Genetic tool development in marine protists: emerging model organisms for experimental cell biology

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2020-04-06
Authors
Faktorová, Drahomíra
Nisbet, R. Ellen R.
Fernández Robledo, José A.
Casacuberta, Elena
Sudek, Lisa
Allen, Andrew E.
Ares, Manuel, Jr.
Aresté, Cristina
Balestreri, Cecilia
Barbrook, Adrian C.
Beardslee, Patrick
Bender, Sara J.
Booth, David S.
Bouget, François-Yves
Bowler, Chris
Breglia, Susana A.
Brownlee, Colin
Burger, Gertraud
Cerutti, Heriberto
Cesaroni, Rachele
Chiurillo, Miguel A.
Clemente, Thomas
Coles, Duncan B.
Collier, Jackie L.
Cooney, Elizabeth C.
Coyne, Kathryn J.
Docampo, Roberto
Dupont, Christopher L.
Edgcomb, Virginia P.
Einarsso, Elin
Elustondo, Pía A.
Federici, Fernan
Freire-Beneitez, Veronica
Freyria, Nastasia J.
Fukuda, Kodai
García, Paulo A.
Girguis, Peter R.
Gomaa, Fatma
Gornik, Sebastian G.
Guo, Jian
Hampl, Vladimír
Hanawa, Yutaka
Haro-Contreras, Esteban R.
Hehenberger, Elisabeth
Highfield, Andrea
Hirakawa, Yoshihisa
Hopes, Amanda
Howe, Christopher J.
Hu, Ian
Ibañez, Jorge
Irwin, Nicholas A. T.
Ishii, Yuu
Janowicz, Natalia Ewa
Jones, Adam C.
Kachale, Ambar
Fujimura-Kamada, Konomi
Kaur, Binnypreet
Kaye, Jonathan Z.
Kazana, Eleanna
Keeling, Patrick J.
King, Nicole
Klobutcher, Lawrence A.
Lander, Noelia
Lassadi, Imen
Li, Zhuhong
Lin, Senjie
Lozano, Jean-Claude
Luan, Fulei
Maruyama, Shinichiro
Matute, Tamara
Miceli, Cristina
Minagawa, Jun
Moosburner, Mark
Najle, Sebastián R.
Nanjappa, Deepak
Nimmo, Isabel C.
Noble, Luke
Novák Vanclová, Anna M. G.
Nowacki, Mariusz
Nuñez, Isaac
Pain, Arnab
Piersanti, Angela
Pucciarelli, Sandra
Pyrih, Jan
Rest, Joshua S.
Rius, Mariana
Robertson, Deborah
Ruaud, Albane
Ruiz-Trillo, Iñaki
Sigg, Monika A.
Silver, Pamela A.
Slamovits, Claudio H.
Smith, G. Jason
Sprecher, Brittany N.
Stern, Rowena
Swart, Estienne C.
Tsaousis, Anastasios D.
Tsypin, Lev
Turkewitz, Aaron
Turnšek, Jernej
Valach, Matus
Vergé, Valérie
von Dassow, Peter
von der Haar, Tobias
Waller, Ross F.
Wang, Lu
Wen, Xiaoxue
Wheeler, Glen L.
Woods, April
Zhang, Huan
Mock, Thomas
Worden, Alexandra Z.
