http://lod.bco-dmo.org/id/dataset/741219
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-07-24
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Fatty acid concentration extracted from A. pisonii eggs from three habitats along the Florida eastern coast, 2016
2018-07-16
publication
2018-07-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-07-16
publication
https://doi.org/10.26008/1912/bco-dmo.741219.1
Dr Blaine D. Griffen
University of South Carolina
principalInvestigator
Zachary J. Cannizzo
University of South Carolina
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
documentDigital
Cite this dataset as: Griffen, B. D., Cannizzo, Z. J. (2018) Fatty acid concentration extracted from A. pisonii eggs from three habitats along the Florida eastern coast, 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-07-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.741219.1 [access date]
Egg fatty acid concentration of A. pisonii from three habitats Dataset Description: <p>This dataset includes concentrations of fatty acids from eggs of mangrove tree crabs, A. pisonii, from three habitats: mangrove, saltmarsh and mangrove collected along the eastern Florida coast in 2016.</p> Acquisition Description: <p>We collected 10 ovigerous females with stage-1 non-eyed eggs by hand and from each habitat (mangrove, salt marsh, dock) during the week preceding the full moon of each of five consecutive months throughout the A. pisonii reproductive season. Crabs were immediately placed on dry ice and stored at -80oC until dissection at which time the size and gut-width were determined and the whole egg clutch was carefully removed from the pleopods. The egg clutch was then freeze-fried and stored at -80oC until analysis.</p>
<p>Lipids were extracted from eggs using a modified Folch extraction and the fatty acids were then methylated. We then analyzed the methylated fatty acids via gas chromatography-mass spectrometry using an Agilent 6890 GC 5975 Mass Spectrometer fitted with a Restek 30m FAMEWAX column. The concentration of each FA was then determined from a diluton curve derived from a Supelco 37 Fatty Acid mix. The FA peak areas were first normalized to recovery standard then concentrations were determined using the regression curves of the external standards. This concentration was then corrected for the volume of the sample to obtain the mass of the fatty acid extracted from the egg.</p>
<p>Gut-width:carapace-width ratio was calculated as the ratio of the size of the gut of the crab and the size of the crab.</p>
<p>The mass of Omega-3 Fatty Acids was determined by combining the masses of Alpha Linoleic Acid, Eicosatrienoic Acid, Eicosapentaenoic Acid, and Docosahexaenoic Acid.</p>
<p>The mass of Omega-6 Fatty Acids was determined by combining the masses of cis-Linoleic Acid, trans-Linoleic Acid, Eicosadienoic Acid, Dihomo-Gamma-Linolenic Acid, Arachidonic Acid, and Docosahexaenoic Acid.</p>
<p>The mass of the highly-unsaturated Fatty Acids was determined by combining the masses of Eicosapentaenoic Acid, Arachidonic Acid, and Docosahexaenoic Acid.</p>
<p>The mass of odd-numbered Fatty Acids was determined by combining the masses of C15:0 fatty acid, C15:1 fatty acid, C17:0 fatty acid, and C17:1 fatty acid.</p>
<p>The mass of each fatty acid extracted from the eggs was divided by the mass of the eggs from which fatty acids were extracted to obtain the concentration of that fatty acid in the eggs as the proportion of the egg mass.</p>
<p>Locations, Florida East Coast:<br />
Round Island Park: 27o33'33"N 80o19'53"W<br />
Pepper Park: 27o29'42'N 80o18'12"W<br />
North Causeway Park: 27o28'28"N 80o19'12"W<br />
Oslo Road: 27o35'14"N 80o21'55"W<br />
Anastasia State Park: 29o52'40"N 81o16'32"W<br />
Guana-Tolomato-Matanzas NERR: 30o0'49"N 81o20'42"W<br />
Vilano Inlet: 29o55'16"N 81o17'57"W<br />
Palm Valley/Nocatee Canoe Launch: 30o07'57"N 81o23'08"W<br />
St. Augustine Yacht Club: 29o53'09"N 81o17'08"W<br />
Boating Club Road: 29o56'34"N 81o18'31"W</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1129166 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1129166
