http://lod.bco-dmo.org/id/dataset/641479
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-03-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Calcification Rates and Biomass of 4 Coral Species, 2 Temperatures and 2 pCO2 Levels from Experiments at LTER site in Moorea, French Polynesia, 2011 (OA_Corals project)
2016-04-04
publication
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2016-04-05
publication
http://dx.doi.org/10.1575/1912/bco-dmo.641945
Dr Peter J Edmunds
California State University Northridge
principalInvestigator
documentDigital
Coral biomass and calicification rate at 2 temperatures and 2 pCO2 levels Dataset Description: <p>Area-normalized calcification (mg cm-2 d-1) and biomass normalized calcification (mg mg-1) for <em>Pocillopora meandrina</em>, massive <em>Porites </em>spp., <em>Acropora pulchra</em> and <em>Millepora platyphylla</em>, as a function of pCO2 (408 µatm versus 913 µatm) and temperature (28.0°C and 30.1°C), collected in Moorea 2011.</p><p><strong>Related Reference:</strong><br />Darren Brown, Peter J. Edmunds. Differences in the responses of three scleractinians and the hydrocoral Millepora platyphylla to ocean acidification. Marine Biology, 2016 (in press).</p><p><strong>Related Dataset:</strong><br /><a href="http://www.bco-dmo.org/dataset/641759" target="_blank">MarBio. 2016: tank conditions</a></p> Acquisition Description: <p>Calcifying cnidarians were collected from the back reef (~ 4 m depth) on the north shore of Moorea, French Polynesia, during January and April 2011. Fragments of Acropora pulchra, Pocillopora meandrina, massive Porites spp. (15% P. lobata and 85% P. lutea [Edmunds 2009]), and Millepora platyphylla were used to evaluate the effect of pCO2 and temperature on calcification. Massive Porites spp. and M. platyphylla were sampled using a pneumatic drill (McMaster-Carr, part #27755A17) fitted with a 4.1 cm diamond tip hole saw (McMaster-Carr, part #6930A43). The hole saw was used to remove cores ~ 4 cm diameter and ~ 3.8 cm long from adult colonies, and the holes were filled with non-toxic modeling clay (Van Aken Part #10117). To increase the likelihood that cores were genetically distinct, one core was taken from each colony, with sampled colonies distributed over 3 km of reef.</p><p>Freshly collected cores were placed in bags filled with seawater and transported to the Richard B. Gump South Pacific Research Station where they were immersed in tanks supplied with a constant flow of seawater from Cook’s Bay. Cores were prepared by removing excess skeleton extending &gt; 1.5 cm below the living tissue, and attaching the cores to numbered polyvinyl chloride (PVC) pipes (4.4 cm diameter and 2.0 cm long) with epoxy (Z Spar, #A788). To eliminate the possibility of fouling organisms accessing freshly cut skeleton, bare skeleton was covered in epoxy. A plastic screw was epoxied to the bottom of each core that was later used to attach them upright in racks placed in the tanks used for incubations. Following preparation, cores were returned to ~ 4 m depth in the back reef, where they were left to recover for 6 weeks. Recovery was evaluated from the presence of healthy c 124 oral tissue covering the formerly damaged edge of the skeleton.</p><p>Single branches of A. pulchra and P. meandrina were cut from colonies using bone shears, with each colony sampled once. Sampled colonies were ~ 10 m apart to increase the likelihood that they were genetically distinct. Branches were transported to the Richard B. Gump South Pacific Research Station where they were immersed in flowing seawater. Similar to the methods used for coral cores, branches of A. pulchra and P. meandrina were attached using epoxy to pieces of PVC pipe to make nubbins (Birkeland 1976). Care was taken to cover freshly fractured skeleton with epoxy, and to avoid damaging coral tissue during preparation. A plastic screw was attached to the base of the nubbins and used to hold them upright in plastic racks. Prior to beginning the treatments, coral cores and nubbins were placed in 150 L tanks under ambient conditions of 28.0°C, 370 micro-atm pCO2 and where illuminated with 400 W metal halide lamps (True 10,000K Hamilton Technology, Gardena, CA to an irradiance of ~ 600 micro-mol quanta m2 s-1 (measured with a 4p LI-193 quantum sensor and a LiCor LI-1400 meter) for 5 d to recover from the preparation procedure. The sampling method limited tissue damage to A. pulchra and P. meandrina, and therefore a shorter acclimation period was needed in comparison to massive Porites spp. and M. platyphylla.