Girguis Peter

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Last Name
Girguis
First Name
Peter
ORCID
0000-0002-3599-8160

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Now showing 1 - 20 of 32
  • Article
    A distinct and active bacterial community in cold oxygenated fluids circulating beneath the western flank of the Mid-Atlantic ridge
    (Nature Publishing Group, 2016-03-03) Meyer, Julie L. ; Jaekel, Ulrike ; Tully, Benjamin J. ; Glazer, Brian T. ; Wheat, C. Geoffrey ; Lin, Huei-Ting ; Hsieh, Chih-Chiang ; Cowen, James P. ; Hulme, Samuel M. ; Girguis, Peter R. ; Huber, Julie A.
    The rock-hosted, oceanic crustal aquifer is one of the largest ecosystems on Earth, yet little is known about its indigenous microorganisms. Here we provide the first phylogenetic and functional description of an active microbial community residing in the cold oxic crustal aquifer. Using subseafloor observatories, we recovered crustal fluids and found that the geochemical composition is similar to bottom seawater, as are cell abundances. However, based on relative abundances and functional potential of key bacterial groups, the crustal fluid microbial community is heterogeneous and markedly distinct from seawater. Potential rates of autotrophy and heterotrophy in the crust exceeded those of seawater, especially at elevated temperatures (25°C) and deeper in the crust. Together, these results reveal an active, distinct, and diverse bacterial community engaged in both heterotrophy and autotrophy in the oxygenated crustal aquifer, providing key insight into the role of microbial communities in the ubiquitous cold dark subseafloor biosphere. An Author Correction to this article was published on 16 April 2020
  • Article
    Links from mantle to microbe at the Lau Integrated Study Site : insights from a back-arc spreading center
    (The Oceanography Society, 2012-03) Tivey, Margaret K. ; Becker, Erin ; Beinart, Roxanne A. ; Fisher, Charles R. ; Girguis, Peter R. ; Langmuir, Charles H. ; Michael, Peter J. ; Reysenbach, Anna-Louise
    The Lau Integrated Study Site (ISS) has provided unique opportunities for study of ridge processes because of its back-arc setting in the southwestern Pacific. Its location allows study of a biogeographical province distinct from those of eastern Pacific and mid-Atlantic ridges, and crustal compositions along the ridge lie outside the range of mid-ocean ridge crustal compositions. The Lau ISS is located above a subduction zone, at an oblique angle. The underlying mantle receives water and other elements derived from the downgoing lithospheric slab, with an increase in slab influence from north to south. Water lowers the mantle melting temperature and leads to greater melt production where the water flux is greater, and to distinctive regional-scale gradients along the ridge. There are deeper faulted axial valleys with basaltic volcanism in the north and inflated axial highs with andesites in the south. Differences in igneous rock composition and release of magmatic volatiles affect compositions of vent fluids and deposits. Differences in vent fluid compositions and small-scale diffuse-flow regimes correlate with regional-scale patterns in microbial and megafaunal distributions. The interdisciplinary research effort at the Lau ISS has successfully identified linkages between subsurface processes and deep-sea biological communities, from mantle to microbe to megafauna.
  • Article
    CRISPR/Cas9-induced disruption of Bodo saltans paraflagellar rod-2 gene reveals its importance for cell survival
    (Society for Applied Microbiology, 2022-01-31) Gomaa, Fatma ; Li, Zhu-Hong ; Beaudoin, David J. ; Alzan, Heba ; Girguis, Peter R. ; Docampo, Roberto ; Edgcomb, Virginia P.
    Developing transfection protocols for marine protists is an emerging field that will allow the functional characterization of protist genes and their roles in organism responses to the environment. We developed a CRISPR/Cas9 editing protocol for Bodo saltans, a free-living kinetoplastid with tolerance to both marine and freshwater conditions and a close non-parasitic relative of trypanosomatids. Our results show that SaCas9/single-guide RNA (sgRNA) ribonucleoprotein (RNP) complex-mediated disruption of the paraflagellar rod 2 gene (BsPFR2) was achieved using electroporation-mediated transfection. The use of CRISPR/Cas9 genome editing can increase the efficiency of targeted homologous recombination when a repair DNA template is provided. Our sequence analysis suggests two mechanisms for repairing double-strand breaks in B. saltans are active; homologous-directed repair (HDR) utilizing an exogenous DNA template that carries an antibiotic resistance gene and likley non-homologous end joining (NHEJ). However, HDR was only achieved when a single (vs. multiple) SaCas9 RNP complex was provided. Furthermore, the biallelic knockout of BsPFR2 was detrimental for the cell, highlighting its essential role for cell survival because it facilitates the movement of food particles into the cytostome. Our Cas9/sgRNA RNP complex protocol provides a new tool for assessing gene functions in B. saltans and perhaps similar protists with polycistronic transcription.
  • Dataset
    Fe(II) concentrations over time in iron oxidizing bacteria cultures.