completed
Dr Blaine D. Griffen
University of South Carolina
801-422-5930
Provo
UT
84602
USA
blaine_griffen@byu.edu
pointOfContact
Zachary J. Cannizzo
University of South Carolina
815-353-1457
2524 Kiawah Ave.
Columbia
SC
29205
zac.cannizzo@gmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample
Habitat
Site
Year
Month
CW
GW_CW
Egg_ug
C14_0
C14_1
C15_0
C15_1
C16_0
C16_1
C17_0
C17_1
C18_0
C18_1n9
cLA
tLA
GLA
ALA
C20_0
C20_1n9
EicoA
DGLA
AA
ETE
EPA
DHA
O3
O6
O3_O6
EPA_DHA
HUFA
OFA
C14_0_Egg
C14_1_Egg
C15_0_Egg
C15_1_Egg
C16_0_Egg
C16_1_Egg
C17_0_Egg
C17_1_Egg
C18_0_Egg
C18_1n9_Egg
cLA_Egg
tLA_Egg
GLA_Egg
ALA_Egg
C20_0_Egg
C20_1n9_Egg
EicoA_Egg
DGLA_Egg
AA_Egg
ETE_Egg
EPA_Egg
DHA_Egg
O3_Egg
O6_Egg
HUFA_Egg
OFA_Egg
Agilent 6890 GC 5975 Mass Spectrometer
theme
None, User defined
sample identification
site description
site
year
month of year
width
no standard parameter
lipids in biota
featureType
BCO-DMO Standard Parameters
Mass Spectrometer
Scale
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Linking Variation in Metabolic Processes as a Key to Prediction
https://www.bco-dmo.org/project/562104
Linking Variation in Metabolic Processes as a Key to Prediction
<p><em>Description from NSF award abstract:</em><br />
A major goal of biological and ecological sciences is to understand natural systems well enough to predict how species and populations will respond to a rapidly changing world (i.e., climate change, habitat loss, etc.). A population under any conditions will grow, shrink, or disappear altogether depending on how efficiently individuals consume resources (food), utilize that food metabolically, and eventually reproduce. However, making accurate predictions based on these metabolic processes is complicated by the realities that each species has different resource requirements and that no two individuals within a species are exactly alike. Rather, individuals vary and this variation, both within and across species, is central to many ecological and evolutionary processes. Developing the ability to predict responses of biological systems to a changing world therefore requires a mechanistic understanding of variation. The goal of this project is to improve this mechanistic understanding by examining variation within a metabolic context across a range of species that have a spectrum of commonly-seen resource requirements. Further, the work capitalizes on a unique biological characteristic of this group of species that allows control and manipulation of individual reproduction, facilitating experimental study of the mechanistic links between variation in individual consumption, metabolism, and reproduction. The foundation this research is a combination of field measurements and laboratory experiments using both well-established and newly-developed techniques to quantify these links. The result will be a quantitative framework to predict how individuals will respond reproductively to changes in resource use. Because of the close link between individual reproduction and population dynamics, this research will contribute substantially to predictions in population dynamics under realistic conditions where individuals use more than a single resource, and improve the prediction of responses to current and future ecological changes.</p>
<p><strong>The following publications and data resulted from this project:</strong></p>
<p>Belgrad, B. and B. Griffen. 2016. Predator-prey interactions mediated by prey personality and predator identity.<em>Proc. Roy. Soc. B</em>: In Review. [2016-01-20]<br /><a href="http://www.bco-dmo.org/dataset/635735">P. herbstii mortality data</a>: Mortality of crabs when exposed to either a single blue crab, toadfish, or no predator for a week<br /><a href="http://www.bco-dmo.org/dataset/635805">P. herbstii personality data</a>: Refuge use of crabs when exposed to predator odor cues from either blue crabs, toadfish, or control of no cue<br /><a href="http://www.bco-dmo.org/dataset/635833">P. herbstii predator behavior data</a>: Refuge use and mobility of blue crabs and toadfish while in mesocosms for a week - behavior measured during two days.</p>
