The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios
Iglesias-Rodriguez, M. Debora
MetadataShow full item record
KeywordEmiliania huxleyi; Ocean acidification; Nutrients; Alkaline phosphatase; Nitrate reductase; Calcification
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 μatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 μatm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification.
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 4 (2013): 155, doi:10.3389/fmicb.2013.00155.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Kendrick, B. Jacob; DiTullio, Giacomo R.; Cyronak, Tyler J.; Fulton, James M.; Van Mooy, Benjamin A. S.; Bidle, Kay D. (Public Library of Science, 2014-11-18)Annual Emiliania huxleyi blooms (along with other coccolithophorid species) play important roles in the global carbon and sulfur cycles. E. huxleyi blooms are routinely terminated by large, host-specific dsDNA viruses, ...
A bacterial quorum-sensing precursor induces mortality in the marine coccolithophore, Emiliania huxleyi Harvey, Elizabeth L.; Deering, Robert W.; Rowley, David C.; El Gamal, Abrahim; Schorn, Michelle A.; Moore, Bradley S.; Johnson, Matthew D.; Mincer, Tracy J.; Whalen, Kristen E. (Frontiers Media, 2016-02-03)Interactions between phytoplankton and bacteria play a central role in mediating biogeochemical cycling and food web structure in the ocean. However, deciphering the chemical drivers of these interspecies interactions ...
Consequences of strain variability and calcification in Emiliania huxleyi on microzooplankton grazing Harvey, Elizabeth L.; Bidle, Kay D.; Johnson, Matthew D. (2015-08-04)Microzooplankton are the main consumers of marine phytoplankton. Intrinsic traits of phytoplankton can reduce grazing mortality, directly influencing phytoplankton population dynamics, food web ecology, and biogeochemical ...