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-03-26) Girguis, Peter ; Emerson, David
    Fe(II) concentrations over time in iron oxidizing bacteria cultures. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/709440
  • Dataset
    Sulfide concentrations as a function of time from INSPIRE track 1 collected from 2013 to 2017 (INSPIRE Pyrite project)
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-06-11) Girguis, Peter ; Clarke, David
    Sulfide concentrations as a function of time from INSPIRE track 1 collected from 2013 to 2017 (INSPIRE Pyrite project) For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/684417
  • Dataset
    Chemistry and cell counts of formation fluids from North Pond, western flank of the Mid-Atlantic Ridge, from 2012-2014
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-12-02) Huber, Julie ; Girguis, Peter ; Glazer, Brian
    Chemistry and cell counts of formation fluids from North Pond For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/782025
  • Dataset
    Oxygen consumption rates/zero valent iron dissolution of FeOB with kanamycin addition - replacement samples
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-03-26) Girguis, Peter ; Emerson, David
    Oxygen consumption rates/zero valent iron dissolution of FeOB with kanamycin addition - replacement samples For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/709573
  • Dataset
    Concentrations, d13C and D14C data for DOC and DIC in fluids collected from North Pond Cork Observatories U1382A and U1383C and from bottom seawater in 2012, 2014 and 2017.
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-08-10) Girguis, Peter ; Shah Walter, Sunita R.
    Concentrations, d13C and D14C data for DOC and DIC in fluids collected from North Pond Cork Observatories U1382A and U1383C and from bottom seawater in 2012, 2014 and 2017. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/876729
  • Dataset
    Discovering hydrothermalism from afar: in situ methane instrumentation and change-point detection for decision-making
    (Woods Hole Oceanographic Institution, 2022-10-06) Michel, Anna P. M. ; Wankel, Scott D. ; Preston, Victoria Lynn ; Flaspohler, Genevieve Elaine ; Kapit, Jason ; Pardis, William A. ; Youngs, Sarah ; Martocello, Donald E. ; Girguis, Peter R. ; Roy, Nicholas
    Seafloor hydrothermalism plays a critical role in fundamental interactions between geochemical and biological processes in the deep ocean. A significant number of hydrothermal vents are hypothesized to exist, but many of these remain undiscovered due in part to the difficulty of detecting hydrothermalism using standard sensors on rosettes towed in the water column or robotic platforms performing surveys. Here, we use in situ methane sensors to complement standard sensing technology for hydrothermalism discovery and compare sensing equipment on a towed rosette and autonomous underwater vehicle (AUV) during a 17 km long transect in the Northern Guaymas Basin. This transect spatially intersected with a known hydrothermally active venting site. These data show that methane signaled possible hydrothermal activity 1.5-3 km laterally (100-150m vertically) from a known vent. Methane as a signal for hydrothermalism performed similarly to standard turbidity sensors (plume detection 2.2-3.3 km from reference source), and more sensitively and clearly than temperature, salinity, and oxygen instruments which readily respond to physical mixing in background seawater. We additionally introduce change-point detection algorithms---streaming cross-correlation and regime identification---as a means of real-time hydrothermalism discovery and discuss related data monitoring technologies that could be used in planning, executing, and monitoring explorative surveys for hydrothermalism.
  • Dataset
    X-ray diffractogram data collected from 2013 to 2017 (INSPIRE Pyrite project)
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-04-03) Girguis, Peter ; Clarke, David
    X-ray diffractogram data collected from 2013 to 2017 (INSPIRE Pyrite project) For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/684634
  • Article
    Discovering hydrothermalism from afar: In Situ methane instrumentation and change-point detection for decision-making
    (Frontiers Media, 2022-10-25) Preston, Victoria Lynn ; Flaspohler, Genevieve Elaine ; Kapit, Jason ; Pardis, William A. ; Youngs, Sarah ; Martocello, Donald E., III ; Roy, Nicholas ; Girguis, Peter R. ; Wankel, Scott ; Michel, Anna P. M.
    Seafloor hydrothermalism plays a critical role in fundamental interactions between geochemical and biological processes in the deep ocean. A significant number of hydrothermal vents are hypothesized to exist, but many of these remain undiscovered due in part to the difficulty of detecting hydrothermalism using standard sensors on rosettes towed in the water column or robotic platforms performing surveys. Here, we use in situ methane sensors to complement standard sensing technology for hydrothermalism discovery and compare sensors on a towed rosette and an autonomous underwater vehicle (AUV) during a 17 km long transect in the Northern Guaymas Basin in the Gulf of California. This transect spatially intersected with a known hydrothermally active venting site. These data show that methane signalled possible hydrothermal-activity 1.5–3 km laterally (100–150 m vertically) from a known vent. Methane as a signal for hydrothermalism performed similarly to standard turbidity sensors (plume detection 2.2–3.3 km from reference source), and more sensitively and clearly than temperature, salinity, and oxygen instruments which readily respond to physical mixing in background seawater. We additionally introduce change-point detection algorithms—streaming cross-correlation and regime identification—as a means of real-time hydrothermalism discovery and discuss related data supervision technologies that could be used in planning, executing, and monitoring explorative surveys for hydrothermalism.