<p>Belgrad, B. and B. Griffen. 2016. The influence of dietary shifts on fitness of the blue crab, <em>Callinectes sapidus</em>. <em>PloS One. DOI: <a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145481" target="_blank">10.1371/journal.pone.0145481</a>.</em><br /><a href="http://www.bco-dmo.org/dataset/630014">Blue crab activity</a>: Activity of crabs fed different diets over a summer<br /><a href="http://www.bco-dmo.org/dataset/629934">Blue crab egg size</a>: Volume of eggs for crabs fed different diets<br /><a href="http://www.bco-dmo.org/dataset/629856">Blue crab hepatopancreas index (HSI)</a>: Weight of hepatopancreas for crabs fed different diets<br /><a href="http://www.bco-dmo.org/dataset/629878">Blue crab hepatopancreas lipid content</a>: Hepatopancreas lipid content of crabs fed different diets<br /><a href="http://www.bco-dmo.org/dataset/629830">Blue crab reproductive tissue analysis (GSI)</a>: Gonadosomatic index of blue crabs on various diets<br /><a href="http://www.bco-dmo.org/dataset/629965">Blue crab survival</a>: Blue crab survival data during the dietary study</p>
<p>Knotts ER, Griffen BD. 2016. Individual movement rates are sufficient to determine and maintain dynamic spatial positioning within <em>Uca pugilator</em> herds. <em>Behavioral Ecology and Sociobiology</em> 70:639-646<br /><a href="http://www.bco-dmo.org/dataset/642465" target="_blank">Uca pugilator: behavior change with carapace marking</a>: Search space behavior due to carapace treatment (control, nail polish, and food dye)<br /><a href="http://www.bco-dmo.org/dataset/642057" target="_blank">Uca pugilator: field spatial position</a>: Assessment of individual's position within a herd at 3 min. intervals; for proportion of time found at edge of herd<br /><a href="http://www.bco-dmo.org/dataset/642081" target="_blank">Uca pugilator: herd position proportion</a>: Individual's proportion of time spent in an edge/alone position among a herd<br /><a href="http://www.bco-dmo.org/dataset/642447" target="_blank">Uca pugilator: search space distribution</a>: Search space that crabs traveled; to evaluate the sample's distribution of exploratory behavior</p>
<p>Belgrad, B. and B. Griffen. 2015. Rhizocephalan infection modifies host food consumption by reducing host activity levels. <em>Journal of Experimental Marine Biology and Ecology</em>. 466: 70-75.<br /><a href="http://www.bco-dmo.org/dataset/638671">E. depressus digestion time</a> : Time taken for food to pass through gut of flat-backed mud crabs infected by a parasite<br /><a href="http://www.bco-dmo.org/dataset/638697">E. depressus metabolism</a>: Respiration rate of infected/uninfected flat-backed mud crabs<br /><a href="http://www.bco-dmo.org/dataset/638726">E. depressus reaction time to prey</a>: Time taken for infected/uninfected flat-backed mud crabs to react to the presence of prey</p>
<p>Blakeslee, A.M., C.L. Keogh, A.E. Fowler, B. Griffen. 2015. Assessing the effects of trematode infection on invasive green crabs in eastern North America. <em>PLOS One</em> 10(6): e0128674.(<a href="http://dmoserv3.bco-dmo.org/data_docs/Variation_Metabolic_Processes/Blakeslee_etal_PlosOne_2015.pdf" target="_blank">pdf</a>)<br /><a href="http://www.bco-dmo.org/dataset/642488" target="_blank">Carcinus: hemocyte density</a>: Counts of circulating hemocyte density in Carcinus maenas<br /><a href="http://www.bco-dmo.org/dataset/642804" target="_blank">Carcinus: parasites physiology behavior</a>: Behavior and physiology of Carcinus maenas infected with trematode parasite</p>
<p>Griffen BD, Norelli AP (2015) Spatially variable habitat quality contributes to within-population variation in reproductive success. <em>Ecology and Evolution</em> 5:1474-1483.<br /><a href="http://www.bco-dmo.org/dataset/648291">P. herbstii diet: sampling site characteristics (Eco-Evo 2015)</a><br /><a href="http://www.bco-dmo.org/dataset/648300">P. herbstii diet: body measurements (Eco-Evo 2015)</a><br /><a href="http://www.bco-dmo.org/dataset/648281">P. herbstii diet & reproduction (Eco-Evo 2015)<br />
P. herbstii: collection sites (Ecol-Evol 2015)</a></p>
