Affinity purification of label-free tubulins from xenopus egg extracts

Thumbnail Image
Date
2020-12-18
Authors
Reusch, Sebastian
 Biswas, Abin
 Hirst, William G.
 Reber, Simone
Linked Authors
Person
Person
Person
Person
Files
1-s2.0-S2666166720301386-main.pdf (3.7 MB)
Alternative Title
Citable URI
https://hdl.handle.net/1912/26818
As Published
https://doi.org/10.1016/j.xpro.2020.100151
Date Created
Location
DOI
10.1016/j.xpro.2020.100151
Related Materials
Replaces
Replaced By
Keywords
Abstract
Cytoplasmic extracts from unfertilized Xenopus eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these in vitro studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologically relevant Xenopus tubulins in milligram yield, which are a complex mixture of isoforms with various post-translational modifications. The protocol is applicable to any cell or tissue of interest. For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020).
Description
© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Reusch, S., Biswas, A., Hirst, W. G., & Reber, S. Affinity purification of label-free tubulins from xenopus egg extracts. STAR Protocols, 1(3), (2020): 100151, doi:10.1016/j.xpro.2020.100151.
Embargo Date
Citation
Reusch, S., Biswas, A., Hirst, W. G., & Reber, S. (2020). Affinity purification of label-free tubulins from xenopus egg extracts. STAR Protocols, 1(3), 100151.
Cruises
Cruise ID
Cruise DOI
Vessel Name
Collections
Visiting Investigators
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International