Auxiliary material for Paper 2009GL037643 High-sensitivity measurement of diverse vascular plant-derived biomarkers in high-altitude ice cores Matthew C. Makou Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, USA Byrd Polar Research Center, Ohio State University, Columbus, Ohio, USA Lonnie G. Thompson Byrd Polar Research Center, Ohio State University, Columbus, Ohio, USA Daniel B. Montlucon and Timothy I. Eglinton Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, USA Makou, M. C., L. G. Thompson, D. B. Montlucon, and T. I. Eglinton (2009), High-sensitivity measurement of diverse vascular plant-derived biomarkers in high-altitude ice cores, Geophys. Res. Lett., 36, L13501, doi:10.1029/2009GL037643. Introduction The auxiliary material files associated with this work contain an expanded methods section, ice core location information, and details pertaining to the identification and quantification of biomarkers discussed in the paper. An expanded methods section (Text S1) describes sample handling and preparation, analytical conditions, and data treatment. Ice core sampling sites are shown in Figure S1 and their geographical coordinates are given in Table S1. Selected mass spectra used in compound identification are shown in Figure S2, and information pertaining to biomarker quantification is listed in a data summary table (Table S2). 1. 2009gl037643-txts01.txt Additional information about sampling and the analytical methods used in this study. 2. 2009gl037643-fs01.eps Locations of sample sites. 3. 2009gl037643-fs02.eps Mass spectra of key biomarkers detected in the ice core samples, produced through TOF-MS analysis. Results for the following compounds are presented, along with molecular diagrams: a) dehydroabietane, b) dehydroabietic aldehyde, c) sugiol, d) tremetone, e) the C22:1 alkyl amide (docosenamide), and f) the C22:1 alkyl nitrile (docosenenitrile). Molecular ions are depicted (M+) for each compound and diagnostic masses are labeled with their m/z value. These analytical spectra may contain background ionic contributions. 4. 2009gl037643-ts01.txt Ice core geographical information. 4.1 Column "Ice Core", sample site. 4.2 Column "Latitude", latitude of ice field. 4.3 Column "Longitude", longitude of ice field. 4.4 Column "Elevation", elevation of ice field in meters above sea level. 5. 2009gl037643-ts02.txt Information pertaining to the analysis of each compound. The reported quantitative ions were summed to produce extracted ion chromatograms. The ice cores in which each compound was detected (S=Sajama, H=Huascaran, P=Puruogangri, D=Dasuopu, K=Kilimanjaro) are listed in the Geographic Distribution column. At least three separate ice aliquots were analyzed for every modern sample, and both mean concentration and standard deviation values were determined for each set of measurements; the ranges of these values across all five sites are reported for each compound in the Concentration Range and Reproducibility columns, respectively. The highest biomarker concentration observed in either blank is also reported. 5.1 Column "Compound", name of analyte. 5.2 Column "Quantitative Ions (m/z)", ions used to generate the extracted ion chromatograms for each compound and achieve quantification. 5.3 Column "Geographic Distribution", the ice cores in which each compound was detected (S=Sajama, H=Huascaran, P=Puruogangri, D=Dasuopu, K=Kilimanjaro). 5.4 Column "Concentration Range (pg/ml)", the range of concentrations (mean of replicate measurements) in pg/ml observed for each compound across all sample locations and time horizons. 5.5 Column "Highest Blank Level (pg/ml)", the highest abundance of each compound in pg/ml observed in either of the blanks. 5.6 Column "Reproducibility (±pg/ml)", the range of standard deviation values determined for each compound (from replicate measurements) across all sample locations and time horizons.