<p>Griffen BD, Riley ME (2015) Potential impacts of invasive crabs on one life history strategy of native rock crabs in the Gulf of Maine. Biological Invasions 17:2533-2544.<br /><a href="http://www.bco-dmo.org/dataset/644899">Cancer consumption and reproduction (Bio.Inv. 2015)</a>: Lab experiment linking dietary consumption and reproduction</p>
<p>Griffen BD, Vogel M, Goulding L, Hartman R (2015) Energetic effects of diet choice by invasive Asian shore crabs: implications for persistence when prey are scarce. <em>Marine Ecology Progress Series</em> 522:181-192.<br /><a href="http://www.bco-dmo.org/dataset/648239">Hemigrapsus diet 1 (MEPS 2015)</a><br /><a href="http://www.bco-dmo.org/dataset/648272">Hemigrapsus diet 2 (MEPS 2015)</a></p>
<p>Hogan and Griffen (2014). The Dietary And Reproductive Consequences Of Fishery-Related Claw Removal For The Stone Crab <em>Menippe</em> Spp. Journal of Shellfish Research, Vol. 33, No. 3, 795–804.<br /><a href="http://www.bco-dmo.org/dataset/638569">Stone crab: 052012-DietChoiceExp1</a>: Prey choice for 2-clawed and 1-clawed Stone Crabs (Menippe spp.)<br /><a href="http://www.bco-dmo.org/dataset/638622">Stone crab: 052012-LongTermConsumption</a>: Long-term consuption for 2-clawed and 1-clawed Stone Crabs (Menippe spp.), summer of 2012<br /><a href="http://www.bco-dmo.org/dataset/638602">Stone crab: 062013-DietChoiceExp2</a>: Prey choice for 2-clawed and 1-clawed Stone Crabs (Menippe spp.)<br /><a href="http://www.bco-dmo.org/dataset/638645">Stone crab: 062013-PreySizeSelection</a>: Prey Size selection ranking for 2-clawed and 1-clawed Stone Crabs (Menippe spp.)</p>
<p>Riley M, Johnston CA, Feller IC, and Griffen B. 2014. Range expansion of <em>Aratus pisonii </em>(mangrove tree crab) into novel vegetative habitats. <em>Southeastern Naturalist </em>13(4): 43-38<br /><a href="http://www.bco-dmo.org/dataset/639205">A. pisonii: range expansion</a>: Aratus pisonii survey in native mangrove and novel salt marsh habitats</p>
<p>Riley M, Vogel M, Griffen B. 2014. Fitness-associated consequences of an omnivorous diet for the mangrove tree crab <em>Aratus pisonii</em>. <em>Aquatic Biology </em>20:35-43, DOI: 10.3354/ab00543<br /><a href="http://www.bco-dmo.org/dataset/639095">A. pisonii: fitness and diet</a>: Impact of diet variation on physiological and reproductive condition of A. pisonii</p>
<p>Toscano BJ, Newsome B, Griffen BD (2014) Parasite modification of predator functional response. Oecologia 175:345-352b<br /><a href="http://www.bco-dmo.org/dataset/638907">E. depressus - parasite and feeding (Oecologia, 2014)</a>: Feeding with and without parasitic barnacle infection<br /><a href="http://www.bco-dmo.org/dataset/638930">E. depressus - parasite and prey handling (Oecologia, 2014)</a>: Food handling with and without parasitic barnacle infection<br /><a href="http://www.bco-dmo.org/dataset/638956">E. depressus - parasite study - field survey (Oecologia, 2014)</a>: Parasitised field survey</p>
<p>Toscano BJ, Griffen BD (2014) Trait-mediated functional responses: predator behavioural type mediates prey consumption.<em>Journal of Animal Ecology</em> 83:1469-1477<br /><a href="http://www.bco-dmo.org/dataset/638884">P. herbstii - activity and feeding (JAE, 2014)</a>: Activity level and feeding with and without predator cue</p>
<p>Toscano BJ, Gatto J, Griffen BD (2014) Effects of predation threat on repeatability of individual crab behavior revealed by mark recapture. <em>Behavioral Ecology and Sociobiology</em> 68:519-527<br /><a href="http://www.bco-dmo.org/dataset/638853">P. herbstii - recapture behavior (BESB, 2014)</a>: Mud crabs refuge use and activity level - initial measurements<br /><a href="http://www.bco-dmo.org/dataset/638825">P. herbstii - refuge use (BESB, 2014)</a>: Effect of predation threat on repeatability of individual crab behavior revealed by mark-recapture</p>
<p>Griffen BD, Altman I, Bess BM, Hurley J, Penfield A (2012) The role of foraging in the success of invasive species. Biological Invasions. 14:2545-2558<br /><a href="http://www.bco-dmo.org/dataset/644932">Hemigrapsus seasonal diet (Bio.Inv. 2012)</a>: Percent herbivory and gut fullness for Hemigrapsus sanguineus at different times of year</p>
