Dataset: Multiplexed microfluidic screening of bacterial chemotaxis
dc.description.abstract Microorganism sensing of and responding to ambient chemical gradients regulates a myriad of microbial processes that are fundamental to ecosystem function and human health and disease. The development of efficient, high-throughput screening tools for microbial chemotaxis is essential to disentangling the roles of diverse chemical compounds and concentrations that control cell nutrient uptake, chemorepulsion from toxins, and microbial pathogenesis. Here, we present a novel microfluidic multiplexed chemotaxis device (MCD), which uses serial dilution to simultaneously perform six parallel bacterial chemotaxis assays that span five orders of magnitude in chemostimulant concentration on a single chip. We first validated the dilution and gradient generation performance of the MCD, and then compared the measured chemotactic response of an established bacterial chemotaxis system (Vibrio alginolyticus) to a standard microfluidic assay. Next, the MCD's versatility was assessed by quantifying the chemotactic responses of different bacteria (Psuedoalteromonas haloplanktis, Escherichia coli) to different chemoattractants and chemorepellents. The MCD vastly accelerates the chemotactic screening process, which is critical to deciphering the complex sea of chemical stimuli underlying microbial responses. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/885701
dc.description.sponsorship NSF Division of Ocean Sciences (NSF OCE) OCE-1829905, NSF Division of Ocean Sciences (NSF OCE) OCE-1829827
dc.identifier.citation Henshaw, R. J. (2023). Multiplexed Microfluidic Screening of Bacterial Chemotaxis Modeling Results from 2019-2022 (VIC project) (Version 1) [Data set]. Biological and Chemical Oceanography Data Management Office (BCO-DMO). https://doi.org/10.26008/1912/BCO-DMO.885701.1
dc.identifier.doi 10.26008/1912/bco-dmo.885701.1
dc.identifier.uri https://hdl.handle.net/1912/66960
dc.language.iso en_US
dc.publisher Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
dc.relation.uri http://lod.bco-dmo.org/id/dataset/885701
dc.relation.uri https://doi.org/10.26008/1912/bco-dmo.885701.1
dc.rights Creative Commons Attribution 4.0
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject microfluidics
dc.subject micromixing
dc.subject chemotaxis
dc.title Multiplexed Microfluidic Screening of Bacterial Chemotaxis Modeling Results from 2019-2022 (VIC project)
dc.type Dataset
dspace.entity.type Publication
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mcd_chemicalconcentration_gradient_measurements-2.csv
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