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dc.contributor.authorGribble, Kristin E.  Concept link
dc.contributor.authorMark Welch, David B.  Concept link
dc.date.accessioned2017-03-20T17:10:50Z
dc.date.available2017-03-20T17:10:50Z
dc.date.issued2017-03-01
dc.identifier.citationBMC Genomics 18 (2017): 217en_US
dc.identifier.urihttps://hdl.handle.net/1912/8808
dc.description© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in BMC Genomics 18 (2017): 217, doi:10.1186/s12864-017-3540-x.en_US
dc.description.abstractUnderstanding gene expression changes over lifespan in diverse animal species will lead to insights to conserved processes in the biology of aging and allow development of interventions to improve health. Rotifers are small aquatic invertebrates that have been used in aging studies for nearly 100 years and are now re-emerging as a modern model system. To provide a baseline to evaluate genetic responses to interventions that change health throughout lifespan and a framework for new hypotheses about the molecular genetic mechanisms of aging, we examined the transcriptome of an asexual female lineage of the rotifer Brachionus manjavacas at five life stages: eggs, neonates, and early-, late-, and post-reproductive adults. There are widespread shifts in gene expression over the lifespan of B. manjavacas; the largest change occurs between neonates and early reproductive adults and is characterized by down-regulation of developmental genes and up-regulation of genes involved in reproduction. The expression profile of post-reproductive adults was distinct from that of other life stages. While few genes were significantly differentially expressed in the late- to post-reproductive transition, gene set enrichment analysis revealed multiple down-regulated pathways in metabolism, maintenance and repair, and proteostasis, united by genes involved in mitochondrial function and oxidative phosphorylation. This study provides the first examination of changes in gene expression over lifespan in rotifers. We detected differential expression of many genes with human orthologs that are absent in Drosophila and C. elegans, highlighting the potential of the rotifer model in aging studies. Our findings suggest that small but coordinated changes in expression of many genes in pathways that integrate diverse functions drive the aging process. The observation of simultaneous declines in expression of genes in multiple pathways may have consequences for health and longevity not detected by single- or multi-gene knockdown in otherwise healthy animals. Investigation of subtle but genome-wide change in these pathways during aging is an important area for future study.en_US
dc.description.sponsorshipFunding for this project was provided by R01 AG037960-01, the American Federation for Aging Research, and the Bay and Paul Foundations.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.urihttps://doi.org/10.1186/s12864-017-3540-x
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAgingen_US
dc.subjectRotiferen_US
dc.subjectMonogononten_US
dc.subjectRNA-Seqen_US
dc.subjectTranscriptomeen_US
dc.titleGenome-wide transcriptomics of aging in the rotifer Brachionus manjavacas, an emerging model systemen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s12864-017-3540-x


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International