</p><p><strong>Experimental conditions and maintenance</strong></p><p>Treatments were created in 8 tanks (Aqua Logic, San Diego), each holding 150 L of seawater and regulated independently for temperature, light, and pCO2. Tanks were operated as closed146 circuit systems with filtered seawater (50 micro-m) from Cook’s Bay, with circulation provided by a pump (Rio 8HF, 2,082 L h-1). Light was supplied 147 by 400 W metal halide lamps (True 10,000K Hamilton Technology, Gardena, CA) at ~ 560 micro-mol quanta m-2s-1 (measured with a 4p LI-193 quantum sensor and a LiCor LI-1400 meter) in the range of photosynthetically active radiation (PAR, 400-700 nm). Lights were operated on a 12hr light-12hr dark photoperiod, beginning at 06:00 hrs and ending at 18:00 hrs. Temperatures were maintained at 28.0°C, which corresponded to the ambient seawater temperature in the back reef when the study was conducted, and 30.1°C which is close to the maximum temperature in this habitat (Putnam and Edmunds 2011). pCO2 treatments contrasted ambient conditions (~ 408 micro-atm) and 913 micro-atm pCO2, with the elevated value expected to occur within 100 y under the "stabilization without overshoot" representative concentration pathway (RCP 6.0) (van Vuuren et al. 2011). pCO2 treatments were created by bubbling ambient air or a mixture of ambient air and pure CO2 that was blended continually and monitored using an infrared gas analyzer (IRGA model S151, Qubit Systems). A solenoid-controlled, gas regulation system (Model A352, Qubit Systems, Ontario, Canada) regulated the flow of CO2 and air, with pCO2 logged on a PC running LabPro software (Vemier Software and Technology). Ambient air and the elevated pCO2 mixture were supplied at ~ 10-15 L min-1 to treatment tanks using pumps (Gast pump DOA-P704-AA, see Edmunds 2011).</p><p>The temperatures and pCO2 levels created four treatments with two tanks treatment-1: ambient temperature-ambient pCO2 (AT-ACO2), ambient temperature-high pCO2 (AT-HCO2), high temperature-ambient pCO2 (HT-ACO2) and high temperature-high pCO2 (HT-HCO2). Treatment conditions were monitored daily, with temperature measured at 08:00, 12:00 and 18:00 hrs using a digital thermometer (Fisher Scientific model #150778, ± 0.05 °C), and light intensities at 12:00 hrs using a Li-Cor LI-193 sensor attached t 170 o a LI-1400 meter. Seawater within each tank was replaced at 200 ml/min with filtered seawater (50 micro-m) pumped from Cook’s Bay.</p>
Funding provided by NSF Ocean Sciences (NSF OCE) Award Number: OCE-0417412 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0417412
Funding provided by NSF Ocean Sciences (NSF OCE) Award Number: OCE-1041270 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1041270
Funding provided by NSF Ocean Sciences (NSF OCE) Award Number: OCE-1026851 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1026851
completed
Dr Peter J Edmunds
California State University Northridge
818-677-2502
Department of Biology 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
peter.edmunds@csun.edu
pointOfContact
asNeeded
site
latitude
longitude
species
tank
temperature
pCO2
treatment
calcification
biomass
MCR_Edmunds
theme
None, User defined
LI-COR LI-193 PAR Sensor
In-situ incubator
Water Temperature Sensor
Automatic titrator
Light Meter
Conductivity Meter
ultrasonic cell disrupter
instrument
None, User defined
Moorea, French Polynesia
place
None, User defined
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.Solicitations issued under this program:NSF 10-530, FY 2010-FY2011NSF 12-500, FY 2012NSF 12-600, FY 2013NSF 13-586, FY 2014NSF 13-586 was the final solicitation that will be released for this program.PI Meetings:1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)NSF media releases for the Ocean Acidification Program:Press Release 10-186 NSF Awards Grants to Study Effects of Ocean AcidificationDiscovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)Press Release 13-102 World Oceans Month Brings Mixed News for OystersPress Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grantsPress Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
The effects of ocean acidification on the organismic biology and community ecology of corals, calcified algae, and coral reefs
http://www.bco-dmo.org/project/2242
The effects of ocean acidification on the organismic biology and community ecology of corals, calcified algae, and coral reefs
