Segment number threshold determines juvenile onset of germline cluster expansion in Platynereis dumerilii

dc.contributor.author Kuehn, Emily
dc.contributor.author Clausen, David S.
dc.contributor.author Null, Ryan W.
dc.contributor.author Metzger, Bria M.
dc.contributor.author Willis, Amy D.
dc.contributor.author Ozpolat, B. Duygu
dc.date.accessioned 2022-01-24T21:35:48Z
dc.date.available 2022-01-24T21:35:48Z
dc.date.issued 2021-11-18
dc.description © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kuehn, E., Clausen, D. S., Null, R. W., Metzger, B. M., Willis, A. D., & Ozpolat, B. D. Segment number threshold determines juvenile onset of germline cluster expansion in Platynereis dumerilii. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, (2021.): 1-16, https://doi.org/10.1002/jez.b.23100. en_US
dc.description.abstract Development of sexual characters and generation of gametes are tightly coupled with growth. Platynereis dumerilii is a marine annelid that has been used to study germline development and gametogenesis. P. dumerilii has germ cell clusters found across the body in the juvenile worms, and the clusters eventually form the gametes. Like other segmented worms, P. dumerilii grows by adding new segments at its posterior end. The number of segments reflect the growth state of the worms and therefore is a useful and measurable growth state metric to study the growth-reproduction crosstalk. To understand how growth correlates with progression of gametogenesis, we investigated germline development across several developmental stages. We discovered a distinct transition period when worms increase the number of germline clusters at a particular segment number threshold. Additionally, we found that keeping worms short in segment number, by manipulating environmental conditions or via amputations, supported a segment number threshold requirement for germline development. Finally, we asked if these clusters in P. dumerilii play a role in regeneration (as similar free-roaming cells are observed in Hydra and planarian regeneration) and found that the clusters were not required for regeneration in P. dumerilii, suggesting a strictly germline nature. Overall, these molecular analyses suggest a previously unidentified developmental transition dependent on the growth state of juvenile P. dumerilii leading to substantially increased germline expansion. en_US
dc.description.sponsorship Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM138008 (to BDÖ) and R35GM133420 (to ADW) and Hibbitt Startup Funds (to BDÖ). en_US
dc.identifier.citation Kuehn, E., Clausen, D. S., Null, R. W., Metzger, B. M., Willis, A. D., & Ozpolat, B. D. (2021). Segment number threshold determines juvenile onset of germline cluster expansion in Platynereis dumerilii. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 1-16. en_US
dc.identifier.doi 10.1002/jez.b.23100
dc.identifier.uri https://hdl.handle.net/1912/27959
dc.publisher Wiley en_US
dc.relation.uri https://doi.org/10.1002/jez.b.23100
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Annelida en_US
dc.subject Critical size en_US
dc.subject Developmental transition en_US
dc.subject Gametogenesis en_US
dc.subject Sexual reproduction en_US
dc.title Segment number threshold determines juvenile onset of germline cluster expansion in Platynereis dumerilii en_US
dc.type Article en_US
dspace.entity.type Publication
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