Active eukaryotes in microbialites from Highborne Cay, Bahamas, and Hamelin Pool (Shark Bay), Australia
Edgcomb, Virginia P.
Bernhard, Joan M.
Summons, Roger E.
Orsi, William D.
Beaudoin, David J.
Visscher, Pieter T.
MetadataShow full item record
KeywordEukaryote; Protist; Stromatolite; Microbialite; Thrombolite; 18S rRNA; Diversity; Foraminifera; Hamelin Pool; Highborne Cay
Microbialites are organosedimentary structures that are formed through the interaction of benthic microbial communities and sediments and include mineral precipitation. These lithifying microbial mat structures include stromatolites and thrombolites. Exuma Sound in the Bahamas, and Hamelin Pool in Shark Bay, Western Australia are two locations where significant stands of modern microbialites exist. Although prokaryotic diversity in these structures is reasonably well documented, little is known about the eukaryotic component of these communities and their potential to influence sedimentary fabrics through grazing, binding and burrowing activities. Accordingly, comparisons of eukaryotic communities in modern stromatolitic and thrombolytic mats can potentially provide insight into the coexistence of both laminated and clotted mat structures in close proximity to one another. Here we examine this possibility by comparing eukaryotic diversity based on Sanger and high-throughput pyrosequencing of small subunit ribosomal RNA (18S rRNA) genes. Analyses were based on total RNA extracts as template to minimize input from inactive or deceased organisms. Results identified diverse eukaryotic communities particularly stramenopiles, Alveolata, Metazoa, Amoebozoa, and Rhizaria within different mat types at both locations, as well as abundant and diverse signatures of eukaryotes with <80% sequence similarity to sequences in GenBank. This suggests presence of significant novel eukaryotic diversity, particularly in hypersaline Hamelin Pool. There was evidence of vertical structuring of protist populations and foraminiferal diversity was highest in bioturbated/clotted thrombolite mats of Highborne Cay.
Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in The ISME Journal 8 (2014): 418–429, doi:10.1038/ismej.2013.130.
Showing items related by title, author, creator and subject.
The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP) : illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing Keeling, Patrick J.; Burki, Fabien; Wilcox, Heather M.; Allam, Bassem; Allen, Eric E.; Amaral-Zettler, Linda A.; Armbrust, E. Virginia; Archibald, John M.; Bharti, Arvind K.; Bell, Callum J.; Beszteri, Bank; Bidle, Kay D.; Cameron, Connor T.; Campbell, Lisa; Caron, David A.; Cattolico, Rose Ann; Collier, Jackie L.; Coyne, Kathryn J.; Davy, Simon K.; Deschamps, Phillipe; Dyhrman, Sonya T.; Edvardsen, Bente; Gates, Ruth D.; Gobler, Christopher J.; Greenwood, Spencer J.; Guida, Stephanie M.; Jacobi, Jennifer L.; Jakobsen, Kjetill S.; James, Erick R.; Jenkins, Bethany D.; John, Uwe; Johnson, Matthew D.; Juhl, Andrew R.; Kamp, Anja; Katz, Laura A.; Kiene, Ronald P.; Kudryavtsev, Alexander N.; Leander, Brian S.; Lin, Senjie; Lovejoy, Connie; Lynn, Denis; Marchetti, Adrian; McManus, George; Nedelcu, Aurora M.; Menden-Deuer, Susanne; Miceli, Cristina; Mock, Thomas; Montresor, Marina; Moran, Mary Ann; Murray, Shauna A.; Nadathur, Govind; Nagai, Satoshi; Ngam, Peter B.; Palenik, Brian; Pawlowski, Jan; Petroni, Giulio; Piganeau, Gwenael; Posewitz, Matthew C.; Rengefors, Karin; Romano, Giovanna; Rumpho, Mary E.; Rynearson, Tatiana A.; Schilling, Kelly B.; Schroeder, Declan C.; Simpson, Alastair G. B.; Slamovits, Claudio H.; Smith, David R.; Smith, G. Jason; Smith, Sarah R.; Sosik, Heidi M.; Stief, Peter; Theriot, Edward; Twary, Scott N.; Umale, Pooja E.; Vaulot, Daniel; Wawrik, Boris; Wheeler, Glen L.; Wilson, William H.; Xu, Yan; Zingone, Adriana; Worden, Alexandra Z. (Public Library of Science, 2014-06-24)Microbial ecology is plagued by problems of an abstract nature. Cell sizes are so small and population sizes so large that both are virtually incomprehensible. Niches are so far from our everyday experience as to make their ...
Arkhipova, Irina R. (Oxford University Press, 2006-10)Penelope-like elements (PLEs) are a relatively little studied class of eukaryotic retroelements, distinguished by the presence of the GIY-YIG endonuclease domain, the ability of some representatives to retain introns, and ...
Distribution and seasonal variability in the benthic eukaryotic community of Río Tinto (SW, Spain), an acidic, high metal extreme environment Aguilera, Angeles; Zettler, Erik R.; Gomez, Felipe; Amaral-Zettler, Linda A.; Rodríguez, Nuria; Amils, Ricardo (2007-02-14)The eukaryotic community of Río Tinto (SW, Spain) was surveyed fall, winter, and spring through the combined use of traditional microscopy and molecular approaches including Denaturing Gradient Gel Electrophoresis (DGGE) ...