http://lod.bco-dmo.org/id/dataset/866648
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-12-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Quantum yield of photosystem II (Fv/Fm) from an experiment examining the effect of Sr on the coccolithophore Scyphosphaera apsteinii calcification
2021-12-10
publication
2021-12-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-01-10
publication
https://doi.org/10.26008/1912/bco-dmo.866648.1
Alison Taylor
University of North Carolina - Wilmington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
documentDigital
Cite this dataset as: Taylor, A. (2021) Quantum yield of photosystem II (Fv/Fm) from an experiment examining the effect of Sr on the coccolithophore Scyphosphaera apsteinii calcification. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-12-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.866648.1 [access date]
Sr Experiment: Fv/Fm Dataset Description: Acquisition Description: <p><strong>Experiment Overview:</strong><br />
The effect of strontium (Sr) on the coccolithophore <em>Scyphosphaera apsteinii</em> calcification was assessed over a 10 d period. This species of coccolithophore has unusually high levels of Sr in its calcite coccoliths as detected with Energy-dispersive X-ray spectroscopy (EDS), whereas Si levels are undetectable with EDS. The goal of the experiment was to determine whether Sr in seawater plays a significant role in coccolith production and/or coccolith crystal morphology. <em>S. apsteinii </em>was acclimated and grown in a range of Sr concentrations (deplete: 0.33 mmol/mol Sr/Ca, ambient: 9 mmol/mol Sr/Ca, and higher than ambient: 36 and 72 mmol/mol Sr/Ca). All treatments had four replicate flasks. Aliquots of cultures for cell counts and Fv/Fm were taken every two days between the start (T0 for cell counts, T4 for Fv/Fm) and end (T10) of the experiment. When cells were at mid-exponential phase (T4-T6) aliquots were collected for scanning electron microscopy (SEM) and EDS analysis to observe morphology and determine Sr incorporation into calcite coccoliths, respectively.</p>
<p>The laboratory experiments were conducted in March 2019 at&nbsp;the University of North Carolina - Wilmington. SEM/EDS analysis was done at the Analytical Instrumentation Facility at NC State from April 19th to May 9th, 2019.</p>
<p><strong>Fv/Fm:</strong><br />
The quantum yield of photosystem II (Fv/Fm) was determined using a fluorometer by pipetting approximately 1.5 mL of sample from each flask into a cuvette. The cuvette was dark adapted at room temperature for 15 minutes. Three measurements for steady state fluorescence (Ft) and Fv/FM per cuvette were taken after gentle inversion to resuspend cells.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1638838 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1638838
completed
Alison Taylor
University of North Carolina - Wilmington
910-962-2176
Department of Biology and Marine Biology 601 South College Road
Wilmington
NC
28403
USA
taylora@uncw.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sr_Concentration
Flask_Replicate
Sampling_Day
Ft
FvFm
AquaPen AP 100 fluorometer (PSI, Drasov, Czech Republic)
theme
None, User defined
treatment
replicate
time_elapsed
fluorescence
Fv to Fm ratio
featureType
BCO-DMO Standard Parameters
Fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
NSFGEO-NERC: An unexpected requirement for silicon in coccolithophore calcification: physiological, ecological and evolutionary implications
https://www.bco-dmo.org/project/737936
NSFGEO-NERC: An unexpected requirement for silicon in coccolithophore calcification: physiological, ecological and evolutionary implications
<p><em>NSF Abstract:</em><br />
Biomineralization by marine phytoplankton has had a profound impact on our planet. The production of special cell wall material, calcite coccoliths by coccolithophores and silica frustules by diatoms, are major drivers in global biogeochemical cycles, but the underlying cellular processes remain poorly understood. It is widely considered that calcification in coccolithophores occurs through a very different process to silicification in diatoms, however some ecologically important coccolithophore lineages possess diatom-like silicon (Si) transport systems and have an absolute requirement for Si during coccolith formation. Importantly, the abundant bloom-forming coccolithophores such as Emiliania huxleyi exhibit no requirement for Si. There is a clear need to understand how these different physiological requirements for dissolved Si have driven the ecology and evolution of the coccolithophores. The project will yield a more complete understanding of the Si requirements of coccolithophores, its role in the calcification process, and the impacts of Si availability on the biogeography of these important bloom forming phytoplankton. The results are expected to strengthen our ability to predict the responses of coccolithophores to short and long-term environmental change, and therefore the consequences for the marine biogeochemical cycles in which they participate. In addition to the scientific outcomes, the project provides independent research opportunities to a diverse pool of undergraduate students, provide interdisciplinary training for graduate students, and facilitate the professional development of post-doctoral researchers. Public engagement in the research is facilitated through participant involvement in regional science festivals, public outreach events, production of educational resources, and targeted K-12 summer camp activities.</p>