<p>Griffen BD, Toscano B, Gatto J (2012) The role of intraspecific trait variation in mediating indirect interactions. Ecology 93:1935-1943<br /><a href="http://www.bco-dmo.org/dataset/644846">P. herbstii refuge use (Ecology, 2012)</a>: Proportion of time that Panopeus herbstii spent using refuge habitats in a lab experiment<br /><a href="http://www.bco-dmo.org/dataset/644883">P. herbstii: Field personality distribution (Ecology, 2012)</a>: Field distribution of personality types in the mud crab Panopeus herbstii relative to tidal height<br /><a href="http://www.bco-dmo.org/dataset/644867">P. herbstii: Trait mediated indirect effect (Ecology, 2012)</a>: Influence of refuge use by the mud crab Panopeus herbstii on consumption of bivalves</p>
<p>Riley ME, Griffen BD (2017) Habitat-specific differences alter traditional biogeographic patterns of life history in a climate-change induced range expansion. PLOS One 12(5):e0176263<br /><a href="http://www.bco-dmo.org/dataset/639282">A. pisonii: egg size</a>: Comparing egg size in Aratus pisonii populations from mangrove and salt marsh habitats<br /><a href="http://www.bco-dmo.org/dataset/639229">A. pisonii: fecundity</a>: Determining fecundity of Aratus pisonii populations in mangrove and salt marsh habitats<br /><a href="http://www.bco-dmo.org/dataset/639320">A. pisonii: larval starvation resistance</a>: Comparing larval quality in Aratus pisonii populations from mangrove and salt marsh habitats<br /><a href="http://www.bco-dmo.org/dataset/639250">A. pisonii: latitudinal body size</a>: Survey examining latitudinal body size patterns in Aratus pisonii<br /><a href="http://www.bco-dmo.org/dataset/639301">A. pisonii: predation</a>: Comparing predation pressure on Aratus pisonii in mangrove and salt marsh habitats<br /><a href="http://www.bco-dmo.org/dataset/639267">A. pisonii: reproductive effort</a>: Survey comparing Aratus pisonii reproductive effort in native and novel habitats<br /><a href="http://www.bco-dmo.org/dataset/639335">A. pisonii: herbivory</a>: Relationship between leaf herbivory, tree characteristics, and refuge availability<br /><a href="http://www.bco-dmo.org/dataset/639360">A. pisonii: mangrove tree survey</a>: Mangrove tree distribution and characteristics in a dwarf mangrove system</p>
<p>Cannizzo ZJ, Dixon SR & Griffen BD (2018). An anthropogenic habitat within a suboptimal colonized ecosystem provides improved conditions for a range-shifting species. Ecology and Evolution, 8(3):1524-1533.<br /><a href="https://www.bco-dmo.org/dataset/740014">A. pisonii: behavior</a>: Proportion of time the mangrove tree crab Aratus pisonii spent in different behaviors related to diet and energy storage<br /><a href="https://www.bco-dmo.org/dataset/740137">A. pisonii: dock-marsh thermal</a>: Thermal readings from under a dock and in a nearby salt marsh<br /><a href="https://www.bco-dmo.org/dataset/741059">A. pisonii: sun-shade</a>: Proportion of time that mangrove tree crab Aratus pisonii spent in sun and shade in three habitats, 2015-2016.<br /><a href="https://www.bco-dmo.org/dataset/741032">A. pisonii: thermal picture</a>: Thermal condition of A. pisonii in three habitats: under dock, mangroves, saltmarsh</p>
Variation in Metabolic Processes
largerWorkCitation
project
eng; USA
biota
oceans
-81.38556
-80.28611
27.43
30.1325
2016-06-14
2016-10-13
0
BCO-DMO catalogue of parameters from Fatty acid concentration extracted from A. pisonii eggs from three habitats along the Florida eastern coast, 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/741231.rdf
Name: Sample
Units: unitless
Description: individual ID number given to each crab
http://lod.bco-dmo.org/id/dataset-parameter/741232.rdf
Name: Habitat
Units: unitless
Description: The habitat where the crab was collected and observed.
http://lod.bco-dmo.org/id/dataset-parameter/741233.rdf
Name: Site
Units: unitless
