Intracellular isotope localization in Ammonia sp. (Foraminifera) of oxygen-depleted environments : results of nitrate and sulfate labeling experiments
Bernhard, Joan M.
MetadataShow full item record
KeywordForaminifer; Nitrate; NanoSIMS; Electron dense body; Endobionts; Ultrastructure; Denitrification
Some benthic foraminiferal species are reportedly capable of nitrate storage and denitrification, however, little is known about nitrate incorporation and subsequent utilization of nitrate within their cell. In this study, we investigated where and how much 15N or 34S were assimilated into foraminiferal cells or possible endobionts after incubation with isotopically labeled nitrate and sulfate in dysoxic or anoxic conditions. After 2 weeks of incubation, foraminiferal specimens were fixed and prepared for Transmission Electron Microscopy (TEM) and correlative nanometer-scale secondary ion mass spectrometry (NanoSIMS) analyses. TEM observations revealed that there were characteristic ultrastructural features typically near the cell periphery in the youngest two or three chambers of the foraminifera exposed to anoxic conditions. These structures, which are electron dense and ~200–500 nm in diameter and co-occurred with possible endobionts, were labeled with 15N originated from 15N-labeled nitrate under anoxia and were labeled with both 15N and 34S under dysoxia. The labeling with 15N was more apparent in specimens from the dysoxic incubation, suggesting higher foraminiferal activity or increased availability of the label during exposure to oxygen depletion than to anoxia. Our results suggest that the electron dense bodies in Ammonia sp. play a significant role in nitrate incorporation and/or subsequent nitrogen assimilation during exposure to dysoxic to anoxic conditions.
© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 7 (2016): 163, doi:10.3389/fmicb.2016.00163.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Russo, Tess A.; Tully, Katherine L.; Palm, Cheryl; Neill, Christopher (Springer, 2017-05-16)Meeting food security requirements in sub-Saharan Africa (SSA) will require increasing fertilizer use to improve crop yields, however excess fertilization can cause environmental and public health problems in surface and ...
Upward nitrate transport by phytoplankton in oceanic waters : balancing nutrient budgets in oligotrophic seas Villareal, Tracy A.; Pilskaln, Cynthia H.; Montoya, Joseph P.; Dennett, Mark R. (PeerJ, 2014-03-13)In oceanic subtropical gyres, primary producers are numerically dominated by small (1–5 µm diameter) pro- and eukaryotic cells that primarily utilize recycled nutrients produced by rapid grazing turnover in a highly efficient ...
Sachs, Julian P. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1997-02)The goals of this thesis were: (1) to establish methods for the determination of nitrogen and carbon isotope ratios in marine particulate and sedimentary chlorophyll derivatives; (2) to establish chlorophyll δ15N and ...