Phase separation of signaling molecules promotes T cell receptor signal transduction
King, David S.
Rosen, Michael K.
Vale, Ronald D.
MetadataShow full item record
Activation of various cell surface receptors triggers the reorganization of downstream signaling molecules into micron- or submicron-sized clusters. However, the functional consequences of such clustering has been unclear. We biochemically reconstituted a 12-component signaling pathway on model membranes, beginning with T cell receptor (TCR) activation and ending with actin assembly. When TCR phoshophorylation was triggered, downstream signaling proteins spontaneously separated into liquid-like clusters that promoted signaling outputs both in vitro and in human Jurkat T cells. Reconstituted clusters were enriched in kinases but excluded phosphatases, and enhanced actin filament assembly by recruiting and organizing actin regulators. These results demonstrate that protein phase separation can create a distinct physical and biochemical compartment that facilitates signaling.
Author Posting. © The Author(s), 2016. This is the author's version of the work. It is posted here by permission of American Association for the Advancement of Science for personal use, not for redistribution. The definitive version was published in Science 352 (2016): 595-599, doi:10.1126/science.aad9964.
Showing items related by title, author, creator and subject.
Zimmer, Walter M. X.; Tyack, Peter L.; Johnson, Mark P.; Madsen, Peter T. (Acoustical Society of America, 2005-03)The three-dimensional beam pattern of a sperm whale (Physeter macrocephalus) tagged in the Ligurian Sea was derived using data on regular clicks from the tag and from hydrophones towed behind a ship circling the tagged ...
Performance analysis of adaptive equalization for coherent acoustic communications in the time-varying ocean environment Preisig, James C. (Acoustical Society of America, 2005-07)Equations are derived for analyzing the performance of channel estimate based equalizers. The performance is characterized in terms of the mean squared soft decision error of each equalizer. This error is decomposed into ...
Morozov, Andrey K.; Preisig, James C.; Papp, Joseph C. (Acoustical Society of America, 2008-09)Acoustical array data from the Shallow Water Acoustics experiment was processed to show the feasibility of broadband mode decomposition as a preprocessing method to reduce the effective channel delay spread and concentrate ...