Introducing Toxicology into the Biochemistry Curricula: Using Cytochrome c (Cytc) Functionalities as a Model
MetadataShow full item record
The electron transport chain (ETC) is a keystone topic of all biochemistry courses at the undergraduate level. Many ETC components, especially cytochrome c (Cytc), are also important to the field of toxicology. Unfortunately, many primarily undergraduate institutions (PUIs) are unable to offer dedicated toxicology courses and laboratories due to faculty expertise and/or enrollment requirements. In an effort to provide chemistry and biology undergraduates with toxicology perspective and experience, I have integrated Cytc toxicology, and its role in apoptosis, into my Biochemistry I and II curriculum. This approach fulfills two goals: 1) integration of toxicology concepts into the biochemistry curriculum and 2) validation of fundamental biochemistry principles through demonstration of “real world” relevance in the field of toxicology. These concepts include Cytc “leakage” to the cytosol, activation of the apoptotic signaling cascade, Cytc/membrane interactions, the modulation of apoptosis by Cytc phosphorylation, and chemical/environmental toxicants that activate this function of Cytc. I conclude with a discussion of student assessment in relation to this methodology. Overall, these materials provide biochemistry instructors with a primer to introduce toxicology concepts in the greater biochemistry curricula or a means for toxicology faculty to validate key biochemistry principles within their classroom.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States
Showing items related by title, author, creator and subject.
Diversity and characterization of novel Cytochrome P450 2 genes in the marine teleost Fundulus Heteroclitus Oleksiak, Marjorie F. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1997-09)The multiplicity and roles of cytochrome P450 CYP genes in the 2 family are not well known in fish. Characterizations of CYP2 genes were done in the killifish, Fundulus heteroclitus. Multiple cDNAs from three CYP2 ...
Involvement of Cytochrome P450 1A in the toxicity of aryl hydrocarbon receptor agonists : alteration arachidonic acid metabolism and production of reactive oxygen species Schlezinger, Jennifer J. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1998-08)Two cytochrome P4501A-dependent mechanisms of aryl hydrocarbon receptor (AhR) agonist toxicity were examined in the marine teleost scup (Stenotomus chrysops), alteration of arachidonic acid (AA) metabolism and production ...
Sussman, Raquel (American Society for Biochemistry and Molecular Biology, 2008-12-15)As I look back, I realize that serendipity has played a major role in my life. I grew up in Santiago, Chile. As far back as I can remember I was interested in plants and animals, enjoying my Aunt Olga's farm, where I could ...