Lukes, Julius
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DOI
10.1038/s41592-020-0796-x
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Abstract
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
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© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Faktorova, D., Nisbet, R. E. R., Robledo, J. A. F., Casacuberta, E., Sudek, L., Allen, A. E., Ares, M., Jr., Areste, C., Balestreri, C., Barbrook, A. C., Beardslee, P., Bender, S., Booth, D. S., Bouget, F., Bowler, C., Breglia, S. A., Brownlee, C., Burger, G., Cerutti, H., Cesaroni, R., Chiurillo, M. A., Clemente, T., Coles, D. B., Collier, J. L., Cooney, E. C., Coyne, K., Docampo, R., Dupont, C. L., Edgcomb, V., Einarsson, E., Elustondo, P. A., Federici, F., Freire-Beneitez, V., Freyria, N. J., Fukuda, K., Garcia, P. A., Girguis, P. R., Gomaa, F., Gornik, S. G., Guo, J., Hampl, V., Hanawa, Y., Haro-Contreras, E. R., Hehenberger, E., Highfield, A., Hirakawa, Y., Hopes, A., Howe, C. J., Hu, I., Ibanez, J., Irwin, N. A. T., Ishii, Y., Janowicz, N. E., Jones, A. C., Kachale, A., Fujimura-Kamada, K., Kaur, B., Kaye, J. Z., Kazana, E., Keeling, P. J., King, N., Klobutcher, L. A., Lander, N., Lassadi, I., Li, Z., Lin, S., Lozano, J., Luan, F., Maruyama, S., Matute, T., Miceli, C., Minagawa, J., Moosburner, M., Najle, S. R., Nanjappa, D., Nimmo, I. C., Noble, L., Vanclova, A. M. G. N., Nowacki, M., Nunez, I., Pain, A., Piersanti, A., Pucciarelli, S., Pyrih, J., Rest, J. S., Rius, M., Robertson, D., Ruaud, A., Ruiz-Trillo, I., Sigg, M. A., Silver, P. A., Slamovits, C. H., Smith, G. J., Sprecher, B. N., Stern, R., Swart, E. C., Tsaousis, A. D., Tsypin, L., Turkewitz, A., Turnsek, J., Valach, M., Verge, V., von Dassow, P., von der Haar, T., Waller, R. F., Wang, L., Wen, X., Wheeler, G., Woods, A., Zhang, H., Mock, T., Worden, A. Z., & Lukes, J. Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods, 17, (2020): 481-494, doi:10.1038/s41592-020-0796-x.
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Faktorova, D., Nisbet, R. E. R., Robledo, J. A. F., Casacuberta, E., Sudek, L., Allen, A. E., Ares, M., Jr., Areste, C., Balestreri, C., Barbrook, A. C., Beardslee, P., Bender, S., Booth, D. S., Bouget, F., Bowler, C., Breglia, S. A., Brownlee, C., Burger, G., Cerutti, H., Cesaroni, R., Chiurillo, M. A., Clemente, T., Coles, D. B., Collier, J. L., Cooney, E. C., Coyne, K., Docampo, R., Dupont, C. L., Edgcomb, V., Einarsson, E., Elustondo, P. A., Federici, F., Freire-Beneitez, V., Freyria, N. J., Fukuda, K., Garcia, P. A., Girguis, P. R., Gomaa, F., Gornik, S. G., Guo, J., Hampl, V., Hanawa, Y., Haro-Contreras, E. R., Hehenberger, E., Highfield, A., Hirakawa, Y., Hopes, A., Howe, C. J., Hu, I., Ibanez, J., Irwin, N. A. T., Ishii, Y., Janowicz, N. E., Jones, A. C., Kachale, A., Fujimura-Kamada, K., Kaur, B., Kaye, J. Z., Kazana, E., Keeling, P. J., King, N., Klobutcher, L. A., Lander, N., Lassadi, I., Li, Z., Lin, S., Lozano, J., Luan, F., Maruyama, S., Matute, T., Miceli, C., Minagawa, J., Moosburner, M., Najle, S. R., Nanjappa, D., Nimmo, I. C., Noble, L., Vanclova, A. M. G. N., Nowacki, M., Nunez, I., Pain, A., Piersanti, A., Pucciarelli, S., Pyrih, J., Rest, J. S., Rius, M., Robertson, D., Ruaud, A., Ruiz-Trillo, I., Sigg, M. A., Silver, P. A., Slamovits, C. H., Smith, G. J., Sprecher, B. N., Stern, R., Swart, E. C., Tsaousis, A. D., Tsypin, L., Turkewitz, A., Turnsek, J., Valach, M., Verge, V., von Dassow, P., von der Haar, T., Waller, R. F., Wang, L., Wen, X., Wheeler, G., Woods, A., Zhang, H., Mock, T., Worden, A. Z., & Lukes, J. (2020). Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods, 17, 481-494.
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