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dc.contributor.authorSmith, Maria W.  Concept link
dc.contributor.authorHerfort, Lydie  Concept link
dc.contributor.authorFortunato, Caroline S.  Concept link
dc.contributor.authorCrump, Byron C.  Concept link
dc.contributor.authorSimon, Holly M.  Concept link
dc.date.accessioned2017-09-07T17:49:15Z
dc.date.available2017-09-07T17:49:15Z
dc.date.issued2017-03-20
dc.identifier.citationMicrobiologyOpen 6 (2017): e467en_US
dc.identifier.urihttps://hdl.handle.net/1912/9216
dc.description© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in MicrobiologyOpen 6 (2017): e467, doi:10.1002/mbo3.467.en_US
dc.description.abstractFueled by seasonal phytoplankton blooms, the Columbia River estuary is a natural bioreactor for organic matter transformations. Prior metagenome analyses indicated high abundances of diverse Bacteroidetes taxa in estuarine samples containing phytoplankton. To examine the hypothesis that Bacteroidetes taxa have important roles in phytoplankton turnover, we further analyzed metagenomes from water collected along a salinity gradient at 0, 5, 15, 25, and 33 PSU during bloom events. Size fractions were obtained by using a 3-μm prefilter and 0.2-μm collection filter. Although this approach targeted bacteria by removing comparatively large eukaryotic cells, the metagenome from the ES-5 sample (5 PSU) nevertheless contained an abundance of diatom DNA. Biogeochemical measurements and prior studies indicated that this finding resulted from the leakage of cellular material due to freshwater diatom lysis at low salinity. Relative to the other metagenomes, the bacterial fraction of ES-5 was dramatically depleted of genes annotated as Bacteroidetes and lysogenic bacteriophages, but was overrepresented in DNA of protists and Myxococcales bacterivores. We suggest the following equally plausible scenarios for the microbial response to phytoplankton lysis: (1) Bacteroidetes depletion in the free-living fraction may at least in part be caused by their attachment to fluvial diatoms as the latter are lysed upon contact with low-salinity estuarine waters; (2) diatom particle colonization is likely followed by rapid bacterial growth and lytic phage infection, resulting in depletion of lysogenic bacteriophages and host bacteria; and (3) the subsequent availability of labile organic matter attracted both grazers and predators to feed in this estuarine biogeochemical “hotspot,” which may have additionally depleted Bacteroidetes populations. These results represent the first detailed molecular analysis of the microbial response to phytoplankton lysis at the freshwater–brackish water interface in the fast-flowing Columbia River estuary.en_US
dc.description.sponsorshipNational Science Foundation Grant Numbers: OCE 0424602, MCB 0644468en_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sonsen_US
dc.relation.urihttps://doi.org/10.1002/mbo3.467
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBacteroidetesen_US
dc.subjectMetagenome analysisen_US
dc.subjectPhytoplankton bloom degradationen_US
dc.titleMicrobial players and processes involved in phytoplankton bloom utilization in the water column of a fast-flowing, river-dominated estuaryen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/mbo3.467


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International