While coral reefs have undergone unprecedented changes in community structure in the past 50 y, they now may be exposed to their gravest threat since the Triassic. This threat is increasing atmospheric CO2, which equilibrates with seawater and causes ocean acidification (OA). In the marine environment, the resulting decline in carbonate saturation state (Omega) makes it energetically less feasible for calcifying taxa to mineralize; this is a major concern for coral reefs. It is possible that the scleractinian architects of reefs will cease to exist as a mineralized taxon within a century, and that calcifying algae will be severely impaired. While there is a rush to understand these effects and make recommendations leading to their mitigation, these efforts are influenced strongly by the notion that the impacts of pCO2 (which causes Omega to change) on calcifying taxa, and the mechanisms that drive them, are well-known. The investigators believe that many of the key processes of mineralization on reefs that are potentially affected by OA are only poorly known and that current knowledge is inadequate to support the scaling of OA effects to the community level. It is vital to measure organismal-scale calcification of key taxa, elucidate the mechanistic bases of these responses, evaluate community scale calcification, and finally, to conduct focused experiments to describe the functional relationships between these scales of mineralization.This project is a 4-y effort focused on the effects of Ocean Acidification (OA) on coral reefs at multiple spatial and functional scales. The project focuses on the corals, calcified algae, and coral reefs of Moorea, French Polynesia, establishes baseline community-wide calcification data for the detection of OA effects on a decadal-scale, and builds on the research context and climate change focus of the Moorea Coral Reef LTER.This project is a hypothesis-driven approach to compare the effects of OA on reef taxa and coral reefs in Moorea. The PIs will utilize microcosms to address the impacts and mechanisms of OA on biological processes, as well as the ecological processes shaping community structure. Additionally, studies of reef-wide metabolism will be used to evaluate the impacts of OA on intact reef ecosystems, to provide a context within which the experimental investigations can be scaled to the real world, and critically, to provide a much needed reference against which future changes can be gauged.The following publications and data resulted from this project:2016 Edmunds P.J. and 15 others. Integrating the effects of ocean acidification across functional scales on tropical coral reefs. Bioscience (in press Feb 2016) **not yet available**2016 Comeau S, Carpenter RC, Lantz CA, Edmunds PJ. Parameterization of the response of calcification to temperature and pCO2 in the coral Acropora pulchra and the alga Lithophyllum kotschyanum. Coral Reefs (in press Feb 2016)2016 Brown D., Edmunds P.J. Differences in the responses of three scleractinians and the hydrocoral Millepora platyphylla to ocean acidification. Marine Biology (in press Feb 2016) **available soon**MarBio. 2016: calcification and biomassMarBio. 2016: tank conditions2016 Comeau, S., Carpenter, R.C., Edmunds, P.J. Effects of pCO2 on photosynthesis and respiration of tropical scleractinian corals and calcified algae. ICES Journal of Marine Science doi:10.1093/icesjms/fsv2672015 Evensen NR, Edmunds PJ, Sakai K. Effects of pCO2 on the capacity for spatial competition by the corals Montipora aequituberculata and massive Porites spp. Marine Ecology Progress Series 541: 123–134. doi: 10.3354/meps11512MEPS 2015: chemistryMEPS 2015: field surveyMEPS 2015: linear extensionDownload data for this publication (Excel file)2015 Comeau S., Lantz C. A., Edmunds P. J., Carpenter R. C. Framework of barrier reefs threatened by ocean acidification. Global Change Biology doi: 10.1111/gcb.130232015 Comeau, S., Carpenter, R. C., Lantz, C. A., and Edmunds, P. J. Ocean acidification accelerates dissolution of experimental coral reef communities, Biogeosciences, 12, 365-372, doi:10.5194/bg-12-365-2015.calcification rates - flume exptcarbonate chemistry - flume exptExternal data repository: http://doi.pangaea.de/10.1594/PANGAEA.8479862014 Comeau S, Carpenter RC, Edmunds PJ. Effects of irradiance on the response of the coral Acropora pulchra and the calcifying alga Hydrolithon reinboldii to temperature elevation and ocean acidification. Journal of Experimental Marine Biology and Ecology (in press)2014 Comeau S, Carpenter