(RSC Publishing, 2012-11-29)
Krasitskaya, Vasilisa V.; Kudryavtsev, Alexander N.; Shimomura, Osamu; Frank, Ludmila A.
Two obelin mutants distinctly different in the color of bioluminescence were successfully applied to
simultaneous detection of two analytes in a single well. The flash-type signals were triggered by single
injection of Ca2+ and were discriminated by the spectral and time resolutions. The technique was
developed in a high-throughput format, and applied to simultaneous immunoassay of two
gonadotropic hormones – luteinizing (lutropin or hLH) and follicle stimulating (hFSH) – in clinical sera,
and to simultaneous detection of two gene allelic variants at single nucleotide polymorphism
genotyping of the human F5 gene encoding factor V Leiden polymorphism 1691 G/A (R506Q). A
considerable number of samples were investigated and the results obtained were in good correlation
with those obtained by using traditional techniques.