Description: Site of observation/collection. RI= Round Island Park; PP=Pepper Park; NC=North Causeway Park; Oslo=Oslo Road; ANA=Anastasia State Park; GTM= Guana-Tolomato-Matanzas NERR; VM=Vilano Inlet; PV= Palm Valley/Nocatee Canoe Launch; YC=St. Augustine Yacht Club; BC=Boating Club Road
http://lod.bco-dmo.org/id/dataset-parameter/741234.rdf
Name: Year
Units: unitless
Description: Year of collection
http://lod.bco-dmo.org/id/dataset-parameter/741235.rdf
Name: Month
Units: unitless
Description: Number of month (1-12) that crab was collected
http://lod.bco-dmo.org/id/dataset-parameter/741236.rdf
Name: CW
Units: millimeters
Description: Size of crab; measured as carapace-width
http://lod.bco-dmo.org/id/dataset-parameter/741237.rdf
Name: GW_CW
Units: unitless
Description: gut-width:carapace-width ratio
http://lod.bco-dmo.org/id/dataset-parameter/741238.rdf
Name: Egg_ug
Units: micrograms
Description: Mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741239.rdf
Name: C14_0
Units: micrograms
Description: Mass of C14:0 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741240.rdf
Name: C14_1
Units: micrograms
Description: Mass of C14:1 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741241.rdf
Name: C15_0
Units: micrograms
Description: Mass of C15:0 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741242.rdf
Name: C15_1
Units: micrograms
Description: Mass of C15:1 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741243.rdf
Name: C16_0
Units: micrograms
Description: Mass of C16:0 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741244.rdf
Name: C16_1
Units: micrograms
Description: Mass of C16:1 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741245.rdf
Name: C17_0
Units: micrograms
Description: Mass of C17:0 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741246.rdf
Name: C17_1
Units: micrograms
Description: Mass of C17:1 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741247.rdf
Name: C18_0
Units: micrograms
Description: Mass of C18:0 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741248.rdf
Name: C18_1n9
Units: micrograms
Description: Mass of C18:1n9 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741249.rdf
Name: cLA
Units: micrograms
Description: Mass of cis-Linoleic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741250.rdf
Name: tLA
Units: micrograms
Description: Mass of trans-Linoleic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741251.rdf
Name: GLA
Units: micrograms
Description: Mass of Gamma-Linolenic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741252.rdf
Name: ALA
Units: micrograms
Description: Mass of Alpha Linoleic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741253.rdf
Name: C20_0
Units: micrograms
Description: Mass of C20:0 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741254.rdf
Name: C20_1n9
Units: micrograms
Description: Mass of C20:1n9 fatty acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741255.rdf
Name: EicoA
Units: micrograms
Description: Mass of Eicosadienoic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741256.rdf
Name: DGLA
Units: micrograms
Description: Mass of Dihomo-Gamma-Linolenic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741257.rdf
Name: AA
Units: micrograms
Description: Mass of Arachidonic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741258.rdf
Name: ETE
Units: micrograms
Description: Mass of Eicosatrienoic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741259.rdf
Name: EPA
Units: micrograms
Description: Mass of Eicosapentaenoic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741260.rdf
Name: DHA
Units: micrograms
Description: Mass of Docosahexaenoic Acid extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741261.rdf
Name: O3
Units: micrograms
Description: Mass of Omega-3 Fatty Acids extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741262.rdf
Name: O6
Units: micrograms
Description: Mass of Omega-6 Fatty Acids extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741263.rdf
Name: O3_O6
Units: unitless
Description: Ratio of the masses of the Omega-3 and Omega-6 Fatty Acids extracted from eggs
http://lod.bco-dmo.org/id/dataset-parameter/741264.rdf
Name: EPA_DHA
Units: unitless
Description: Ratio of the masses of Eicosapentaenoic Acid and Docosahexaenoic Acid extracted from eggs
