Vibrio elicits targeted transcriptional responses from copepod hosts
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Copepods are abundant crustaceans that harbor diverse bacterial communities, yet the nature of their interactions with microbiota are poorly understood . Here, we report that Vibrio elicits targeted transcriptional responses in the estuarine copepod Eurytemora affinis. We pre-treated E. affinis with an antibiotic-cocktail and exposed them to either a zooplankton specialist (Vibrio sp. F10 9ZB36) or a free-living species (V. ordalii 12B09) for 24 hours. We then identified via RNA-Seq a total of 78 genes that were differentially expressed following Vibrio exposure, including homologs of C-type lectins, chitin-binding proteins and saposins. The response differed between the two Vibrio treatments, with the greatest changes elicited upon inoculation with V. sp. F10. We suggest that these differentially regulated genes play important roles in cuticle integrity, the innate immune response, and general stress responses, and that their expression may enable E. affinis to recognize and regulate symbiotic vibrios. We further report that V. sp. F10 culturability is specifically altered upon colonization of E. affinis. These findings suggest that rather than acting as passive environmental vectors, copepods discriminately interact with vibrios, which may ultimately impact the abundance and activity of copepod-associated bacteria.
Author Posting. © The Author(s), 2016. This is the author's version of the work. It is posted here by permission of Federation of European Microbiological Societies for personal use, not for redistribution. The definitive version was published in FEMS Microbiology Ecology 92 (2016): fiw072, doi:10.1093/femsec/fiw072.
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