Developmental expression of the Nfe2-related factor (Nrf) transcription factor family in the zebrafish, Danio rerio
Williams, Larissa M.
Timme-Laragy, Alicia R.
Goldstone, Jared V.
McArthur, Andrew G.
Stegeman, John J.
Smolowitz, Roxanna M.
Hahn, Mark E.
MetadataShow full item record
Transcription factors in the CNC-bZIP family (NFE2, NRF1, NRF2 and NRF3) regulate genes with a wide range of functions in response to both physiological and exogenous signals, including those indicating changes in cellular redox status. Given their role in helping to maintain cellular homeostasis, it is imperative to understand the expression, regulation, and function of CNC-bZIP genes during embryonic development. We explored the expression and function of six nrf genes (nfe2, nrf1a, nrf1b, nrf2a, nrf2b, and nrf3) using zebrafish embryos as a model system. Analysis by microarray and quantitative RT-PCR showed that genes in the nrf family were expressed throughout development from oocytes to larvae. The spatial expression of nrf3 suggested a role in regulating the development of the brain, brachia and pectoral fins. Knock-down by morpholino anti-sense oligonucleotides suggested that none of the genes were necessary for embryonic viability, but nfe2 was required for proper cellular organization in the pneumatic duct and subsequent swim bladder function, as well as for proper formation of the otic vesicles. nrf genes were induced by the oxidant tert-butylhydroperoxide, and some of this response was regulated through family members Nrf2a and Nrf2b. Our results provide a foundation for understanding the role of nrf genes in normal development and in regulating the response to oxidative stress in vertebrate embryos.
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 8 (2013): e79574, doi:10.1371/journal.pone.0079574.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Serum resistance-associated protein blocks lysosomal targeting of trypanosome lytic factor in Trypanosoma brucei Oli, Monika W.; Cotlin, Laura F.; Shiflett, April M.; Hajduk, Stephen L. (American Society for Microbiology, 2006-01)Trypanosoma brucei brucei is the causative agent of nagana in cattle and can infect a wide range of mammals but is unable to infect humans because it is susceptible to the innate cytotoxic activity of normal human serum. ...
Burbach, J. Peter H.; Hellemons, Anita J. C. G. M.; Grant, Philip; Pant, Harish C. (The Company of Biologists, 2015-06-26)Homeodomain transcription factors regulate development of embryos and cellular physiology in adult systems. Paired-type homeodomain genes constitute a subclass that has been particularly implicated in establishment of ...
Groen, Aaron C.; Maresca, Thomas J.; Gatlin, Jesse C.; Salmon, Edward D.; Mitchison, Timothy J. (American Society for Cell Biology, 2009-04-15)Distinct pathways from centrosomes and chromatin are thought to contribute in parallel to microtubule nucleation and stabilization during animal cell mitotic spindle assembly, but their full mechanisms are not known. We ...