http://lod.bco-dmo.org/id/dataset-parameter/741265.rdf
Name: HUFA
Units: micrograms
Description: Mass of the highly-unsaturated Fatty Acids extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741266.rdf
Name: OFA
Units: micrograms
Description: Mass of the odd-numbered Fatty Acids extracted from eggs.
http://lod.bco-dmo.org/id/dataset-parameter/741267.rdf
Name: C14_0_Egg
Units: unitless
Description: Concentration of C14:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741268.rdf
Name: C14_1_Egg
Units: unitless
Description: Concentration of C14:1 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741269.rdf
Name: C15_0_Egg
Units: unitless
Description: Concentration of C15:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741270.rdf
Name: C15_1_Egg
Units: unitless
Description: Concentration of C15:1 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741271.rdf
Name: C16_0_Egg
Units: unitless
Description: Concentration of C16:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741272.rdf
Name: C16_1_Egg
Units: unitless
Description: Concentration of C16:1 fatty as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741273.rdf
Name: C17_0_Egg
Units: unitless
Description: Concentration of C17:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741274.rdf
Name: C17_1_Egg
Units: unitless
Description: Concentration of C17:1 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741275.rdf
Name: C18_0_Egg
Units: unitless
Description: Concentration of C18:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741276.rdf
Name: C18_1n9_Egg
Units: unitless
Description: Concentration of C18:1n9 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted..
http://lod.bco-dmo.org/id/dataset-parameter/741277.rdf
Name: cLA_Egg
Units: unitless
Description: Concentration of cis-Linoleic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741278.rdf
Name: tLA_Egg
Units: unitless
Description: Concentration of trans-Linoleic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741279.rdf
Name: GLA_Egg
Units: unitless
Description: Concentration of Gamma-Linolenic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741280.rdf
Name: ALA_Egg
Units: unitless
Description: Concentration of Alpha Linoleic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741281.rdf
Name: C20_0_Egg
Units: unitless
Description: Concentration of C20:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741282.rdf
Name: C20_1n9_Egg
Units: unitless
Description: Concentration of C20:1n9 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741283.rdf
Name: EicoA_Egg
Units: unitless
Description: Concentration of Eicosadienoic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741284.rdf
Name: DGLA_Egg
Units: unitless
Description: Concentration of Dihomo-Gamma-Linolenic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741285.rdf
Name: AA_Egg
Units: unitless
Description: Concentration of Arachidonic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741286.rdf
Name: ETE_Egg
Units: unitless
Description: Concentration of Eicosatrienoic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741287.rdf
Name: EPA_Egg
Units: unitless
Description: Concentration of Eicosapentaenoic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741288.rdf
Name: DHA_Egg
Units: unitless
Description: Concentration of Docosahexaenoic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741289.rdf
Name: O3_Egg
Units: unitless
Description: Concentration of Omega-3 Fatty Acids as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741290.rdf
Name: O6_Egg
Units: unitless
Description: Concentration of Omega-6 Fatty Acids as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741291.rdf
Name: HUFA_Egg
Units: unitless
Description: Concentration of the highly-unsaturated Fatty Acids as the proportion of the mass of the eggs from which the fatty acids were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741292.rdf
Name: OFA_Egg
Units: unitless
Description: Concentration of the odd-numbered Fatty Acids as the proportion of the mass of the eggs from which the fatty acids were extracted.
