(BioMed Central Ltd, 2010-12-08)
Adey, Andrew; Morrison, Hilary G.; Asan; Xun, Xu; Kitzman, Jacob O.; Turner, Emily H.; Stackhouse, Bethany; MacKenzie, Alexandra P.; Caruccio, Nicholas C.; Zhang, Xiuqing; Shendure, Jay
We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.
