Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

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Peng, Hanchuan
Tang, Jianyong
Xiao, Hang
Bria, Alessandro
Zhou, Jianlong
Butler, Victoria
Zhou, Zhi
Gonzalez-Bellido, Paloma T.
Oh, Seung W.
Chen, Jichao
Mitra, Ananya
Tsien, Richard W.
Zeng, Hongkui
Ascoli, Giorgio A.
Iannello, Giulio
Hawrylycz, Michael
Myers, Eugene
Long, Fuhui
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Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain.
© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Nature Communications 5 (2014): 4342, doi:10.1038/ncomms5342.
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Nature Communications 5 (2014): 4342
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