(Elsevier, 2024-08-09)
Harry, Clayton J.; Hibshman, Jonathan D.; Damatac II, Amor; Davidson, Phillip L.; Estermann, Martin Andres; Flores-Flores, Marycruz; Holmes, Caroline M.; Lazaro, Jorge; Legere, Elizabeth Ann; Leyhr, Jake; Thendral, Siddharthan Balachandar; Vincent, Bridget A.; Goldstein, Bob
Tardigrades are microscopic organisms with exceptional resilience to environmental extremes. Most protocols to visualize the internal anatomy of tardigrades rely on fixation, hampering our understanding of dynamic changes to organelles and other subcellular components. Here, we provide protocols for staining live tardigrade adults and other postembryonic stages, facilitating real-time visualization of structures including lipid droplets, mitochondria, lysosomes, and DNA.
