Protocol for fluorescent live-cell staining of tardigrades
Protocol for fluorescent live-cell staining of tardigrades
Date
2024-08-09
Authors
Harry, Clayton J.
Hibshman, Jonathan D.
Damatac II, Amor
Davidson, Phillip L.
Estermann, Martin Andres
Flores-Flores, Marycruz
Holmes, Caroline M.
Lazaro, Jorge
Legere, Elizabeth Ann
Leyhr, Jake
Thendral, Siddharthan Balachandar
Vincent, Bridget A.
Goldstein, Bob
Hibshman, Jonathan D.
Damatac II, Amor
Davidson, Phillip L.
Estermann, Martin Andres
Flores-Flores, Marycruz
Holmes, Caroline M.
Lazaro, Jorge
Legere, Elizabeth Ann
Leyhr, Jake
Thendral, Siddharthan Balachandar
Vincent, Bridget A.
Goldstein, Bob
Linked Authors
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DOI
10.1016/j.xpro.2024.103232
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Keywords
Cell Biology
Microscopy
Model Organisms
Microscopy
Model Organisms
Abstract
Tardigrades are microscopic organisms with exceptional resilience to environmental extremes. Most protocols to visualize the internal anatomy of tardigrades rely on fixation, hampering our understanding of dynamic changes to organelles and other subcellular components. Here, we provide protocols for staining live tardigrade adults and other postembryonic stages, facilitating real-time visualization of structures including lipid droplets, mitochondria, lysosomes, and DNA.
Description
© The Author(s), 2024. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Harry, C. J., Hibshman, J. D., Damatac, A. 2nd, Davidson, P. L., Estermann, M. A., Flores-Flores, M., Holmes, C. M., Lázaro, J., Legere, E.-A., Leyhr, J., Thendral, S. B., Vincent, B. A., & Goldstein, B. (2024). Protocol for fluorescent live-cell staining of tardigrades. STAR Protocols, 5(3), 103232, https://doi.org/10.1016/j.xpro.2024.103232.
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Citation
Harry, C. J., Hibshman, J. D., Damatac, A. 2nd, Davidson, P. L., Estermann, M. A., Flores-Flores, M., Holmes, C. M., Lázaro, J., Legere, E.-A., Leyhr, J., Thendral, S. B., Vincent, B. A., & Goldstein, B. (2024). Protocol for fluorescent live-cell staining of tardigrades. STAR Protocols, 5(3), 103232.