RC, Nojiri Y, Putnam HM, Sakai K, Edmunds PJ. Pacific-wide contrast highlights resistance of reef calcifiers to ocean acidification. Royal Society of London (B) 281: doi.org/10.1098/rspb.2014.1339External data repository: http://doi.pangaea.de/10.1594/PANGAEA.8328342014 Comeau, S., Edmunds, P. J., Lantz, C. A., & Carpenter, R. C. Water flow modulates the response of coral reef communities to ocean acidification. Scientific Reports, 4. doi:10.1038/srep06681calcification rates - flume exptcarbonate chemistry - flume expt2014 Comeau, S., Edmunds, P. J., Spindel, N. B., & Carpenter, R. C. Fast coral reef calcifiers are more sensitive to ocean acidification in short-term laboratory incubations. Limnology and Oceanography, 59(3), 1081–1091. doi:10.4319/lo.2014.59.3.1081algae_calcificationcoral_calcificationExternal data repository: http://doi.pangaea.de/10.1594/PANGAEA.8325842014 Comeau S, Edmunds PJ, Spindel NB, Carpenter RC. Diel pCO2 oscillations modulate the response of the coral Acropora hyacinthus to ocean acidification. Marine Ecology Progress Series 453: 28-352013 Comeau, S, Carpenter, RC, Edmunds PJ. Response to coral reef calcification: carbonate, bicarbonate and proton flux under conditions of increasing ocean acidification. Proceedings of the Royal Society of London 280: doi.org/10.1098/rspb.2013.11532013 Comeau S, Carpenter RC. Edmunds PJ. Effects of feeding and light intensity on the response of the coral Porites rus to ocean acidification. Marine Biology 160: 1127-1134External data repository: http://doi.pangaea.de/10.1594/PANGAEA.8298152013 Comeau, S., Edmunds, P. J., Spindel, N. B., Carpenter, R. C. The responses of eight coral reef calcifiers to increasing partial pressure of CO2 do not exhibit a tipping point. Limnol. Oceanogr. 58, 388–398.algae_calcificationcoral_calcificationExternal data repository: http://doi.pangaea.de/10.1594/PANGAEA.8336872012 Comeau, S., Carpenter, R. C., & Edmunds, P. J. Coral reef calcifiers buffer their response to ocean acidification using both bicarbonate and carbonate. Proceedings of the Royal Society B: Biological Sciences, 280(1753), 20122374. doi:10.1098/rspb.2012.2374carbonate_chemistrylight_dark_calcificationmean_calcificationExternal data repository: http://doi.pangaea.de/10.1594/PANGAEA.832834
OA_Corals
largerWorkCitation
project
eng; USA
biota
oceans
Moorea, French Polynesia
-149.826
-149.826
-17.4907
-17.4907
2011-01-01
2011-04-30
0
BCO-DMO catalogue of parameters from Calcification Rates and Biomass of 4 Coral Species, 2 Temperatures and 2 pCO2 Levels from Experiments at LTER site in Moorea, French Polynesia, 2011 (OA_Corals project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/641748.rdf
Name: location
Units: unitless
Description: location of experiment
http://lod.bco-dmo.org/id/dataset-parameter/641749.rdf
Name: lat
Units: decimal degrees
Description: latitude; north is positive
http://lod.bco-dmo.org/id/dataset-parameter/641750.rdf
Name: lon
Units: decimal degrees
Description: longitude; east is positive
http://lod.bco-dmo.org/id/dataset-parameter/641751.rdf
Name: species
Units: unitless
Description: species used in the study: Ap (Acropora pulchra); Mipl (Millepora platyphylla); MP (massive Porites spp.) ; Pm (Pocillopora meandrina)
http://lod.bco-dmo.org/id/dataset-parameter/641752.rdf
Name: tank
Units: unitless
Description: tank number
http://lod.bco-dmo.org/id/dataset-parameter/641753.rdf
Name: temp
Units: unitless
Description: tank temperature: AT=ambient (28.0 C); HT=high (30.1 C)
http://lod.bco-dmo.org/id/dataset-parameter/641754.rdf
Name: pCO2
Units: unitless
Description: tank CO2 concentration levels: ACO2 for ambient (408 micro-atm) and HCO2 for high (913 micro-atm)
http://lod.bco-dmo.org/id/dataset-parameter/641755.rdf
Name: treatment
Units: unitless
Description: AT-ACO2 = ambient temperature; ambient CO2; AT-HCO2 = ambient temperature-high CO2; HT-ACO2 = high temperature-ambient CO2; HT-HCO2 = high temperature-high CO2
http://lod.bco-dmo.org/id/dataset-parameter/641756.rdf
Name: calcification
Units: cm-2 day-1
Description: calcification rate: ACO2 for ambient (408 µatm) and HCO2 for high (913 µatm) CO2 concentration levels
http://lod.bco-dmo.org/id/dataset-parameter/641757.rdf
Name: biomass
Units: mg mg-1
Description: coral biomass
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
http://vocab.nerc.ac.uk/collection/P09/current/LATX/
Name: LATITUDE
Description: The angular distance north or south from the equator, measured along the meridian of the point of interest.
