Visualizing calcium flux in freely moving nematode embryos

Thumbnail Image
Date
2017-02
Authors
Ardiel, Evan L.
Kumar, Abhishek
Marbach, Joseph
Christensen, Ryan
Gupta, Rishi
Duncan, William
Daniels, Jonathan S.
Stuurman, Nico
Colón-Ramos, Daniel
Shroff, Hari
Linked Authors
Alternative Title
Date Created
Location
DOI
Replaced By
Keywords
Abstract
The lack of physiological recordings from Caenorhabditis elegans embryos stands in stark contrast to the comprehensive anatomical and gene expression datasets already available. Using light-sheet fluorescence microscopy (LSFM) to address the challenges associated with functional imaging at this developmental stage, we recorded calcium dynamics in muscles and neurons and developed analysis strategies to relate activity and movement. In muscles, we found that the initiation of twitching was associated with a spreading calcium wave in a dorsal muscle bundle. Correlated activity in muscle bundles was linked with early twitching and eventual coordinated movement. To identify neuronal correlates of behavior, we monitored brain-wide activity with subcellular resolution and identified a particularly active cell associated with muscle contractions. Finally, imaging neurons of a well-defined adult motor circuit, we found that reversals in the eggshell correlated with calcium transients in AVA interneurons.
Description
Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here by permission of Cell Press for personal use, not for redistribution. The definitive version was published in Biophysical Journal 112 (2017): 1975-1983, doi:10.1016/j.bpj.2017.02.035.
Embargo Date
Citation
Cruises
Cruise ID
Cruise DOI
Vessel Name