http://lod.bco-dmo.org/id/dataset/733238
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-04-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions
2018-04-11
publication
2018-04-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-03
publication
https://doi.org/10.1575/1912/bco-dmo.733238.1
Dr Janet E Kubler
California State University Northridge
principalInvestigator
Dr Steve Dudgeon
California State University Northridge
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
documentDigital
Cite this dataset as: Kubler, J., Dudgeon, S. (2018) Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2018-04-11 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.733238.1 [access date]
Photosynthetic pigment concentrations in Plocamium cartilagineum trials 3-8 Dataset Description: <p>This dataset includes photosynthetic pigment concentrations in Plocamium cartilagineum grown under various temperatures and CO2 levels, from July 2014 to February 2015. The parameters reported are: the concentrations of Chlorophyll a, Phycoerythrin, Phycocyanin, Allophycocyanin, and total Phycobiliprotein, and the ratio of total Phycobiliprotein to Chlorophyll A.</p>
<p><strong>Related Datasets:</strong><br />
<a href="https://www.bco-dmo.org/dataset/731187">Plocamium carbon nitrogen and stable isotopes</a>: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726664">Plocamium culture carbonate chemistry</a>: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731237">Plocamium culture: seawater delta13C</a>: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731134">Plocamium cultures pH and temperature</a>: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731452">Plocamium exptl treatments summary</a>: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726452">Plocamium growth and biomass</a>: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726480">Plocamium: pH drift</a>: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)</p> Acquisition Description: <p><em>Plocamium </em><em>cartilagineum</em> was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.</p>
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Measurements of&nbsp;chlorophyll fluorescence of photosystem II were made using a pulse amplitude modulated (PAM) fluorometer in situ in culture pots. All samples were dark-adapted prior to measurement by placing a leaf clip in the closed position over the area to be sampled for 5 minutes prior to measurement. For fluorescence measurements, the fiber optic probe was placed directly on the leaf clip perpendicular to the sample. The leaf clip was then opened and a rapid light curve program function was initiated to measure fluorescence at each of 9 photon flux densities (PFD’s). PFD’s of the light curve generated by the fluorometer were calibrated using an external LiCor light meter. Following measurement, the leaf clip was removed and the sample remained in the culture chamber.&nbsp;</p>
<p>Data were downloaded from the Diving PAM using WinControl version 3 software for Diving PAM.</p>
<p>Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.</p>
<p>See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316198 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1316198
completed
Dr Janet E Kubler
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
janet.kubler@csun.edu
pointOfContact
Dr Steve Dudgeon
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
steve.dudgeon@csun.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Trial
Tank_ID
Plant_ID
pCO2
Temp
date
Week
ID
light
F
Fm
Yield
rETR
qP
qN
NPQ
Walz Underwater fluorometer - Diving-PAM, Heinz Walz, Effeltrich, Germany
theme
None, User defined
replicate
sample identification
pCO2
water temperature
date_local
time_point
PAR
fluorescence
Fv2Fm
no standard parameter
featureType
BCO-DMO Standard Parameters
Fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:NSF 10-530, FY 2010-FY2011NSF 12-500, FY 2012NSF 12-600, FY 2013NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
https://www.bco-dmo.org/project/2275
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
<p>Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, <i>Ulva</i> spp. and <i>Plocamium cartilagineum</i>, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in <i>Plocamium</i>, which relies solely on diffusive uptake of CO2, and populations of<i> Ulva</i> spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of<i> Ulva</i> spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on <i>Ulva</i> spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.</p>
