Development and application of a monoclonal-antibody technique for counting Aureococcus anophagefferens, an alga causing recurrent brown tides in the Mid-Atlantic United States Caron, David A. Dennett, Mark R. Moran, Dawn M. Schaffner, Rebecca A. Lonsdale, Darcy J. Gobler, Christopher J. Nuzzi, Robert McLean, Tim I. 2005-11-23T19:02:30Z 2005-11-23T19:02:30Z 2003-09
dc.description Author Posting. © American Society for Microbiology, 2003. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 69 (2003): 5492-5502, doi:10.1128/AEM.69.9.5492-5502.2003.
dc.description.abstract A method was developed for the rapid detection and enumeration of Aureococcus anophagefferens, the cause of harmful algal blooms called "brown tides" in estuaries of the Mid-Atlantic United States. The method employs a monoclonal antibody (MAb) and a colorimetric, enzyme-linked immunosorbent assay format. The MAb obtained exhibits high reactivity with A. anophagefferens and very low cross-reactivities with a phylogenetically diverse array of other protists and bacteria. Standard curves are constructed for each 96-well microtiter plate by using known amounts of a preserved culture of A. anophagefferens. This approach allows estimation of the abundance of the alga in natural samples. The MAb method was compared to an existing method that employs polyclonal antibodies and epifluorescence microscopy and to direct microscopic counts of A. anophagefferens in samples with high abundances of the alga. The MAb method provided increased quantitative accuracy and greatly reduced sample processing time. A spatial survey of several Long Island estuaries in May 2000 using this new approach documented a range of abundances of A. anophagefferens in these bays spanning nearly 3 orders of magnitude. en
dc.description.sponsorship This work was supported by a grant from The Seaver Institute. en
dc.format.extent 3387230 bytes
dc.format.mimetype application/pdf
dc.identifier.citation Applied and Environmental Microbiology 69 (2003): 5492-5502 en
dc.identifier.doi 10.1128/AEM.69.9.5492-5502.2003
dc.language.iso en_US en
dc.publisher American Society for Microbiology en
dc.subject Aureococcus anophagefferens en
dc.subject Harmful algal blooms (HAB) en
dc.subject Monoclonal antibody (MAb) en
dc.title Development and application of a monoclonal-antibody technique for counting Aureococcus anophagefferens, an alga causing recurrent brown tides in the Mid-Atlantic United States en
dc.type Article en
dspace.entity.type Publication
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