Transcriptome of the Caribbean stony coral Porites astreoides from three developmental stages Mansour, Tamer A. Rosenthal, Joshua J. C. Brown, C. Titus Roberson, Loretta M. 2016-09-22T15:19:12Z 2016-09-22T15:19:12Z 2016-08-02
dc.description © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in GigaScience 5 (2016): 33, doi:10.1186/s13742-016-0138-1. en_US
dc.description.abstract Porites astreoides is a ubiquitous species of coral on modern Caribbean reefs that is resistant to increasing temperatures, overfishing, and other anthropogenic impacts that have threatened most other coral species. We assembled and annotated a transcriptome from this coral using Illumina sequences from three different developmental stages collected over several years: free-swimming larvae, newly settled larvae, and adults (>10 cm in diameter). This resource will aid understanding of coral calcification, larval settlement, and host–symbiont interactions. A de novo transcriptome for the P. astreoides holobiont (coral plus algal symbiont) was assembled using 594 Mbp of raw Illumina sequencing data generated from five age-specific cDNA libraries. The new transcriptome consists of 867 255 transcript elements with an average length of 685 bases. The isolated P. astreoides assembly consists of 129 718 transcript elements with an average length of 811 bases, and the isolated Symbiodinium sp. assembly had 186 177 transcript elements with an average length of 1105 bases. This contribution to coral transcriptome data provides a valuable resource for researchers studying the ontogeny of gene expression patterns within both the coral and its dinoflagellate symbiont. en_US
dc.description.sponsorship Bioinformatic analysis was performed in part on computing resources at the University of Puerto Rico (UPR) Puerto Rico Center for Environmental Neuroscience (PRCEN)’s High Performance Computing Facility, which is supported by: Institutional Development Award Networks of Biomedical Research Excellent (INBRE) grant P20GM103475 from the National Institute of General Medical Sciences, National Institutes of Health; the Institute for Functional Nanomaterials (IFN) award from the Experimental Program to Stimulate Competitive Research (EPSCoR) Track 1 program of the National Science Foundation (NSF); and EPSCoR Track 2 awards for computational nanoscience (EPS 1002410, EPS 1010094). Funding and support of the research was provided by PRCEN thanks to an NSF Centers of Research Excellent in Science and Technology (CREST) award, number HRD-1137725. en_US
dc.identifier.citation GigaScience 5 (2016): 33 en_US
dc.identifier.doi 10.1186/s13742-016-0138-1
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.rights Attribution 4.0 International *
dc.rights.uri *
dc.subject Porites astreoides en_US
dc.subject Calcification en_US
dc.subject Biomineralization en_US
dc.subject Coral en_US
dc.subject Symbiodinium en_US
dc.subject Dinoflagellate en_US
dc.subject Zooxanthellae en_US
dc.subject Symbiosis en_US
dc.subject Swimming larvae en_US
dc.subject Larval settlement en_US
dc.title Transcriptome of the Caribbean stony coral Porites astreoides from three developmental stages en_US
dc.type Article en_US
dspace.entity.type Publication
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