http://lod.bco-dmo.org/id/dataset/821539
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Primary production from the San Pedro Ocean Time-series (SPOT) measured between 2014 to 2016
2020-08-20
publication
2020-08-20
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-20
publication
https://doi.org/10.26008/1912/bco-dmo.821539.1
Alyson Santoro
University of California-Santa Barbara
principalInvestigator
Douglas G. Capone
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
documentDigital
Cite this dataset as: Santoro, A., Capone, D. G. (2020) Primary production from the San Pedro Ocean Time-series (SPOT) measured between 2014 to 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-20 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.821539.1 [access date]
Primary production measured using H13CO3 from the San Pedro Ocean Time-series (SPOT) measured between 2014 to 2016 Dataset Description: Acquisition Description: <p>From 2014 to 2015, water samples were collected using a 12 x 12 L Niskin bottle rosette equipped with a conductivity, temperature, and density (CTD) instrument package (SBE 9plus, Sea-Bird Electronics, Bellevue, Washington, USA), including dissolved oxygen (SBE 43) and photosynthetically available radiation (PAR, LI-COR, Biospherical Instruments Inc., San Diego, California, USA) sensors. Due to CTD failure, samples collected in 2015 and 2016 were collected primarily using manually triggered Go-Flo bottles and depths were chosen primarily using a profiling natural fluorometer (PNF) system as well as secchi disk.</p>
<p>Rates of primary production were measured by quantifying the rate of CO2 fixation via H¹³CO₃ uptake. Samples were analyzed using an IsoPrime continuous flow isotope ratio mass spectrometer. Quadruplicate samples were collected and amended to a final concentration of ~25 µmol L-1 of H¹³CO₃. Incubations were carried out for 24 hrs at ambient temperature and light.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1437310 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1437310
completed
Alyson Santoro
University of California-Santa Barbara
805-893-5318
2155 Marine Biotech Lab
Santa Barbara
CA
93106
USA
asantoro@ucsb.edu
pointOfContact
Douglas G. Capone
University of Southern California
213-740-2772
616 Trousdale Parkway AHF 246
Los Angeles
CA
90089
USA
capone@usc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
date
depth
pp_1
pp_2
pp_3
Go-Flo bottles
Niskin bottle
IsoPrime continuous flow isotope ratio mass spectrometer
profiling natural fluorometer
SBE 9plus
secchi disk
theme
None, User defined
date
depth
primary production
featureType
BCO-DMO Standard Parameters
GO-FLO Bottle
Niskin bottle
Isotope-ratio Mass Spectrometer
Fluorometer
CTD Sea-Bird 9
Secchi Disc
instrument
BCO-DMO Standard Instruments
N-SPOT_Yellowfin_Cruises
service
Deployment Activity
an Pedro Ocean Time-series (SPOT)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: New Approaches to New Production
https://dornsife.usc.edu/labs/capone
Collaborative Research: New Approaches to New Production
<p><em>NSF Award Abstract:</em><br />
Coastal marine ecosystems are seasonally dynamic and highly productive. Phytoplankton populations shift from nutrient replete conditions in the spring to nutrient poor conditions in other seasons. The San Pedro Ocean Time-series (SPOT), located 17 km offshore between Los Angeles Harbor and Catalina Island, is a representative and accessible model coastal system with regular sampling and a substantial archive of relevant observations. The SPOT program has cataloged the dynamics, diversity, and productivity of microbial populations since 2000. With rising carbon dioxide (CO2) concentrations and resulting decreases in surface pH, it is critically important to understand the nutrient controls on primary production in coastal waters and the capacity of coastal ecosystems to sequester CO2. This project will examine rates of primary production, nitrogen uptake associated with primary production, and the oxidation of ammonium to nitrate (nitrification), at SPOT over two seasonal cycles. It will also contribute to the development of human resources in the marine sciences through the training of undergraduate and graduate students at the University of Southern California and the University of Maryland. The researchers participate in education outreach activities (e.g. through the Centers for Ocean Sciences Education Excellence programs), and will incorporate findings from this study in those presentations.</p>
<p>This project will investigate primary production and nitrogen (N) dynamics at SPOT and specifically implement an analysis of new production. The new production conceptual model has been a powerful organizing principle in biological oceanography and provides a means to constrain the amount of primary production that may be exported or "sequestered" from the system. Despite qualifications to the definitions of new and regenerated forms of N as originally articulated, the concept has, for the most part, been narrowly applied, specifying nitrate as the primary form of new N, and ammonium as the predominant recycled form. Evidence continues to accumulate that these definitions may warrant expansion. N fixation can be at times a substantial source of new N; similarly, forms of dissolved organic N (e.g., urea) may contribute significantly to recycled production, but the specific organisms taking part in these transformations are still uncertain. Nitrification in the upper water column may also compromise the strict definitions of new and recycled N. Scientists can now probe more deeply into new and regenerated production, and directly identify major agents of these processes using new molecular techniques. This project will quantify new and regenerated production in a coastal ecosystem, illuminating the predominant compounds involved. Rates of primary production, nitrate, ammonium and urea assimilation, N2 fixation, and nitrification will be determined in the upper water column in concert with monthly SPOT cruises. In tandem, two stable isotope probing (SIP) approaches (conventional SIP for nitrate, ammonium and urea uptake coupled to high throughput sequencing and microarray based Chip-SIP for N2 fixation) will be used to directly identify the major agents involved in these processes, along with the uptake of 13C-urea into nitrifier biomass. The following two hypotheses will be tested:</p>