http://vocab.nerc.ac.uk/collection/P09/current/LONX/
Name: LONGITUDE
Description: The angular distance east or west east or west of Greenwich to the point of interest.
http://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
Name: Temperature (ITS-90) of the water body
Description: The degree of hotness at any position within a volume of fresh or salt water expressed against the International Temperature Scale 1990 (ITS-90) calibration standard.
http://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/
Name: Partial pressure of carbon dioxide {pCO2} in the water body by computation from pH and alkalinity
Description: Unavailable
Parameters for this dataset
physicalMeasurement
location
site
PI-supplied Description: location of experiment; General Description: Sampling site identification.
http://lod.bco-dmo.org/id/dataset-parameter/641748.rdf
unitless
lat
latitude
PI-supplied Description: latitude; north is positive; General Description: latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes NERC Description: The angular distance north or south from the equator, measured along the meridian of the point of interest.
http://lod.bco-dmo.org/id/dataset-parameter/641749.rdf
decimal degrees
lon
longitude
PI-supplied Description: longitude; east is positive; General Description: longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datsets as degrees, minutes NERC Description: The angular distance east or west east or west of Greenwich to the point of interest.
http://lod.bco-dmo.org/id/dataset-parameter/641750.rdf
decimal degrees
species
species
PI-supplied Description: species used in the study: Ap (Acropora pulchra); Mipl (Millepora platyphylla); MP (massive Porites spp.) ; Pm (Pocillopora meandrina); General Description: a taxonomic binomial that consists of a genus name followed by the species name of an organism
http://lod.bco-dmo.org/id/dataset-parameter/641751.rdf
unitless
tank
tank
PI-supplied Description: tank number; General Description: Tank Number or Tank Id. Used to identify tanks/containers used in various experiments
http://lod.bco-dmo.org/id/dataset-parameter/641752.rdf
unitless
temp
water temperature
PI-supplied Description: tank temperature: AT=ambient (28.0 C); HT=high (30.1 C); General Description: water temperature at measurement depth NERC Description: The degree of hotness at any position within a volume of fresh or salt water expressed against the International Temperature Scale 1990 (ITS-90) calibration standard.
http://lod.bco-dmo.org/id/dataset-parameter/641753.rdf
unitless
pCO2
pCO2
PI-supplied Description: tank CO2 concentration levels: ACO2 for ambient (408 micro-atm) and HCO2 for high (913 micro-atm); General Description: Partial pressure of carbon dioxide {pCO2} in the water body by computation from pH and alkalinity. NERC Description: Unavailable
http://lod.bco-dmo.org/id/dataset-parameter/641754.rdf
unitless
treatment
treatment
PI-supplied Description: AT-ACO2 = ambient temperature; ambient CO2; AT-HCO2 = ambient temperature-high CO2; HT-ACO2 = high temperature-ambient CO2; HT-HCO2 = high temperature-high CO2; General Description: Experimental conditions applied to experimental units. In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.
