Bastoni Deborah

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Bastoni
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Deborah
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  • Article
    Assessment of spatio-temporal variability of faecal pollution along coastal waters during and after rainfall events
    (MDPI, 2022-02-08) Manini, Elena ; Baldrighi, Elisa ; Ricci, Fabio ; Grilli, Federica ; Giovannelli, Donato ; Intoccia, Michele ; Casabianca, Silvia ; Capellacci, Samuela ; Marinchel, Nadia ; Penna, Pierluigi ; Moro, Fabrizio ; Campanelli, Alessandra ; Cordone, Angelina ; Correggia, Monica ; Bastoni, Deborah ; Bolognini, Luigi ; Marini, Mauro ; Penna, Antonella
    More than 80% of wastewaters are discharged into rivers or seas, with a negative impact on water quality along the coast due to the presence of potential pathogens of faecal origin. Escherichia coli and enterococci are important indicators to assess, monitor, and predict microbial water quality in natural ecosystems. During rainfall events, the amount of wastewater delivered to rivers and coastal systems is increased dramatically. This study implements measures capable of monitoring the pathways of wastewater discharge to rivers and the transport of faecal bacteria to the coastal area during and following extreme rainfall events. Spatio-temporal variability of faecal microorganisms and their relationship with environmental variables and sewage outflow in an area located in the western Adriatic coast (Fano, Italy) was monitored. The daily monitoring during the rainy events was carried out for two summer seasons, for a total of five sampling periods. These results highlight that faecal microbial contaminations were related to rainy events with a high flow of wastewater, with recovery times for the microbiological indicators varying between 24 and 72 h and influenced by a dynamic dispersion. The positive correlation between ammonium and faecal bacteria at the Arzilla River and the consequences in seawater can provide a theoretical basis for controlling ammonium levels in rivers as a proxy to monitor the potential risk of bathing waters pathogen pollution.
  • Article
    Surface bacterioplankton community structure crossing the Antarctic Circumpolar Current Fronts
    (MDPI, 2023-03-09) Cordone, Angelina ; Selci, Matteo ; Barosa, Bernardo ; Bastianoni, Alessia ; Bastoni, Deborah ; Bolinesi, Francesco ; Capuozzo, Rosaria ; Cascone, Martina ; Correggia, Monica ; Corso, Davide ; Di Iorio, Luciano ; Misic, Cristina ; Montemagno, Francesco ; Ricciardelli, Annarita ; Saggiomo, Maria ; Tonietti, Luca ; Mangoni, Olga ; Giovannelli, Donato
    The Antarctic Circumpolar Current (ACC) is the major current in the Southern Ocean, isolating the warm stratified subtropical waters from the more homogeneous cold polar waters. The ACC flows from west to east around Antarctica and generates an overturning circulation by fostering deep-cold water upwelling and the formation of new water masses, thus affecting the Earth's heat balance and the global distribution of carbon. The ACC is characterized by several water mass boundaries or fronts, known as the Subtropical Front (STF), Subantarctic Front (SAF), Polar Front (PF), and South Antarctic Circumpolar Current Front (SACCF), identified by typical physical and chemical properties. While the physical characteristics of these fronts have been characterized, there is still poor information regarding the microbial diversity of this area. Here we present the surface water bacterioplankton community structure based on 16S rRNA sequencing from 13 stations sampled in 2017 between New Zealand to the Ross Sea crossing the ACC Fronts. Our results show a distinct succession in the dominant bacterial phylotypes present in the different water masses and suggest a strong role of sea surface temperatures and the availability of Carbon and Nitrogen in controlling community composition. This work represents an important baseline for future studies on the response of Southern Ocean epipelagic microbial communities to climate change.
