Mydlarz Laura

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Mydlarz
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Laura
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  • Article
    Alphaflexivirus genomes in stony coral tissue loss disease-affected, disease-exposed, and disease-unexposed coral colonies in the U.S. Virgin Islands
    (American Society for Microbiology, 2022-02-17) Veglia, Alex J. ; Beavers, Kelsey ; Van Buren, Emily W. ; Meiling, Sonora S. ; Muller, Erinn ; Smith, Tyler B. ; Holstein, Daniel M. ; Apprill, Amy ; Brandt, Marilyn ; Mydlarz, Laura ; Correa, Adrienne M.S.
    Stony coral tissue loss disease (SCTLD) is decimating Caribbean corals. Here, through the metatranscriptomic assembly and annotation of two alphaflexivirus-like strains, we provide genomic evidence of filamentous viruses in SCTLD-affected, -exposed, and -unexposed coral colonies. These data will assist in clarifying the roles of viruses in SCTLD.
  • Dataset
    Phenotypic information collected from white plague disease exposure in a controlled environment at The University of the Virgin Islands Center for Marine and Environmental Studies in June of 2017
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-18) Mydlarz, Laura ; Brandt, Marilyn ; MacKnight, Nicholas
    Phenotypic information collected from white plague disease exposure in a controlled environment at The University of the Virgin Islands Center for Marine and Environmental Studies in June of 2017. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829113
  • Dataset
    RNAseq data used to identify Alphaflexivirus genomes in Stony Coral Tissue Loss Disease-affected, disease-exposed, and disease-unexposed coral colonies in the U.S. Virgin Islands (Multi-Species Coral Disease project)
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-06-14) Mydlarz, Laura ; Correa, Adrienne M.S.
    RNA-seq data from healthy, SCTLD-exposed, and SCTLD-infected coral samples taken from a transmission experiment carried out in the US Virgin Islands. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/875583
  • Dataset
    Annual health surveys of the sea fan Gorgonia ventalina in La Parguera, Puerto Rico from 2006-2010
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-03-01) Harvell, Drew ; Mydlarz, Laura
    Health surveys of the sea fan Gorgonia ventalina were carried out from 2006 to 2010 at two coral reefs in La Parguera, Puerto Rico. The proportions of healthy and abnormal G. ventalina colonies along survey transects are reported. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/3720
  • Dataset
    Ecological results of SCTLD multi-species transmission experiment at the University of the Virgin Islands Center for Marine and Environmental Studies
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-07-20) Brandt, Marilyn ; Correa, Adrienne M.S. ; Meiling, Sonora ; Veglia, Alex J. ; Lasseigne, Danielle ; MacKnight, Nicholas ; Dimos, Bradford ; Huntley, Naomi ; Muller, Erinn ; Mydlarz, Laura ; Apprill, Amy ; Smith, Tyler ; Holstein, Daniel
    This dataset represents the ecological results of a stony coral tissue loss disease (SCTLD) multi-species transmission experiment. Eight colonies of six species of corals (Colpophyllia natans, Montastraea cavernosa, Orbicella annularis, Porites astreoides, Pseudodiploria strigosa, and Siderastrea siderea) were split in half and one half of these fragments were simultaneously exposed toSCTLD-affected colonies of Diploria labyrinthiformis and the other half were exposed to healthy colonies of D. labyrinthiformis. All corals were monitored for lesion appearance over an eight day experimental period. No lesions were recorded on healthy-exposed corals. Numbers of fragments showing lesion appearance, time to lesion appearance, and expansion rates of lesions for SCTLD-exposed corals are reported here. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/875156
  • Dataset
    Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient conducted at the Harvell lab at Cornell University
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-03-01) Harvell, Drew ; Mydlarz, Laura
    Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient. Two types of assays were used in two trials to quantify Labyrinthulomycota cultures: cell counts using a hemocytometer and total protein concentration. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/3719
  • Article
    Variable species responses to experimental stony coral tissue loss disease (SCTLD) exposure
    (Frontiers Media, 2021-04-30) Meiling, Sonora S. ; Muller, Erinn ; Lasseigne, Danielle ; Rossin, Ashley ; Veglia, Alex J. ; MacKnight, Nicholas ; Dimos, Bradford ; Huntley, Naomi ; Correa, Adrienne M. S. ; Smith, Tyler B. ; Holstein, Daniel M. ; Mydlarz, Laura ; Apprill, Amy ; Brandt, Marilyn
    Stony coral tissue loss disease (SCTLD) was initially documented in Florida in 2014 and outbreaks with similar characteristics have since appeared in disparate areas throughout the northern Caribbean, causing significant declines in coral communities. SCTLD is characterized by focal or multifocal lesions of denuded skeleton caused by rapid tissue loss and affects at least 22 reef-building species of Caribbean corals. A tissue-loss disease consistent with the case definition of SCTLD was first observed in the U.S. Virgin Islands (USVI) in January of 2019 off the south shore of St. Thomas at Flat Cay. The objective of the present study was to characterize species susceptibility to the disease present in St. Thomas in a controlled laboratory transmission experiment. Fragments of six species of corals (Colpophyllia natans, Montastraea cavernosa, Orbicella annularis, Porites astreoides, Pseudodiploria strigosa, and Siderastrea siderea) were simultaneously incubated with (but did not physically contact) SCTLD-affected colonies of Diploria labyrinthiformis and monitored for lesion appearance over an 8 day experimental period. Paired fragments from each corresponding coral genotype were equivalently exposed to apparently healthy colonies of D. labyrinthiformis to serve as controls; none of these fragments developed lesions throughout the experiment. When tissue-loss lesions appeared and progressed in a disease treatment, the affected coral fragment, and its corresponding control genet, were removed and preserved for future analysis. Based on measures including disease prevalence and incidence, relative risk of lesion development, and lesion progression rates, O. annularis, C. natans, and S. siderea showed the greatest susceptibility to SCTLD in the USVI. These species exhibited earlier average development of lesions, higher relative risk of lesion development, greater lesion prevalence, and faster lesion progression rates compared with the other species, some of which are considered to be more susceptible based on field observations (e.g., P. strigosa). The average transmission rate in the present study was comparable to tank studies in Florida, even though disease donor species differed. Our findings suggest that the tissue loss disease affecting reefs of the USVI has a similar epizootiology to that observed in other regions, particularly Florida.
