Giant reverse transcriptase-encoding transposable elements at telomeres

dc.contributor.author Arkhipova, Irina R.
dc.contributor.author Yushenova, Irina A.
dc.contributor.author Rodriguez, Fernando
dc.date.accessioned 2017-09-27T19:24:04Z
dc.date.available 2018-05-30T08:55:17Z
dc.date.issued 2017-05
dc.description Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here by permission of Oxford University Press for personal use, not for redistribution. The definitive version was published in Molecular Biology and Evolution 34 (2017): 2245–2257, doi:10.1093/molbev/msx159. en_US
dc.description.abstract Transposable elements are omnipresent in eukaryotic genomes and have a profound impact on chromosome structure, function and evolution. Their structural and functional diversity is thought to be reasonably well-understood, especially in retroelements, which transpose via an RNA intermediate copied into cDNA by the element-encoded reverse transcriptase, and are characterized by a compact structure. Here we report a novel type of expandable eukaryotic retroelements, which we call Terminons. These elements can attach to G-rich telomeric repeat overhangs at the chromosome ends, in a process apparently facilitated by complementary C-rich repeats at the 3’-end of the RNA template immediately adjacent to a hammerhead ribozyme motif. Terminon units, which can exceed 40 kb in length, display an unusually complex and diverse structure, and can form very long chains, with host genes often captured between units. As the principal polymerizing component, Terminons contain Athena reverse transcriptases previously described in bdelloid rotifers and belonging to the enigmatic group of Penelope-like elements, but can additionally accumulate multiple co-oriented ORFs, including DEDDy 3’-exonucleases, GDSL esterases/lipases, GIY-YIG-like endonucleases, rolling-circle replication initiator (Rep) proteins, and putatively structural ORFs with coiled-coil motifs and transmembrane domains. The extraordinary length and complexity of Terminons and the high degree of inter-family variability in their ORF content challenge the current views on the structural organization of eukaryotic retroelements, and highlight their possible connections with the viral world and the implications for the elevated frequency of gene transfer. en_US
dc.description.embargo 2018-05-30 en_US
dc.description.sponsorship This work was supported by the National Institutes of Health (grant GM111917 to I.A.). en_US
dc.identifier.uri https://hdl.handle.net/1912/9252
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1093/molbev/msx159
dc.subject Retrotransposons en_US
dc.subject Bdelloid rotifers en_US
dc.subject Hammerhead ribozymes en_US
dc.subject Horizontal gene transfer en_US
dc.title Giant reverse transcriptase-encoding transposable elements at telomeres en_US
dc.type Preprint en_US
dspace.entity.type Publication
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relation.isAuthorOfPublication.latestForDiscovery 0cdb2934-560e-433c-bc54-9b4c4f502bce
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