Destructive changes in the neuronal structure of the FVB/N mouse retina
MetadataShow full item record
We applied a series of selective antibodies for labeling the various cell types in the mammalian retina. These were used to identify the progressive loss of neurons in the FVB/N mouse, a model of early onset retinal degeneration produced by a mutation in the pde6b gene. The immunocytochemical studies, together with electroretinogram (ERG) recordings, enabled us to examine the time course of the degenerative changes that extended from the photoreceptors to the ganglion cells at the proximal end of the retina. Our study indicates that photoreceptors in FVB/N undergo a rapid degeneration within three postnatal weeks, and that there is a concomitant loss of retinal neurons in the inner nuclear layer. Although the loss of rods was detected at an earlier age during which time M- and S-opsin molecules were translocated to the cone nuclei; by 6 months all cones had also degenerated. Neuronal remodeling was also seen in the second-order neurons with horizontal cells sprouting processes proximally and dendritic retraction in rod-driven bipolar cells. Interestingly, the morphology of cone-driven bipolar cells were affected less by the disease process. The cellular structure of inner retinal neurons, i.e., ChAT amacrine cells, ganglion cells, and melanopsin-positive ganglion cells did not exhibit any gross changes of cell densities and appeared to be relatively unaffected by the massive photoreceptor degeneration in the distal retina. However, Muller cell processes began to express GFAP at their endfeet at p14, and it climbed progressively to the cell’s distal ends by 6 months. Our study indicates that FVB/N mouse provides a useful model with which to assess possible intervention strategies to arrest photoreceptor death in related diseases.
© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0129719, doi: 10.1371/journal.pone.0129719.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Global economic effects of changes in crops, pasture, and forests due to changing climate, carbon dioxide, and ozone Reilly, John M.; Paltsev, S.; Felzer, Benjamin S.; Wang, X.; Kicklighter, David W.; Melillo, Jerry M.; Prinn, Ronald G.; Sarofim, Marcus C.; Sokolov, Andrei P.; Wang, C. (2006-01)Multiple environmental changes will have consequences for global vegetation. To the extent that crop yields and pasture and forest productivity are affected there can be important economic consequences. We examine the ...
Abrupt climate change as an important agent of ecological change in the Northeast U.S. throughout the past 15,000 years Shuman, Bryan N.; Newby, Paige E.; Donnelly, Jeffrey P. (2009-03-30)We use a series of tests to evaluate two competing hypotheses about the association of climate and vegetation trends in the northeastern United States over the past 15 kyrs. First, that abrupt climate changes on the scale ...
Marine Biological Laboratory; Woods Hole Oceanographic Institution (2009)Observations show that climate warming is already having significant impacts on the ocean. How the ocean and sea ice respond will determine the trajectory of future global climate.