  • Dataset
    NCBI accession numbers for RNAseq data from five coral species experimentally exposed to Stony Coral Tissue Loss Disease (SCTLD) at the University of the Virgin Islands in 2019
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-10-05) Mydlarz, Laura
    Raw RNA sequence data were obtained from a disease transmission experiment carried out at the University of the Virgin Islands in which five reef-building coral species, Colpophyllia natans, Orbicella annularis, Pseudodiploria strigosa, Porites astreoides, and Montastraea cavernosa, were exposed to Stony Coral Tissue Loss Disease (SCTLD) in mesocosms. Sequences were used to compare the differential expression of host and endosymbiont genes between disease states (control, disease-exposed, and disease-infected) and to elucidate a transcriptomic model of the holobiont response to SCTLD. This dataset includes National Center for Biotechnology Information (NCBI) accession numbers and related data for those five coral species examined in the experiment. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/881776
  • Article
    Experimental transmission of Stony Coral Tissue Loss Disease results in differential microbial responses within coral mucus and tissue
    (Springer, 2022-05-30) Huntley, Naomi ; Brandt, Marilyn ; Becker, Cynthia ; Miller, Carolyn A. ; Meiling, Sonora S. ; Correa, Adrienne M.S. ; Holstein, Daniel M. ; Muller, Erinn ; Mydlarz, Laura ; Smith, Tyler B. ; Apprill, Amy
    Stony coral tissue loss disease (SCTLD) is a widespread and deadly disease that affects nearly half of Caribbean coral species. To understand the microbial community response to this disease, we performed a disease transmission experiment on US Virgin Island (USVI) corals, exposing six species of coral with varying susceptibility to SCTLD. The microbial community of the surface mucus and tissue layers were examined separately using a small subunit ribosomal RNA gene-based sequencing approach, and data were analyzed to identify microbial community shifts following disease acquisition, potential causative pathogens, as well as compare microbiota composition to field-based corals from the USVI and Florida outbreaks. While all species displayed similar microbiome composition with disease acquisition, microbiome similarity patterns differed by both species and mucus or tissue microhabitat. Further, disease exposed but not lesioned corals harbored a mucus microbial community similar to those showing disease signs, suggesting that mucus may serve as an early warning detection for the onset of SCTLD. Like other SCTLD studies in Florida, Rhodobacteraceae, Arcobacteraceae, Desulfovibrionaceae, Peptostreptococcaceae, Fusibacter, Marinifilaceae, and Vibrionaceae dominated diseased corals. This study demonstrates the differential response of the mucus and tissue microorganisms to SCTLD and suggests that mucus microorganisms may be diagnostic for early disease exposure.
  • Dataset
    Viral consortia in Stony Coral Tissue Loss Disease- affected, disease-exposed, and disease-unexposed coral colonies from a transmission experiment conducted on samples collected from Rupert’s Rock in St. Thomas, U.S. Virgin Islands in 2019
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-11-04) Veglia, Alex J. ; Beavers, Kelsey ; Mydlarz, Laura ; Correa, Adrienne M.S.
    To understand the extent to which (if any) viruses are associated with stony coral tissue loss disease (SCTLD) in stony corals of the U.S. Virgin Islands, we leveraged viral metatranscriptomes generated from SCTLD-affected, SCTLD-exposed, and control (unexposed) coral holobionts sampled during a SCTLD transmission experiment. Sequence data is available in NCBI Genbank under BioProject accession PRJNA788911. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/875283
  • Article
    Stony coral tissue loss disease induces transcriptional signatures of in situ degradation of dysfunctional Symbiodiniaceae
    (Nature Research, 2023-05-22) Beavers, Kelsey M. ; Van Buren, Emily W. ; Rossin, Ashley M. ; Emery, Madison A. ; Veglia, Alex J. ; Karrick, Carly E. ; MacKnight, Nicholas J. ; Dimos, Bradford A. ; Meiling, Sonora S. ; Smith, Tyler B. ; Apprill, Amy ; Muller, Erinn M. ; Holstein, Daniel M. ; Correa, Adrienne M. S. ; Brandt, Marilyn E. ; Mydlarz, Laura D.
    Stony coral tissue loss disease (SCTLD), one of the most pervasive and virulent coral diseases on record, affects over 22 species of reef-building coral and is decimating reefs throughout the Caribbean. To understand how different coral species and their algal symbionts (family Symbiodiniaceae) respond to this disease, we examine the gene expression profiles of colonies of five species of coral from a SCTLD transmission experiment. The included species vary in their purported susceptibilities to SCTLD, and we use this to inform gene expression analyses of both the coral animal and their Symbiodiniaceae. We identify orthologous coral genes exhibiting lineage-specific differences in expression that correlate to disease susceptibility, as well as genes that are differentially expressed in all coral species in response to SCTLD infection. We find that SCTLD infection induces increased expression of rab7, an established marker of in situ degradation of dysfunctional Symbiodiniaceae, in all coral species accompanied by genus-level shifts in Symbiodiniaceae photosystem and metabolism gene expression. Overall, our results indicate that SCTLD infection induces symbiophagy across coral species and that the severity of disease is influenced by Symbiodiniaceae identity.