Defining the ecological and physiological traits of phytoplankton across marine ecosystems
MetadataShow full item record
KeywordKilo Moana (Ship) Cruise KM12-19; Knorr (Ship : 1970-) Cruise KN12-17; Phytoplankton; Ecology
Marine phytoplankton are central players in the global carbon cycle, responsible for nearly half of global primary production. The identification of the factors controlling phytoplankton ecology, physiology, and, ultimately, bloom dynamics has been a central problem in the field of biological oceanography for the past century. Molecular approaches enable the direct examination of species-specific metabolic profiles in mixed, natural communities, a task which was previously intractable. In this thesis, I developed and applied novel analytical tools and bioinformatic pipelines to characterize the physiological response of phytoplankton to their environment at various levels of taxonomic grouping. An in silico Bayesian statistical approach was designed to identify stable reference genes from high-throughput sequence data for use in RT-qPCR assays or metatranscriptome studies. Using a metatranscriptomic approach, the role of resource partitioning in the coexistence of two closely related diatom species in an estuarine system was examined. This study demonstrated that co-occurring diatoms in a dynamic coastal system have apparent differences in their capacity to use nitrogen and phosphorus, and that these differences may facilitate the diversity of the phytoplankton. The second field study focused on the diatom, haptophyte, and dinoflagellate functional groups, using simulated blooms to characterize the traits that govern the magnitude and timing of phytoplankton blooms in the oligotrophic ocean. The results indicated that blooms form when phytoplankton are released from limitation by resources and that the mechanistic basis for the success of one functional group over another may be driven by how efficiently the transcriptome is modulated following a nutrient pulse. The final study looked at the sub-species level, examining the balance of phenotypic plasticity and strain diversity in the success of the coccolithophore Emiliania huxleyi. Results indicated strong control of nitrogen on the species complex and showed that nutrient resupply shifted the strain composition as well as transcript abundance of key biogeochemical genes involved in nutrient acquisition and the life stage of the population. Together, these studies demonstrate the breadth of information that can be garnered through the integration of molecular approaches with traditional biological oceanographic surveys, with each illuminating fundamental questions around phytoplankton ecology and bloom formation.
Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2016
Showing items related by title, author, creator and subject.
Clayton, Sophie A. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2013-06)The physical environment in the oceans dictates not only how phytoplankton cells are dispersed and their populations intermingled, but also mediates the supply of nutrients to the surface mixed layer. In this thesis I ...
The distribution, abundance and ecology of mixotrophic algae in marine and freshwater plankton communities Arenovski, Andrea L. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1994-09)Mixotrophic algae are algae that combine photosynthesis with phagotrophy to satisfy nutritional requirements. Mixotrophic algae have been found to dominate the nanoplankton assemblage in some aquatic environments, and ...
The ecology of colonial radiolarians : their colony morphology, trophic interactions and associations, behavior, distribution, and the photosynthesis of their symbionts Swanberg, Neil Ralph (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1979-08)Colonial radiolarians (Spumellaria) are among the most common and abundant large zooplankton, but they have been little studied by modern biologists. Colonies were found on 98% of epipelagic diving stations in the period ...