Ozpolat B. Duygu

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Last Name
Ozpolat
First Name
B. Duygu
ORCID
0000-0002-1900-965X

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Author: Handberg-Thorsager, Mette ×

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  • Article
    Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelid Platynereis dumerilii
    (eLife, 2017-12-12) Ozpolat, B. Duygu ; Handberg-Thorsager, Mette ; Vervoort, Michel ; Balavoine, Guillaume
    Cell lineage, cell cycle, and cell fate are tightly associated in developmental processes, but in vivo studies at single-cell resolution showing the intricacies of these associations are rare due to technical limitations. In this study on the marine annelid Platynereis dumerilii, we investigated the lineage of the 4d micromere, using high-resolution long-term live imaging complemented with a live-cell cycle reporter. 4d is the origin of mesodermal lineages and the germline in many spiralians. We traced lineages at single-cell resolution within 4d and demonstrate that embryonic segmental mesoderm forms via teloblastic divisions, as in clitellate annelids. We also identified the precise cellular origins of the larval mesodermal posterior growth zone. We found that differentially-fated progeny of 4d (germline, segmental mesoderm, growth zone) display significantly different cell cycling. This work has evolutionary implications, sets up the foundation for functional studies in annelid stem cells, and presents newly established techniques for live imaging marine embryos.
  • Article
    The Nereid on the rise: Platynereis as a model system
    (BMC, 2021-09-27) Ozpolat, B. Duygu ; Randel, Nadine ; Williams, Elizabeth A. ; Bezares-Calderón, Luis Alberto ; Andreatta, Gabriele ; Balavoine, Guillaume ; Bertucci, Paola Y. ; Ferrier, David E. K. ; Gambi, Maria Cristina ; Gazave, Eve ; Handberg-Thorsager, Mette ; Hardege, Jörg ; Hird, Cameron ; Hsieh, Yu-Wen ; Hui, Jerome ; Mutemi, Kevin Nzumbi ; Schneider, Stephan Q. ; Simakov, Oleg ; Vergara, Hernando M. ; Vervoort, Michel ; Jékely, Gáspár ; Tessmar-Raible, Kristin ; Raible, Florian ; Arendt, Detlev
    The Nereid Platynereis dumerilii (Audouin and Milne Edwards (Annales des Sciences Naturelles 1:195–269, 1833) is a marine annelid that belongs to the Nereididae, a family of errant polychaete worms. The Nereid shows a pelago-benthic life cycle: as a general characteristic for the superphylum of Lophotrochozoa/Spiralia, it has spirally cleaving embryos developing into swimming trochophore larvae. The larvae then metamorphose into benthic worms living in self-spun tubes on macroalgae. Platynereis is used as a model for genetics, regeneration, reproduction biology, development, evolution, chronobiology, neurobiology, ecology, ecotoxicology, and most recently also for connectomics and single-cell genomics. Research on the Nereid started with studies on eye development and spiralian embryogenesis in the nineteenth and early twentieth centuries. Transitioning into the molecular era, Platynereis research focused on posterior growth and regeneration, neuroendocrinology, circadian and lunar cycles, fertilization, and oocyte maturation. Other work covered segmentation, photoreceptors and other sensory cells, nephridia, and population dynamics. Most recently, the unique advantages of the Nereid young worm for whole-body volume electron microscopy and single-cell sequencing became apparent, enabling the tracing of all neurons in its rope-ladder-like central nervous system, and the construction of multimodal cellular atlases. Here, we provide an overview of current topics and methodologies for P. dumerilii, with the aim of stimulating further interest into our unique model and expanding the active and vibrant Platynereis community.