Brown C. Titus

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Brown
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C. Titus
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Author: Brown, C. Titus × Author: Rosenthal, Joshua J. C. ×

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Cephalopod genomics : a plan of strategies and organization

2012-09-26 , Albertin, Caroline B. , Bonnaud, Laure , Brown, C. Titus , Crookes-Goodson, Wendy J. , da Fonseca, Rute R. , Di Cristo, Carlo , Dilkes, Brian P. , Edsinger-Gonzales, Eric , Freeman, Robert J. , Hanlon, Roger T. , Koenig, Kristen M. , Lindgren, Annie R. , Martindale, Mark Q. , Minx, Patrick , Moroz, Leonid L. , Nodl, Marie-Therese , Nyholm, Spencer V. , Ogura, Atsushi , Pungor, Judit R. , Rosenthal, Joshua J. C. , Schwarz, Erich M. , Shigeno, Shuichi , Strugnell, Jan M. , Wollesen, Tim , Zhang, Guojie , Ragsdale, Clifton W.

The Cephalopod Sequencing Consortium (CephSeq Consortium) was established at a NESCent Catalysis Group Meeting, “Paths to Cephalopod Genomics- Strategies, Choices, Organization,” held in Durham, North Carolina, USA on May 24-27, 2012. Twenty-eight participants representing nine countries (Austria, Australia, China, Denmark, France, Italy, Japan, Spain and the USA) met to address the pressing need for genome sequencing of cephalopod molluscs. This group, drawn from cephalopod biologists, neuroscientists, developmental and evolutionary biologists, materials scientists, bioinformaticians and researchers active in sequencing, assembling and annotating genomes, agreed on a set of cephalopod species of particular importance for initial sequencing and developed strategies and an organization (CephSeq Consortium) to promote this sequencing. The conclusions and recommendations of this meeting are described in this White Paper.

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Transcriptome of the Caribbean stony coral Porites astreoides from three developmental stages

2016-08-02 , Mansour, Tamer A. , Rosenthal, Joshua J. C. , Brown, C. Titus , Roberson, Loretta M.

Porites astreoides is a ubiquitous species of coral on modern Caribbean reefs that is resistant to increasing temperatures, overfishing, and other anthropogenic impacts that have threatened most other coral species. We assembled and annotated a transcriptome from this coral using Illumina sequences from three different developmental stages collected over several years: free-swimming larvae, newly settled larvae, and adults (>10 cm in diameter). This resource will aid understanding of coral calcification, larval settlement, and host–symbiont interactions. A de novo transcriptome for the P. astreoides holobiont (coral plus algal symbiont) was assembled using 594 Mbp of raw Illumina sequencing data generated from five age-specific cDNA libraries. The new transcriptome consists of 867 255 transcript elements with an average length of 685 bases. The isolated P. astreoides assembly consists of 129 718 transcript elements with an average length of 811 bases, and the isolated Symbiodinium sp. assembly had 186 177 transcript elements with an average length of 1105 bases. This contribution to coral transcriptome data provides a valuable resource for researchers studying the ontogeny of gene expression patterns within both the coral and its dinoflagellate symbiont.

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