  • Dataset
    Pore water and solid phase iron geochemical data from a coastal Maine intertidal mudflat from November 2015 to November 2016
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-03-15) Emerson, David ; Girguis, Peter
    Pore water and solid phase iron geochemical data from a coastal Maine intertidal mudflat from November 2015 to November 2016. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/737962
  • Dataset
    Illumina sequencing data from sediment strata collected from the cold seeps of Hydrate Ridge, metalliferous sediments of Juan de Fuca Ridge, and organic-rich hydrothermal sediments of Guaymas Basin
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-03-15) Girguis, Peter
    Illumina sequencing data (NCBI accession numbers) from sediment strata collected from the cold seeps of Hydrate Ridge, metalliferous sediments of Juan de Fuca Ridge, and organic-rich hydrothermal sediments of Guaymas Basin. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/747948
  • Dataset
    XRD, XPS, and raman data collected from 2013 to 2017 (INSPIRE Pyrite)
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-04-03) Girguis, Peter ; Clarke, David
    XRD, XPS, and raman data collected from 2013 to 2017 (INSPIRE Pyrite) For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/684649
  • Dataset
    The acetate and inorganic carbon uptake rates as determined via stable isotopic tracers from Maria S. Merian cruise MSM20-5 in 2012; data generated using the formation fluids recovered from CORKs installed at North Pond
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-05-13) Girguis, Peter ; Glazer, Brian ; Huber, Julie
    This dataset includes the acetate and inorganic carbon uptake rates as determined via stable isotopic tracers from Maria S. Merian cruise MSM20-5 in 2012; data were generated using the formation fluids recovered from CORKs installed at North Pond. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/637804
  • Dataset
    Particulate organic carbon from the formation fluids recovered from the CORKs installed at North Pond; collected on Maria S. Merian cruise MSM20-5 in 2012
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-05-13) Girguis, Peter ; Glazer, Brian ; Huber, Julie
    This dataset includes particulate organic carbon from the formation fluids recovered from the CORKs installed at North Pond; collected on Maria S. Merian cruise MSM20-5 in 2012. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/630335
  • Dataset
    Sulfate reduction energetics at Main Endeavor grotto chimney from samples collected on RV Atlantis (AT18-08) during Jason II dives in the Juan de Fuca Ridge from July to August 2011
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-04-22) Girguis, Peter ; Rogers, Karyn
    Sulfate reduction energetics at Main Endeavor grotto chimney from samples collected on RV Atlantis (AT18-08) during Jason II dives in the Juan de Fuca Ridge from July to August 2011 For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/661659
  • Dataset
    Net oxygen consumption rates of the fluids recovered from the CORKs installed at North Pond; collected on Maria S. Merian cruise MSM37 in 2014
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-05-13) Girguis, Peter ; Glazer, Brian ; Huber, Julie
    This dataset includes net oxygen consumption rates of the fluids recovered from the CORKs installed at North Pond; collected on Maria S. Merian cruise MSM37 in 2014. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/630314
  • Article
    Exploration of the Northern Guaymas Basin
    (The Oceanography Society, 2018-03) Soule, Samuel A. ; Seewald, Jeffrey S. ; Wankel, Scott D. ; Michel, Anna P. M. ; Beinart, Roxanne A. ; Escobar Briones, Elva ; Morales Dominguez, Esmerelda ; Girguis, Peter R. ; Coleman, Dwight ; Raineault, Nicole A. ; Wagner, Jamie K.S. ; Foulk, Aubrey ; Bagla, Anshika ; Karson, Jeffrey A.
  • Article
    Physiological dynamics of chemosynthetic symbionts in hydrothermal vent snails
    (Springer Nature, 2020-07-02) Breusing, Corinna ; Mitchell, Jessica ; Delaney, Jennifer ; Sylva, Sean P. ; Seewald, Jeffrey S. ; Girguis, Peter R. ; Beinart, Roxanne A.
    Symbioses between invertebrate animals and chemosynthetic bacteria form the basis of hydrothermal vent ecosystems worldwide. In the Lau Basin, deep-sea vent snails of the genus Alviniconcha associate with either Gammaproteobacteria (A. kojimai, A. strummeri) or Campylobacteria (A. boucheti) that use sulfide and/or hydrogen as energy sources. While the A. boucheti host–symbiont combination (holobiont) dominates at vents with higher concentrations of sulfide and hydrogen, the A. kojimai and A. strummeri holobionts are more abundant at sites with lower concentrations of these reductants. We posit that adaptive differences in symbiont physiology and gene regulation might influence the observed niche partitioning between host taxa. To test this hypothesis, we used high-pressure respirometers to measure symbiont metabolic rates and examine changes in gene expression among holobionts exposed to in situ concentrations of hydrogen (H2: ~25 µM) or hydrogen sulfide (H2S: ~120 µM). The campylobacterial symbiont exhibited the lowest rate of H2S oxidation but the highest rate of H2 oxidation, with fewer transcriptional changes and less carbon fixation relative to the gammaproteobacterial symbionts under each experimental condition. These data reveal potential physiological adaptations among symbiont types, which may account for the observed net differences in metabolic activity and contribute to the observed niche segregation among holobionts.