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://www.bco-dmo.org/dataset/741219/data/download
download
onLine
dataset
<p>We collected 10 ovigerous females with stage-1 non-eyed eggs by hand and from each habitat (mangrove, salt marsh, dock) during the week preceding the full moon of each of five consecutive months throughout the A. pisonii reproductive season. Crabs were immediately placed on dry ice and stored at -80oC until dissection at which time the size and gut-width were determined and the whole egg clutch was carefully removed from the pleopods. The egg clutch was then freeze-fried and stored at -80oC until analysis.</p>
<p>Lipids were extracted from eggs using a modified Folch extraction and the fatty acids were then methylated. We then analyzed the methylated fatty acids via gas chromatography-mass spectrometry using an Agilent 6890 GC 5975 Mass Spectrometer fitted with a Restek 30m FAMEWAX column. The concentration of each FA was then determined from a diluton curve derived from a Supelco 37 Fatty Acid mix. The FA peak areas were first normalized to recovery standard then concentrations were determined using the regression curves of the external standards. This concentration was then corrected for the volume of the sample to obtain the mass of the fatty acid extracted from the egg.</p>
<p>Gut-width:carapace-width ratio was calculated as the ratio of the size of the gut of the crab and the size of the crab.</p>
<p>The mass of Omega-3 Fatty Acids was determined by combining the masses of Alpha Linoleic Acid, Eicosatrienoic Acid, Eicosapentaenoic Acid, and Docosahexaenoic Acid.</p>
<p>The mass of Omega-6 Fatty Acids was determined by combining the masses of cis-Linoleic Acid, trans-Linoleic Acid, Eicosadienoic Acid, Dihomo-Gamma-Linolenic Acid, Arachidonic Acid, and Docosahexaenoic Acid.</p>
<p>The mass of the highly-unsaturated Fatty Acids was determined by combining the masses of Eicosapentaenoic Acid, Arachidonic Acid, and Docosahexaenoic Acid.</p>
<p>The mass of odd-numbered Fatty Acids was determined by combining the masses of C15:0 fatty acid, C15:1 fatty acid, C17:0 fatty acid, and C17:1 fatty acid.</p>
<p>The mass of each fatty acid extracted from the eggs was divided by the mass of the eggs from which fatty acids were extracted to obtain the concentration of that fatty acid in the eggs as the proportion of the egg mass.</p>
<p>Locations, Florida East Coast:<br />
Round Island Park: 27o33'33"N 80o19'53"W<br />
Pepper Park: 27o29'42'N 80o18'12"W<br />
North Causeway Park: 27o28'28"N 80o19'12"W<br />
Oslo Road: 27o35'14"N 80o21'55"W<br />
Anastasia State Park: 29o52'40"N 81o16'32"W<br />
Guana-Tolomato-Matanzas NERR: 30o0'49"N 81o20'42"W<br />
Vilano Inlet: 29o55'16"N 81o17'57"W<br />
Palm Valley/Nocatee Canoe Launch: 30o07'57"N 81o23'08"W<br />
St. Augustine Yacht Club: 29o53'09"N 81o17'08"W<br />
Boating Club Road: 29o56'34"N 81o18'31"W</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
-&nbsp;reduced decimal precision of: GW_CW from 9 to 3 places</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Agilent 6890 GC 5975 Mass Spectrometer
Agilent 6890 GC 5975 Mass Spectrometer
PI Supplied Instrument Name: Agilent 6890 GC 5975 Mass Spectrometer Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
PI Supplied Instrument Name: Instrument Name: Scale Instrument Short Name:Scale Instrument Description: An instrument used to measure weight or mass. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/