http://lod.bco-dmo.org/id/dataset-parameter/641755.rdf
unitless
calcification
no standard parameter
PI-supplied Description: calcification rate: ACO2 for ambient (408 µatm) and HCO2 for high (913 µatm) CO2 concentration levels; General Description: association with a community-wide standard parameter is not yet defined
http://lod.bco-dmo.org/id/dataset-parameter/641756.rdf
cm-2 day-1
biomass
biomass
PI-supplied Description: coral biomass; General Description: wet weight of a specific taxa/group identified, in units milligrams per meter^3
http://lod.bco-dmo.org/id/dataset-parameter/641757.rdf
mg mg-1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://dx.doi.org/10.1575/1912/bco-dmo.641945
download
dataset
<p>Calcifying cnidarians were collected from the back reef (~ 4 m depth) on the north shore of Moorea, French Polynesia, during January and April 2011. Fragments of Acropora pulchra, Pocillopora meandrina, massive Porites spp. (15% P. lobata and 85% P. lutea [Edmunds 2009]), and Millepora platyphylla were used to evaluate the effect of pCO2 and temperature on calcification. Massive Porites spp. and M. platyphylla were sampled using a pneumatic drill (McMaster-Carr, part #27755A17) fitted with a 4.1 cm diamond tip hole saw (McMaster-Carr, part #6930A43). The hole saw was used to remove cores ~ 4 cm diameter and ~ 3.8 cm long from adult colonies, and the holes were filled with non-toxic modeling clay (Van Aken Part #10117). To increase the likelihood that cores were genetically distinct, one core was taken from each colony, with sampled colonies distributed over 3 km of reef.</p><p>Freshly collected cores were placed in bags filled with seawater and transported to the Richard B. Gump South Pacific Research Station where they were immersed in tanks supplied with a constant flow of seawater from Cook’s Bay. Cores were prepared by removing excess skeleton extending &gt; 1.5 cm below the living tissue, and attaching the cores to numbered polyvinyl chloride (PVC) pipes (4.4 cm diameter and 2.0 cm long) with epoxy (Z Spar, #A788). To eliminate the possibility of fouling organisms accessing freshly cut skeleton, bare skeleton was covered in epoxy. A plastic screw was epoxied to the bottom of each core that was later used to attach them upright in racks placed in the tanks used for incubations. Following preparation, cores were returned to ~ 4 m depth in the back reef, where they were left to recover for 6 weeks. Recovery was evaluated from the presence of healthy c 124 oral tissue covering the formerly damaged edge of the skeleton.</p><p>Single branches of A. pulchra and P. meandrina were cut from colonies using bone shears, with each colony sampled once. Sampled colonies were ~ 10 m apart to increase the likelihood that they were genetically distinct. Branches were transported to the Richard B. Gump South Pacific Research Station where they were immersed in flowing seawater. Similar to the methods used for coral cores, branches of A. pulchra and P. meandrina were attached using epoxy to pieces of PVC pipe to make nubbins (Birkeland 1976). Care was taken to cover freshly fractured skeleton with epoxy, and to avoid damaging coral tissue during preparation. A plastic screw was attached to the base of the nubbins and used to hold them upright in plastic racks. Prior to beginning the treatments, coral cores and nubbins were placed in 150 L tanks under ambient conditions of 28.0°C, 370 micro-atm pCO2 and where illuminated with 400 W metal halide lamps (True 10,000K Hamilton Technology, Gardena, CA to an irradiance of ~ 600 micro-mol quanta m2 s-1 (measured with a 4p LI-193 quantum sensor and a LiCor LI-1400 meter) for 5 d to recover from the preparation procedure. The sampling method limited tissue damage to A. pulchra and P. meandrina, and therefore a shorter acclimation period was needed in comparison to massive Porites spp. and M. platyphylla.</p><p><strong>Experimental conditions and maintenance</strong></p><p>Treatments were created in 8 tanks (Aqua Logic, San Diego), each holding 150 L of seawater and regulated independently for temperature, light, and pCO2. Tanks were operated as closed146 circuit systems with filtered seawater (50 micro-m) from Cook’s Bay, with circulation provided by a pump (Rio 8HF, 2,082 L h-1). Light was supplied 147 by 400 W metal halide lamps (True 10,000K Hamilton Technology, Gardena, CA) at ~ 560 micro-mol quanta m-2s-1 (measured with a 4p LI-193 quantum sensor and a LiCor LI-1400 meter) in the range of photosynthetically active radiation (PAR, 400-700 nm). Lights were operated on a 12hr light-12hr dark photoperiod, beginning at 06:00 hrs and ending at 18:00 hrs. Temperatures were maintained at 28.0°C, which corresponded to