Seaweed OA Resilience
largerWorkCitation
project
eng; USA
biota
oceans
-119
-118
33
34
2014-06-26
2015-02-12
Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
0
BCO-DMO catalogue of parameters from Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/733248.rdf
Name: Trial
Units: unitless
Description: Trial number of the experiment
http://lod.bco-dmo.org/id/dataset-parameter/733249.rdf
Name: Tank_ID
Units: unitless
Description: Identifying number of culture pot
http://lod.bco-dmo.org/id/dataset-parameter/733250.rdf
Name: Plant_ID
Units: unitless
Description: Plant ID number in culture pot
http://lod.bco-dmo.org/id/dataset-parameter/733251.rdf
Name: pCO2
Units: atm
Description: Partial pressure of CO2 in solution in culture pot
http://lod.bco-dmo.org/id/dataset-parameter/733252.rdf
Name: Temp
Units: degrees Celsius (°C)
Description: Temperature of seawater in culture pot
http://lod.bco-dmo.org/id/dataset-parameter/733253.rdf
Name: date
Units: unitless
Description: date of data collection formatted as yyyy-mm-dd
http://lod.bco-dmo.org/id/dataset-parameter/733254.rdf
Name: Week
Units: unitless
Description: Week number of given trial
http://lod.bco-dmo.org/id/dataset-parameter/733255.rdf
Name: ID
Units: unitless
Description: Unique identifier for every sample; concatenation of trial_tank ID_plant ID_week
http://lod.bco-dmo.org/id/dataset-parameter/733256.rdf
Name: light
Units: micromole photons/meter^2/second ( µmole photons . m^-2 . s^-1)
Description: Photosynthetically active radiation (PAR)
http://lod.bco-dmo.org/id/dataset-parameter/733257.rdf
Name: F
Units: unitless
Description: Relative fluorescence of photosystem II
http://lod.bco-dmo.org/id/dataset-parameter/733258.rdf
Name: Fm
Units: unitless
Description: Maximum fluorescence
http://lod.bco-dmo.org/id/dataset-parameter/733259.rdf
Name: Yield
Units: unitless
Description: Effective (or Optimal if dark-adapted) quantum yield. Calculated as Yield = (Fm - F)/Fm
http://lod.bco-dmo.org/id/dataset-parameter/733260.rdf
Name: rETR
Units: unitless
Description: Relative Electron Transport Rate
http://lod.bco-dmo.org/id/dataset-parameter/733261.rdf
Name: qP
Units: unitless
Description: Photochemical quenching
http://lod.bco-dmo.org/id/dataset-parameter/733262.rdf
Name: qN
Units: unitless
Description: Non-photochemical quenching expressed as a fraction of difference between Fm and minimum F
http://lod.bco-dmo.org/id/dataset-parameter/733263.rdf
Name: NPQ
Units: unitless
Description: Non-photochemical quenching normalized to Fm measured in the light
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://www.bco-dmo.org/dataset/733238/data/download
download
onLine
dataset
<p><em>Plocamium </em><em>cartilagineum</em> was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.</p>
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Measurements of&nbsp;chlorophyll fluorescence of photosystem II were made using a pulse amplitude modulated (PAM) fluorometer in situ in culture pots. All samples were dark-adapted prior to measurement by placing a leaf clip in the closed position over the area to be sampled for 5 minutes prior to measurement. For fluorescence measurements, the fiber optic probe was placed directly on the leaf clip perpendicular to the sample. The leaf clip was then opened and a rapid light curve program function was initiated to measure fluorescence at each of 9 photon flux densities (PFD’s). PFD’s of the light curve generated by the fluorometer were calibrated using an external LiCor light meter. Following measurement, the leaf clip was removed and the sample remained in the culture chamber.&nbsp;</p>
<p>Data were downloaded from the Diving PAM using WinControl version 3 software for Diving PAM.</p>
<p>Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.</p>
<p>See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- re-formatted date from dd.mm.yy to yyyy-mm-dd<br />
- reordered columns to correspond with good database practices</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Walz Underwater fluorometer - Diving-PAM, Heinz Walz, Effeltrich, Germany
Walz Underwater fluorometer - Diving-PAM, Heinz Walz, Effeltrich, Germany
PI Supplied Instrument Name: Walz Underwater fluorometer - Diving-PAM, Heinz Walz, Effeltrich, Germany PI Supplied Instrument Description:Used to measure chlorophyll fluorescence Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/