<p>1. N2 fixation is a substantial source of new N in coastal waters of Southern California supporting export production.</p>
<p>2. Forms of dissolved organic N, and specifically urea, can be substrates for nitrification and contribute substantially to regenerated production.</p>
<p>See the related project "<a href="http://www.bco-dmo.org/project/560420" target="_blank">Direct Identification and Characterization of Marine Heterotrophic Nitrogen Fixers by Stable Isotope Probing</a>", funded by OCE-1341178, that involved novel stable isotope probing (SIP) methods.</p>
N-SPOT
largerWorkCitation
project
eng; USA
oceans
an Pedro Ocean Time-series (SPOT)
-118.4
-118.4
33.55
33.55
2014-09-10
2016-07-12
Coastal Waters of Southern California, San Pedro Ocean Time-series (SPOT), located 17 km offshore between Los Angeles Harbor and Catalina Island
0
BCO-DMO catalogue of parameters from Primary production from the San Pedro Ocean Time-series (SPOT) measured between 2014 to 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/821554.rdf
Name: date
Units: unitless
Description: Date. Format: yyyy-mm-dd
http://lod.bco-dmo.org/id/dataset-parameter/821555.rdf
Name: depth
Units: meters (m)
Description: Depth
http://lod.bco-dmo.org/id/dataset-parameter/821556.rdf
Name: pp_1
Units: nanomoles per liter per day (nmol L-1 d-1)
Description: Primary production
http://lod.bco-dmo.org/id/dataset-parameter/821557.rdf
Name: pp_2
Units: nanomoles per liter per day (nmol L-1 d-1)
Description: Primary production
http://lod.bco-dmo.org/id/dataset-parameter/821558.rdf
Name: pp_3
Units: nanomoles per liter per day (nmol L-1 d-1)
Description: Primary production
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://www.bco-dmo.org/dataset/821539/data/download
download
onLine
dataset
<p>From 2014 to 2015, water samples were collected using a 12 x 12 L Niskin bottle rosette equipped with a conductivity, temperature, and density (CTD) instrument package (SBE 9plus, Sea-Bird Electronics, Bellevue, Washington, USA), including dissolved oxygen (SBE 43) and photosynthetically available radiation (PAR, LI-COR, Biospherical Instruments Inc., San Diego, California, USA) sensors. Due to CTD failure, samples collected in 2015 and 2016 were collected primarily using manually triggered Go-Flo bottles and depths were chosen primarily using a profiling natural fluorometer (PNF) system as well as secchi disk.</p>
<p>Rates of primary production were measured by quantifying the rate of CO2 fixation via H¹³CO₃ uptake. Samples were analyzed using an IsoPrime continuous flow isotope ratio mass spectrometer. Quadruplicate samples were collected and amended to a final concentration of ~25 µmol L-1 of H¹³CO₃. Incubations were carried out for 24 hrs at ambient temperature and light.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- changed date format to yyyy-mm-dd;<br />
- replaced blanks (no data) with "nd";<br />
- rounded primary production values to 1 decimal place.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Go-Flo bottles
Go-Flo bottles
PI Supplied Instrument Name: Go-Flo bottles Instrument Name: GO-FLO Bottle Instrument Short Name:GO-FLO Instrument Description: GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/
Niskin bottle
Niskin bottle
PI Supplied Instrument Name: Niskin bottle Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
IsoPrime continuous flow isotope ratio mass spectrometer
IsoPrime continuous flow isotope ratio mass spectrometer
PI Supplied Instrument Name: IsoPrime continuous flow isotope ratio mass spectrometer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
profiling natural fluorometer
profiling natural fluorometer
PI Supplied Instrument Name: profiling natural fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
SBE 9plus
SBE 9plus
PI Supplied Instrument Name: SBE 9plus PI Supplied Instrument Description:From 2014 to 2015, water samples were collected using a 12 x 12 L Niskin bottle rosette equipped with a conductivity, temperature, and density (CTD) instrument package (SBE 9plus, Sea-Bird Electronics, Bellevue, Washington, USA), including dissolved oxygen (SBE 43) and photosynthetically available radiation (PAR, LI-COR, Biospherical Instruments Inc., San Diego, California, USA) sensors. Due to CTD failure, samples collected in 2015 and 2016 were collected primarily using manually triggered Go-Flo bottles and depths were chosen primarily using a profiling natural fluorometer (PNF) system as well as secchi disk. Instrument Name: CTD Sea-Bird 9 Instrument Short Name:CTD SBE 9 Instrument Description: The Sea-Bird SBE 9 is a type of CTD instrument package. The SBE 9 is the Underwater Unit and is most often combined with the SBE 11 Deck Unit (for real-time readout using conductive wire) when deployed from a research vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorometer, altimeter, etc.). Note that in most cases, it is more accurate to specify SBE 911 than SBE 9 since it is likely a SBE 11 deck unit was used. more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
secchi disk
secchi disk
PI Supplied Instrument Name: secchi disk Instrument Name: Secchi Disc Instrument Short Name:Secchi Disc Instrument Description: Typically, a 16 inch diameter white/black quadrant disc used to measure water optical clarity Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0430/
Cruise: N-SPOT_Yellowfin_Cruises
N-SPOT_Yellowfin_Cruises
R/V Yellowfin
R/V Yellowfin
vessel
N-SPOT_Yellowfin_Cruises
Douglas G. Capone
University of Southern California
R/V Yellowfin
R/V Yellowfin
vessel