  • Article
    Oxidoreductases and metal cofactors in the functioning of the earth
    (Biochemical Society, 2023-08-11) Mele, Bruno Hay ; Monticelli, Maria ; Leone, Serena ; Bastoni, Deborah ; Barosa, Bernardo ; Cascone, Martina ; Migliaccio, Flavia ; Montemagno, Francesco ; Ricciardelli, Annarita ; Tonietti, Luca ; Rotundi, Alessandra ; Cordone, Angelina ; Giovannelli, Donato
    Life sustains itself using energy generated by thermodynamic disequilibria, commonly existing as redox disequilibria. Metals are significant players in controlling redox reactions, as they are essential components of the engine that life uses to tap into the thermodynamic disequilibria necessary for metabolism. The number of proteins that evolved to catalyze redox reactions is extraordinary, as is the diversification level of metal cofactors and catalytic domain structures involved. Notwithstanding the importance of the topic, the relationship between metals and the redox reactions they are involved in has been poorly explored. This work reviews the structure and function of different prokaryotic organometallic–protein complexes, highlighting their pivotal role in controlling biogeochemistry. We focus on a specific subset of metal-containing oxidoreductases (EC1 or EC7.1), which are directly involved in biogeochemical cycles, i.e., at least one substrate or product is a small inorganic molecule that is or can be exchanged with the environment. Based on these inclusion criteria, we select and report 59 metalloenzymes, describing the organometallic structure of their active sites, the redox reactions in which they are involved, and their biogeochemical roles.
  • Article
    Shark microbiome analysis demonstrates unique microbial communities in two distinct Mediterranean Sea shark species
    (MDPI, 2024-03-11) Montemagno, Francesco ; Romano, Chiara ; Bastoni, Deborah ; Cordone, Angelina ; De Castro, Olga ; Stefanni, Sergio ; Sperone, Emilio ; Giovannelli, Donato
    Our knowledge regarding the role of the microbiome in fish health has been steadily increasing in the last decade, especially for species of commercial interest. Conversely, relatively few studies focus on the microbiomes of wild fish, especially apex predators like sharks, due to lower economic interest and greater difficulty in obtaining samples. Studies investigating microbiome differences between diverse anatomical locations of sharks are limited, and the majority of the available studies are focused on the microbial diversity present on shark teeth, with the aim of preventing infections due to bites of these animals or evaluating the presence of certain pathogens in healthy or diseased specimens. Here, we investigated the skin, mouth, gills, and cloaca microbiomes of five individuals of two phylogenetically distant species of sharks (Prionace glauca and Somniosus rostratus) to obtain a better understanding of the diversity regarding the microbiomes of these animals, how they change throughout different body parts, and how much they are influenced and determined by the ecology and evolutionary relationship between host and microbiome. To confirm the taxonomy of the sharks under study, we barcoded the specimens by sequencing the mtDNA COI from a biopsy of their skin. Microbial diversity based on the 16S rRNA gene reveals that partially overlapping microbiomes inhabit different body parts of each shark species, while the communities are distinct between the two species. Our results suggest that sharks’ microbiome species-specific differences are controlled by the ecology of the shark species. This is the first study comparatively analyzing the microbiome diversity of different anatomical locations in two shark species of the Mediterranean Sea.
  • Article
    Subsurface microbial community structure shifts along the geological features of the Central American Volcanic Arc
    (Public Library of Science, 2024-11-13) Basili, Marco ; Rogers, Timothy J. ; Nakagawa, Mayuko ; Yücel, Mustafa ; de Moor, J. Maarten ; Barry, Peter H. ; Schrenk, Matthew O. ; Jessen, Gerdhard L. ; Sánchez-Murillo, Ricardo ; Zahirovic, Sabin ; Bekaert, David V. ; Ramirez, Carlos J. ; Bastoni, Deborah ; Cordone, Angelina ; Lloyd, Karen G. ; Giovannelli, Donato
    Subduction of the Cocos and Nazca oceanic plates beneath the Caribbean plate drives the upward movement of deep fluids enriched in carbon, nitrogen, sulfur, and iron along the Central American Volcanic Arc (CAVA). These compounds fuel diverse subsurface microbial communities that in turn alter the distribution, redox state, and isotopic composition of these compounds. Microbial community structure and functions vary according to deep fluid delivery across the arc, but less is known about how microbial communities differ along the axis of a convergent margin as geological features (e.g., extent of volcanism and subduction geometry) shift. Here, we investigate changes in bacterial 16S rRNA gene amplicons and geochemical analysis of deeply-sourced seeps along the southern CAVA, where subduction of the Cocos Ridge alters the geological setting. We find shifts in community composition along the convergent margin, with communities in similar geological settings clustering together independently of the proximity of sample sites. Microbial community composition correlates with geological variables such as host rock type, maturity of hydrothermal fluid and slab depth along different segments of the CAVA. This reveals tight coupling between deep Earth processes and subsurface microbial activity, controlling community distribution, structure and composition along a convergent margin.