Endoplasmic reticulum targeted GFP reveals ER organization in tobacco NT-1 cells during cell division
Movie 1: Optical sectioning through a small part of a cell show a nucleus containing an invagination and a transnuclear channel, both of which contain further ER elements. (1.615Mb)
Movie 2: A time course showing changes in ER organization during cell division, over 60 minutes from preprophase through to telophase. (1.739Mb)
Movie 3: A time-course showing changes in ER organization during cell division, over 51 minutes from metaphase through to telophase. (851.4Kb)
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KeywordActin microfilaments; Cell division; Endoplasmic reticulum; Green fluorescent protein; Microtubules; Nuclear envelope; Nuclear invaginations
The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and invaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies, using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells.
Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Plant Physiology and Biochemistry, doi:10.1016/j.plaphy.2006.03.003.
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