the ambient seawater temperature in the back reef when the study was conducted, and 30.1°C which is close to the maximum temperature in this habitat (Putnam and Edmunds 2011). pCO2 treatments contrasted ambient conditions (~ 408 micro-atm) and 913 micro-atm pCO2, with the elevated value expected to occur within 100 y under the "stabilization without overshoot" representative concentration pathway (RCP 6.0) (van Vuuren et al. 2011). pCO2 treatments were created by bubbling ambient air or a mixture of ambient air and pure CO2 that was blended continually and monitored using an infrared gas analyzer (IRGA model S151, Qubit Systems). A solenoid-controlled, gas regulation system (Model A352, Qubit Systems, Ontario, Canada) regulated the flow of CO2 and air, with pCO2 logged on a PC running LabPro software (Vemier Software and Technology). Ambient air and the elevated pCO2 mixture were supplied at ~ 10-15 L min-1 to treatment tanks using pumps (Gast pump DOA-P704-AA, see Edmunds 2011).</p><p>The temperatures and pCO2 levels created four treatments with two tanks treatment-1: ambient temperature-ambient pCO2 (AT-ACO2), ambient temperature-high pCO2 (AT-HCO2), high temperature-ambient pCO2 (HT-ACO2) and high temperature-high pCO2 (HT-HCO2). Treatment conditions were monitored daily, with temperature measured at 08:00, 12:00 and 18:00 hrs using a digital thermometer (Fisher Scientific model #150778, ± 0.05 °C), and light intensities at 12:00 hrs using a Li-Cor LI-193 sensor attached t 170 o a LI-1400 meter. Seawater within each tank was replaced at 200 ml/min with filtered seawater (50 micro-m) pumped from Cook’s Bay.</p>
<p><strong>BCO-DMO Processing:</strong></p><p>- added conventional header with dataset name, PI name, version date, reference information<br />- renamed parameters to BCO-DMO standard<br />- added location, lat and lon columns</p>
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PI Supplied Instrument Name: PI Supplied Instrument Description:4p LI-193 quantum sensor Instrument Name: LI-COR LI-193 PAR Sensor Instrument Short Name:LI-COR LI-193 PAR Instrument Description: The LI-193 Underwater Spherical Quantum Sensor uses a Silicon Photodiode and glass filters encased in a waterproof housing to measure PAR (in the 400 to 700 nm waveband) in aquatic environments. Typical output is in micromol s-1 m-2. The LI-193 Sensor gives an added dimension to underwater PAR measurements as it measures photon flux from all directions. This measurement is referred to as Photosynthetic Photon Flux Fluence Rate (PPFFR) or Quantum Scalar Irradiance. This is important, for example, when studying phytoplankton, which utilize radiation from all directions for photosynthesis. LI-COR began producing Spherical Quantum Sensors in 1979; serial numbers for the LI-193 begin with SPQA-XXXXX (licor.com). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0458/
PI Supplied Instrument Name: PI Supplied Instrument Description:150 L tanks Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on shipboard or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/
PI Supplied Instrument Name: Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/
PI Supplied Instrument Name: PI Supplied Instrument Description:Open cell potentiometric titrator (Model T50, Mettler-Toledo, Columbus, OH) fitted with a DG115-SC pH probe (Mettler-Toledo, Columbus, OH) Instrument Name: Automatic titrator Instrument Short Name:Automatic titrator Instrument Description: Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
PI Supplied Instrument Name: PI Supplied Instrument Description:LiCor LI-1400 meter Instrument Name: Light Meter Instrument Short Name:Light Meter Instrument Description: Light meters are instruments that measure light intensity. Common units of measure for light intensity are umol/m2/s or uE/m2/s (micromoles per meter squared per second or microEinsteins per meter squared per second). (example: LI-COR 250A)
PI Supplied Instrument Name: PI Supplied Instrument Description:YSI 3100 conductivity meter Instrument Name: Conductivity Meter Instrument Short Name:Conductivity Meter Instrument Description: Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.
PI Supplied Instrument Name: PI Supplied Instrument Description:Ultrasonic dismembrator (Fisher model 216 15-338-550; fitted with a 3.2 mm diameter probe, Fisher 15-338-67) Instrument Name: ultrasonic cell disrupter Instrument Short Name:sonicator Instrument Description: Instrument that applies sound energy to agitate particles in a sample.
Deployment: MCR_Edmunds
MCR_Edmunds
Richard B Gump Research Station - Moorea LTER
Richard B Gump Research Station - Moorea LTER
island
MCR_Edmunds
Dr Peter J Edmunds
California State University Northridge