Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer
Salanga, Matthew C.
Horb, Marko E.
MetadataShow full item record
Completion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated that TALENs are able to induce mutations in F0 Xenopus embryos, but none has demonstrated germline transmission of such mutations in X. laevis. In this report we used the oocyte host-transfer method to generate mutations in both tyrosinase homeologs and found highly-penetrant germline mutations; in contrast, embryonic injections yielded few germline mutations. We also compared the distribution of mutations in several F0 somatic tissues and germ cells and found that the majority of mutations in each tissue were different. These results establish that X. laevis J strain animals are very useful for generating germline mutations and that the oocyte host-transfer method is an efficient technique for generating mutations in both homeologs.
© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 188–193, doi:10.1016/j.ydbio.2016.03.006.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Inbreeding ratio and genetic relationships among strains of the Western clawed frog, Xenopus tropicalis Igawa, Takeshi; Watanabe, Ai; Suzuki, Atsushi; Kashiwagi, Akihiko; Kashiwagi, Keiko; Noble, Anna; Guille, Matthew; Simpson, David E.; Horb, Marko E.; Fujii, Tamotsu; Sumida, Masayuki (Public Library of Science, 2015-07-29)The Western clawed frog, Xenopus tropicalis, is a highly promising model amphibian, especially in developmental and physiological research, and as a tool for understanding disease. It was originally found in the West African ...
Draft genome sequence of marine alphaproteobacterial strain HIMB11, the first cultivated representative of a unique lineage within the Roseobacter clade possessing an unusually small genome Durham, Bryndan P.; Grote, Jana; Whittaker, Kerry A.; Bender, Sara J.; Luo, Haiwei; Grim, Sharon L.; Brown, Julia; Casey, John F.; Dron, Antony; Florez-Leiva, Lennis; Krupke, Andreas; Luria, Catherine M.; Mine, Aric; Nigro, Olivia D.; Pather, Santhiska; Talarmin, Agathe; Wear, Emma; Weber, Thomas S.; Wilson, Jesse; Church, Matthew J.; DeLong, Edward F.; Karl, David M.; Steward, Grieg F.; Eppley, John; Kyrpides, Nikos C.; Schuster, Stephan; Rappe, Michael S. (Genomic Standards Consortium, 2014)Strain HIMB11 is a planktonic marine bacterium isolated from coastal seawater in Kaneohe Bay, Oahu, Hawaii belonging to the ubiquitous and versatile Roseobacter clade of the alphaproteobacterial family Rhodobacteraceae. ...
Ong, Wai Kit; Vu, Trang T.; Lovendahl, Klaus N.; Llull, Jenna M.; Serres, Margrethe H.; Romine, Margaret F.; Reed, Jennifer L. (BioMed Central, 2014-03-12)Shewanella is a genus of facultatively anaerobic, Gram-negative bacteria that have highly adaptable metabolism which allows them to thrive in diverse environments. This quality makes them an attractive bacterial target for ...