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  • Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition 

    Adey, Andrew; Morrison, Hilary G.; Asan; Xun, Xu; Kitzman, Jacob O.; Turner, Emily H.; Stackhouse, Bethany; MacKenzie, Alexandra P.; Caruccio, Nicholas C.; Zhang, Xiuqing; Shendure, Jay (BioMed Central Ltd, 2010-12-08)
    We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to ...