Genealogical analyses of multiple loci of litostomatean ciliates (Protista, Ciliophora, Litostomatea)
Bourland, William A.
Orsi, William D.
Epstein, Slava S.
MetadataShow full item record
KeywordBody polarization; ITS1-5.8S rRNA-ITS2 region sequences; Motif; Oral simplification; Secondary structure
The class Litostomatea is a highly diverse ciliate taxon comprising hundreds of free-living and endocommensal species. However, their traditional morphology-based classification conflicts with 18S rRNA gene phylogenies indicating (1) a deep bifurcation of the Litostomatea into Rhynchostomatia and Haptoria + Trichostomatia, and (2) body polarization and simplification of the oral apparatus as main evolutionary trends in the Litostomatea. To test whether 18S rRNA molecules provide a suitable proxy for litostomatean evolutionary history, we used eighteen new ITS1-5.8S rRNA-ITS2 region sequences from various free-living litostomatean orders. These single- and multiple-locus analyses are in agreement with previous 18S rRNA gene phylogenies, supporting that both 18S rRNA gene and ITS region sequences are effective tools for resolving phylogenetic relationships among the litostomateans. Despite insertions, deletions and mutational saturations in the ITS region, the present study shows that ITS1 and ITS2 molecules can be used to infer phylogenetic relationships not only at species level but also at higher taxonomic ranks when their secondary structure information is utilized to aid alignment.
© The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Phylogenetics and Evolution 66 (2012): 397-411, doi:10.1016/j.ympev.2012.06.024.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Unported
Showing items related by title, author, creator and subject.
Williams, William J. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1996-02)During January-March, Scotian Shelf water has been observed to flow episodically from the southwestern Scotian Shelf directly across the Northeast Channel to Georges Bank. The possible factors that allow Scotian Shelf ...
Fundulus as the premier teleost model in environmental biology : opportunities for new insights using genomics Burnett, Karen G.; Bain, Lisa J.; Baldwin, William S.; Callard, Gloria V.; Cohen, Sarah; Di Giulio, Richard T.; Evans, David H.; Gomez-Chiarri, Marta; Hahn, Mark E.; Hoover, Cindi A.; Karchner, Sibel I.; Katoh, Fumi; MacLatchy, Deborah L.; Marshall, William S.; Meyer, Joel N.; Nacci, Diane E.; Oleksiak, Marjorie F.; Rees, Bernard B.; Singer, Thomas D.; Stegeman, John J.; Towle, David W.; Van Veld, Peter A.; Vogelbein, Wolfgang K.; Whitehead, Andrew; Winn, Richard N.; Crawford, Douglas L. (2007-09-01)A strong foundation of basic and applied research documents that the estuarine fish Fundulus heteroclitus and related species are unique laboratory and field models for understanding how individuals and populations interact ...
From salty to fresh—salinity processes in the Upper-ocean Regional Study-2 (SPURS-2) : diagnosing the physics of a rainfall-dominated salinity minimum Schmitt, Raymond W.; Asher, William; Bingham, Frederick; Carton, James A.; Centurioni, Luca R.; Farrar, J. Thomas; Gordon, Arnold L.; Hodges, Benjamin A.; Jessup, Andrew T.; Kessler, William S.; Rainville, Luc; Shcherbina, Andrey Y. (The Oceanography Society, 2015-03)One of the notable features of the global ocean is that the salinity of the North Atlantic is about 1 psu higher than that of the North Pacific. This contrast is thought to be due to one of the large asymmetries in the ...