<p>Calcification in coccolithophores appears to represent a distinct process from silicification in diatoms, another major group of biomineralized phytoplankton. The apparent absence of a requirement for silicon (Si) in coccolithophores has been proposed to play a critical role in their ability to out-compete the otherwise dominant diatoms in areas of low dissolved Si availability. However, the investigators recently demonstrated that some globally important coccolithophores possess diatom-like Si transporters and exhibit an obligate requirement for Si in the calcification process. This discovery has important implications both for phytoplankton ecology and for the evolution of biomineralization. Using a range of physiological, molecular and computational approaches the project will 1) Establish Si requirements of ecologically important coccolithophore groups; 2) Determine the physiological role of Si in coccolithophores; 3) Determine the evolutionary events leading to the differing requirements for Si in calcification; 4) Examine the ecological distribution of Si-requiring coccolithophores, and 5) Determine the impact of the Si requirement on coccolithophore ecology. This project therefore integrates the molecular identification of genes (Si transporters), the physiological role of these transporters, and ecosystem scale models in order to examine how the requirement for Si influences ecosystem functioning and coccolithophore biogeography. The results of this work provides essential data that describes the cellular mechanisms of calcification and the range of physiological diversity between major coccolithophore lineages. The research also explores a previously unforeseen aspect of phytoplankton ecology; examining how the differing requirements for Si in calcifying coccolithophores may have shaped competitive interactions with other phytoplankton over both contemporary and evolutionary timescales. Overall, the research provides novel insights into physiology, ecology and evolution of coccolithophores, including information on how and why coccoliths are produced, which is currently poorly understood. This information is vital in order to understand how coccolithophores have been influenced by past changes in the Earth's climate, and their potential responses to future oceans.</p>
Coccolithophore Silicon Requirements
largerWorkCitation
project
eng; USA
oceans
2019-03-01
2019-03-31
0
BCO-DMO catalogue of parameters from Quantum yield of photosystem II (Fv/Fm) from an experiment examining the effect of Sr on the coccolithophore Scyphosphaera apsteinii calcification
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/866658.rdf
Name: Sr_Concentration
Units: micromoles per liter Si (umol/L)
Description: The Sr concentration of the growth media that S. apsteinii was exposed to. The concentrations were 3.3, 90, 360, and 720 micromole.
http://lod.bco-dmo.org/id/dataset-parameter/866659.rdf
Name: Flask_Replicate
Units: unitless
Description: The replicate flask number for each Sr treatment. Each Sr concentration had 4 replicate flasks.
http://lod.bco-dmo.org/id/dataset-parameter/866660.rdf
Name: Sampling_Day
Units: days (d)
Description: The day the aliquots were taken from flasks and cells were counted
http://lod.bco-dmo.org/id/dataset-parameter/866661.rdf
Name: Ft
Units: relative units
Description: Steady state fluorescence. Any reading below 200 indicated that cell density was too low to reliably measure Fv/Fm, and therefore Fv/Fm was not determined on those days.
http://lod.bco-dmo.org/id/dataset-parameter/866662.rdf
Name: FvFm
Units: relative units
Description: Quantum efficiency of photosystem II; a measurement of photosynthetic health.
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://www.bco-dmo.org/dataset/866648/data/download
download
onLine
dataset
<p><strong>Experiment Overview:</strong><br />
The effect of strontium (Sr) on the coccolithophore <em>Scyphosphaera apsteinii</em> calcification was assessed over a 10 d period. This species of coccolithophore has unusually high levels of Sr in its calcite coccoliths as detected with Energy-dispersive X-ray spectroscopy (EDS), whereas Si levels are undetectable with EDS. The goal of the experiment was to determine whether Sr in seawater plays a significant role in coccolith production and/or coccolith crystal morphology. <em>S. apsteinii </em>was acclimated and grown in a range of Sr concentrations (deplete: 0.33 mmol/mol Sr/Ca, ambient: 9 mmol/mol Sr/Ca, and higher than ambient: 36 and 72 mmol/mol Sr/Ca). All treatments had four replicate flasks. Aliquots of cultures for cell counts and Fv/Fm were taken every two days between the start (T0 for cell counts, T4 for Fv/Fm) and end (T10) of the experiment. When cells were at mid-exponential phase (T4-T6) aliquots were collected for scanning electron microscopy (SEM) and EDS analysis to observe morphology and determine Sr incorporation into calcite coccoliths, respectively.</p>
<p>The laboratory experiments were conducted in March 2019 at&nbsp;the University of North Carolina - Wilmington. SEM/EDS analysis was done at the Analytical Instrumentation Facility at NC State from April 19th to May 9th, 2019.</p>
<p><strong>Fv/Fm:</strong><br />
The quantum yield of photosystem II (Fv/Fm) was determined using a fluorometer by pipetting approximately 1.5 mL of sample from each flask into a cuvette. The cuvette was dark adapted at room temperature for 15 minutes. Three measurements for steady state fluorescence (Ft) and Fv/FM per cuvette were taken after gentle inversion to resuspend cells.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- removed units from data columns so values can be typed as numeric;<br />
- replaced "ND" with "nd".</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
AquaPen AP 100 fluorometer (PSI, Drasov, Czech Republic)
AquaPen AP 100 fluorometer (PSI, Drasov, Czech Republic)
PI Supplied Instrument Name: AquaPen AP 100 fluorometer (PSI, Drasov, Czech Republic) PI Supplied Instrument Description:Fv/Fm is a measure of photosynthetic efficiency by measuring the quantum efficiency of photosystem II; a tool for assessing cell health. An AquaPen AP 100 fluorometer (PSI, Drasov, Czech Republic) was used to assess Fv/Fm. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/