A simple novel device for air sampling by electrokinetic capture
Hampton-Marcell, Jarrad T.
Gilbert, Jack A.
MetadataShow full item record
KeywordAtomic force microscopy; Reverse transcriptase PCR; Air sampling; Field study; Aerosol; Nanoparticles; Aerobiome; Amplicon sequencing; Bacteria; Molds
A variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment. Here, we evaluate the use of a novel device, which has no technical complexity and is easily deployable. An air-cleaning device powered by electrokinetic propulsion has been adapted to provide a universal method for collecting samples of the aerobiome. Plasma-induced charge in aerosol particles causes propulsion to and capture on a counter-electrode. The flow of ions creates net bulk airflow, with no moving parts. A device and electrode assembly have been re-designed from air-cleaning technology to provide an average air flow of 120 lpm. This compares favorably with current air sampling devices based on physical air pumping. Capture efficiency was determined by comparison with a 0.4 μm polycarbonate reference filter, using fluorescent latex particles in a controlled environment chamber. Performance was compared with the same reference filter method in field studies in three different environments. For 23 common fungal species by quantitative polymerase chain reaction (qPCR), there was 100 % sensitivity and apparent specificity of 87 %, with the reference filter taken as “gold standard.” Further, bacterial analysis of 16S RNA by amplicon sequencing showed equivalent community structure captured by the electrokinetic device and the reference filter. Unlike other current air sampling methods, capture of particles is determined by charge and so is not controlled by particle mass. We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air. This work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range. The performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.
© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Microbiome 3 (2015): 79, doi:10.1186/s40168-015-0141-2.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Autonomous and remotely operated vehicle technology for hydrothermal vent discovery, exploration, and sampling Yoerger, Dana R.; Bradley, Albert M.; Jakuba, Michael V.; German, Christopher R.; Shank, Timothy M.; Tivey, Maurice A. (Oceanography Society, 2007-03)Autonomous and remotely operated underwater vehicles play complementary roles in the discovery, exploration, and detailed study of hydrothermal vents. Beginning with clues provided by towed or lowered instruments, ...
Characterization of underwater target geometry from Autonomous Underwater Vehicle sampling of bistatic acoustic scattered fields Fischell, Erin M. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2015-06)One of the long term goals of Autonomous Underwater Vehicle (AUV) minehunting is to have multiple inexpensive AUVs in a harbor autonomously classify hazards. Existing acoustic methods for target classification using ...
Data file, Continental Margin Program, Atlantic Coast of the United States : vol. 2 sample collection and analytical data Hathaway, John C. (Woods Hole Oceanographic Institution, 1971-02)The purpose of the data file presented below is twofold: the first purpose is to make available in printed form the basic data relating to the samples collected as part of the joint U.S. Geological Survey - Woods Hole ...