A simple novel device for air sampling by electrokinetic capture
Hampton-Marcell, Jarrad T.
Gilbert, Jack A.
MetadataShow full item record
KeywordAtomic force microscopy; Reverse transcriptase PCR; Air sampling; Field study; Aerosol; Nanoparticles; Aerobiome; Amplicon sequencing; Bacteria; Molds
A variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment. Here, we evaluate the use of a novel device, which has no technical complexity and is easily deployable. An air-cleaning device powered by electrokinetic propulsion has been adapted to provide a universal method for collecting samples of the aerobiome. Plasma-induced charge in aerosol particles causes propulsion to and capture on a counter-electrode. The flow of ions creates net bulk airflow, with no moving parts. A device and electrode assembly have been re-designed from air-cleaning technology to provide an average air flow of 120 lpm. This compares favorably with current air sampling devices based on physical air pumping. Capture efficiency was determined by comparison with a 0.4 μm polycarbonate reference filter, using fluorescent latex particles in a controlled environment chamber. Performance was compared with the same reference filter method in field studies in three different environments. For 23 common fungal species by quantitative polymerase chain reaction (qPCR), there was 100 % sensitivity and apparent specificity of 87 %, with the reference filter taken as “gold standard.” Further, bacterial analysis of 16S RNA by amplicon sequencing showed equivalent community structure captured by the electrokinetic device and the reference filter. Unlike other current air sampling methods, capture of particles is determined by charge and so is not controlled by particle mass. We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air. This work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range. The performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.
© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Microbiome 3 (2015): 79, doi:10.1186/s40168-015-0141-2.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Rapid extraction of dissolved inorganic carbon from seawater and groundwater samples for radiocarbon dating Gospodinova, Kalina D.; McNichol, Ann P.; Gagnon, Alan R.; Shah Walter, Sunita R. (John Wiley & Sons, 2015-10-29)We designed and developed a system to efficiently extract dissolved inorganic carbon (DIC) from seawater and groundwater samples for radiocarbon dating. The Rapid Extraction of Dissolved Inorganic Carbon System (REDICS) ...
Brook, Edward J.; Kurz, Mark D.; Curtice, Joshua (2009-07-12)Accretion of extraterrestrial material to earth is of interest for a variety of reasons, including as a possible driver of long or short-term climate change, and as a record of solar system events preserved in the ...
Broda, James E.; Andrew, P. J. (Woods Hole Oceanographic Institution, 1986-09)This report is Volume II in the series of reports entitled "DESCRIPTIONS OF W.H.O.I. ROCK DREDGE SAMPLES". This volume represents the final step in the major effort to catalog and prepare initial descriptions for all ...